: Can is the high threshold, long-lasting calcium current in Schild 1994
NEURON {
SUFFIX acan
USEION ca READ cao, cai WRITE ica
RANGE gbar, ecan, ica
}
UNITS {
(S) = (siemens)
(mV) = (millivolts)
(mA) = (milliamp)
F = (faraday) (coulombs)
(molar) = (1/liter)
(mM) = (millimolar)
}
PARAMETER {
gbar =0.000795775 (S/cm2): (S/cm2)
Q10can=4.30 :Each gating variable has the same constant
V0p5d=-20.0 (mV):As defined by Schild 1994, zinf=1.0/(1.0+exp((V0p5z-V)/S0p5z)
S0p5d=4.5 (mV)
V0p5f1=-20.0 (mV)
S0p5f1=-25.0 (mV)
V0p5f2=-40.0 (mV)
S0p5f2=-10.0 (mV)
A_taud=3.25 (ms) :As defined by Schild 1994, tauz=A_tauz*exp(-B^2(V-Vpz)^2)+C
B_taud=0.042 (/mV)
C_taud=0.395 (ms)
Vpd=-31.0 (mV)
A_tauf1=33.5 (ms)
B_tauf1=.0395 (/mV)
C_tauf1=5.0 (ms)
Vpf1=-30.0 (mV)
A_tauf2=225.0 (ms)
B_tauf2=0.0275 (/mV)
C_tauf2=75.00 (ms)
Vpf2=-40.0 (mV)
A_rn=5.0 (mV)
B_rn=-10.0 (mV)
R=8.314 (joule/degC): Gas Constant
z=2 : Charge of Ca ion
ecaoffset=78.7 (mV)
}
COMMENT
The above Q10 constants were given in Schild 1994 with no indication of how they
were implemented. It was decided, based on the value of the Q10 constants given,
that the most likely answer was that the tau of each gating variable was divided by
the Q10. This is reflected below where tau_x=tau_x*(1/Q10x). Note that Schild only
provides a single constant, not any type of equation for q10. The equations are orginally
given for 22C, and this constant changes the equation to 37C.
ENDCOMMENT
ASSIGNED {
v (mV) : NEURON provides this
ica (mA/cm2)
celsius (degC)
g (S/cm2)
tau_f1 (ms)
tau_d (ms)
tau_f2 (ms)
dinf
f1inf
f2inf
rn
ecan (mV)
cao (mM)
cai (mM)
}
STATE { d f1 f2 }
BREAKPOINT {
SOLVE states METHOD cnexp
g = gbar * d * (0.55*f1+0.45*f2)
ica = g * (v-ecan)
}
INITIAL {
rates(v) : set tau_m, tau_h, hinf, minf
: assume that equilibrium has been reached
d = dinf
f1 = f1inf
f2 = f2inf
}
DERIVATIVE states {
rates(v)
d' = (dinf - d)/tau_d
f1' = (f1inf - f1)/tau_f1
f2' = (f2inf - f2)/tau_f2
}
FUNCTION rates(Vm (mV)) (/ms) {
rn=0.2/(1.0+exp((Vm +A_rn)/B_rn))
tau_d = A_taud*exp(-(B_taud)^2*(Vm-Vpd)^2)+C_taud
dinf = 1.0/(1.0+exp((Vm-V0p5d)/(-S0p5d)))
tau_f1 = A_tauf1*exp(-(B_tauf1)^2*(Vm-Vpf1)^2)+C_tauf1
f1inf = 1.0/(1.0+exp((Vm-V0p5f1)/(-S0p5f1)))
tau_f2 = A_tauf2*exp(-(B_tauf2)^2*(Vm-Vpf2)^2)+C_tauf2
f2inf =rn+(1.0/(1.0+exp((Vm-V0p5f2)/(-S0p5f2))))
ecan=(1000)*(R*(celsius+273.15)/z/F*log(cao/cai))-ecaoffset : Equation for eca given in Schild 1994
if (celsius >= 37) {
tau_d=tau_d*(1/Q10can)
tau_f1=tau_f1*(1/Q10can)
tau_f2=tau_f2*(1/Q10can)
}
}