# -*- coding: utf-8 -*-
from neuron import h
import numpy as np
import random
import math
import sys
from random import choice
from Purkinje_morpho_1_number import number_ind_1
from Synapses import Synapse_py3
class Purkinje_Morpho_1:
def __init__(self,spines_on = 0):
h.load_file('stdlib.hoc')
h.load_file('import3d.hoc')
cell = h.Import3d_Neurolucida3()
cell.input('morphology/Human_PC.asc')
i3d = h.Import3d_GUI(cell,0)
i3d.instantiate(self)
conductvalues = np.genfromtxt("R_01_final_pop.txt")
indiv_number = number_ind_1['indiv']
print( 'indiv number', indiv_number)
self.soma[0].nseg = 1 + (2 * int(self.soma[0].L / 40))
self.soma[0].cm = 1
self.soma[0].Ra = 122
self.soma[0].insert('Leak')
self.soma[0].e_Leak = -61
self.soma[0].gmax_Leak = 1E-3
self.soma[0].insert('Nav1_6')
self.soma[0].gbar_Nav1_6 = conductvalues[indiv_number, 14]
self.soma[0].ena = 60
self.soma[0].insert('Kv1_1')
self.soma[0].gbar_Kv1_1 = conductvalues[indiv_number, 15]
self.soma[0].insert('Kv1_5')
self.soma[0].gKur_Kv1_5 = conductvalues[indiv_number, 35]
self.soma[0].insert('Kv3_4')
self.soma[0].gkbar_Kv3_4 = conductvalues[indiv_number, 16]
self.soma[0].insert('Kir2_3')
self.soma[0].gkbar_Kir2_3 = conductvalues[indiv_number, 17]
self.soma[0].insert('Cav2_1')
self.soma[0].pcabar_Cav2_1 = conductvalues[indiv_number,18]
self.soma[0].insert('Cav3_1')
self.soma[0].pcabar_Cav3_1 = conductvalues[indiv_number,19]
self.soma[0].insert('Cav3_2')
self.soma[0].gcabar_Cav3_2 =conductvalues[indiv_number,20]
self.soma[0].insert('Cav3_3')
self.soma[0].pcabar_Cav3_3 = conductvalues[indiv_number,21]
self.soma[0].insert('Kca1_1')
self.soma[0].gbar_Kca1_1 = conductvalues[indiv_number,22]
self.soma[0].insert('Kca2_2')
self.soma[0].gkbar_Kca2_2 = conductvalues[indiv_number,23]
self.soma[0].insert('Kca3_1')
self.soma[0].gkbar_Kca3_1 = conductvalues[indiv_number,24]
self.soma[0].ek = -88
self.soma[0].insert('HCN1')
self.soma[0].gbar_HCN1 = conductvalues[indiv_number,25]*0.5
self.soma[0].eh = -34.4
self.soma[0].insert('cdp5_CAM')
self.soma[0].TotalPump_cdp5_CAM = 5e-8
self.soma[0].push()
self.soma[0].eca = 137.52625
h.pop_section()
self.whatami = "prk"
#Dend coordinate
for d in self.dend:
d.Ra = 122
if spines_on == 1:
d.cm = 2
if spines_on == 0:
d.cm = (11.510294 * math.exp( - 1.376463 * d.diam) + 2.120503)
d.nseg = 1 + (2 * int(d.L / 40))
d.insert('Leak')
d.e_Leak = -61
d.gmax_Leak = 0.0003
d.insert('Kv3_3')
d.gbar_Kv3_3 = conductvalues[indiv_number,3]
d.insert('Kv4_3')
d.gkbar_Kv4_3 = conductvalues[indiv_number,4]
d.insert('Cav2_1')
d.pcabar_Cav2_1 = conductvalues[indiv_number,6]
d.insert('Cav3_3')
d.pcabar_Cav3_3 = conductvalues[indiv_number,9]
d.insert('Kca1_1')
d.gbar_Kca1_1 = conductvalues[indiv_number,10]
d.insert('HCN1')
d.gbar_HCN1 = conductvalues[indiv_number,13]
d.eh = -34.4
d.insert('Kca2_2')
d.gkbar_Kca2_2 = conductvalues[indiv_number,11]
d.insert('cdp5_CAM')
d.TotalPump_cdp5_CAM = 5e-8
if d.diam >= 1.6:
d.cm = 2
d.insert('Kv1_1')
d.gbar_Kv1_1 = conductvalues[indiv_number,1]
d.insert('Kv1_5')
d.gKur_Kv1_5 = conductvalues[indiv_number,2]
d.insert('Kir2_3')
d.gkbar_Kir2_3 = conductvalues[indiv_number,5]
d.insert('Cav3_1')
d.pcabar_Cav3_1 = conductvalues[indiv_number,7]
d.insert('Cav3_2')
d.gcabar_Cav3_2 = conductvalues[indiv_number,8]
d.insert('Kca3_1')
d.gkbar_Kca3_1 = conductvalues[indiv_number,12]
if d.diam >=3.3:
d.cm = 2
d.insert('Nav1_6')
d.gbar_Nav1_6 = conductvalues[indiv_number,0]
d.ena = 60
d.ek = -88
d.push()
d.eca = 137.52625
h.pop_section()
#Axon AIS. First section after the soma[0]
self.axonAIS = h.Section(name='axonAIS')
self.axonAIS.nseg = 1
self.axonAIS.diam = 0.97
self.axonAIS.cm = 1
self.axonAIS.L = 17
self.axonAIS.Ra = 122
self.axonAIS.insert('Leak')
self.axonAIS.e_Leak = -61
self.axonAIS.gmax_Leak = 0.0003
self.axonAIS.insert('Nav1_6')
self.axonAIS.gbar_Nav1_6 = 1.8 #conductvalues[indiv_number,26] mmodified to have a lower responce
self.axonAIS.ena = 60
self.axonAIS.insert('Kv3_4')
self.axonAIS.gkbar_Kv3_4 = conductvalues[indiv_number,27]
self.axonAIS.ek = -88
self.axonAIS.insert('Cav2_1')
self.axonAIS.pcabar_Cav2_1 = conductvalues[indiv_number,28]
self.axonAIS.insert('Cav3_1')
self.axonAIS.pcabar_Cav3_1 = conductvalues[indiv_number,29]
self.axonAIS.insert('cdp5_CAM')
self.axonAIS.TotalPump_cdp5_CAM = 2e-8
self.axonAIS.push()
self.axonAIS.eca = 137.52625
h.pop_section()
#AISK
self.axonAISK = h.Section(name='axonAISK')
self.axonAISK.nseg = 1
self.axonAISK.diam = 0.97
self.axonAISK.cm = 1
self.axonAISK.L = 4
self.axonAISK.Ra = 122
self.axonAISK.insert('Leak')
self.axonAISK.e_Leak = -61
self.axonAISK.gmax_Leak = 0.0003
self.axonAISK.insert('Kv1_1')
self.axonAISK.gbar_Kv1_1 = conductvalues[indiv_number,30]
self.axonAISK.ek = -88
#First Myelination
self.axonmyelin = h.Section(name='axonmyelin')
self.axonmyelin.nseg = 1
self.axonmyelin.diam = 0.73
self.axonmyelin.L = 100 #da 60 a 150um
self.axonmyelin.insert('pas') #from brill77
self.axonmyelin.e_pas = -61 #from brill77
self.axonmyelin.g_pas = 5.60e-9 #from brill77
self.axonmyelin.cm = 1.87e-11 #from brill77
self.axonmyelin.Ra = 122
#NEW NODES of RANVIER
secnumber = 3
#The IN paranode
self.renv_node = [h.Section(name='renv_node'+str(x)) for x in range(secnumber)]
for a in self.renv_node:
a.nseg = 1
a.diam = 0.73
a.cm = 1
a.L = 4
a.Ra = 122
a.insert('Leak')
a.e_Leak = -61
a.gmax_Leak = 0.0003
a.insert('Nav1_6')
a.gbar_Nav1_6 = conductvalues[indiv_number,31]
a.ena = 60
a.insert('Kv3_4')
a.gkbar_Kv3_4 = conductvalues[indiv_number,32]
a.ek = -88
a.insert('Cav2_1')
a.pcabar_Cav2_1 = conductvalues[indiv_number,33]
a.insert('Cav3_1')
a.pcabar_Cav3_1 = conductvalues[indiv_number,34]
a.insert('cdp5_CAM')
a.TotalPump_cdp5_CAM = 5e-7
a.push()
a.eca = 137.52625
h.pop_section()
#Myelin sections
self.axoninode = [h.Section(name='axoninode'+str(x)) for x in range(secnumber)]
for d in self.axoninode:
d.nseg = 1 #+ (2 * int(d.L / 40))
d.diam = 0.73
d.L = 100
d.insert('pas') #from brill77
d.e_pas = -61 #from brill77
d.g_pas = 5.60e-9 #from brill77
d.cm = 1.87e-11 #from brill77
d.Ra = 122
#Collateral.
self.axoncoll = h.Section(name='axoncoll')
self.axoncoll.nseg = 1
self.axoncoll.diam = 0.6
self.axoncoll.L = 100
self.axoncoll.insert('Leak')
self.axoncoll.e_Leak = -61
self.axoncoll.gmax_Leak = 0.0003
self.axoncoll.insert('Nav1_6')
self.axoncoll.gbar_Nav1_6 = 0.03
self.axoncoll.ena = 60
self.axoncoll.insert('Kv3_4')
self.axoncoll.gkbar_Kv3_4 = 0.02
self.axoncoll.ek = -88
self.axoncoll.insert('Cav3_1')
self.axoncoll.pcabar_Cav3_1 = 1e-5
self.axoncoll.insert('Cav2_1')
self.axoncoll.pcabar_Cav2_1 = 2.2e-4
self.axoncoll.insert('cdp5_CAM')
self.axoncoll.TotalPump_cdp5_CAM = 5e-7
#Collateral second part
self.axoncoll2 = h.Section(name='axoncoll2')
self.axoncoll2.nseg = 1
self.axoncoll2.diam = 0.6
self.axoncoll2.L = 100
#self.axoncoll2.Ra = 122
self.axoncoll2.insert('Leak')
self.axoncoll2.e_Leak = -61
self.axoncoll2.gmax_Leak = 0.0003
self.axoncoll2.insert('Nav1_6')
self.axoncoll2.gbar_Nav1_6 = 0.03
self.axoncoll2.ena = 60
self.axoncoll2.insert('Kv3_4')
self.axoncoll2.gkbar_Kv3_4 = 0.02
self.axoncoll2.ek = -88
self.axoncoll2.insert('cdp5_CAM')
self.axoncoll2.insert('Cav3_1')
self.axoncoll2.pcabar_Cav3_1 = 1e-5
self.axoncoll2.insert('Cav2_1')
self.axoncoll2.pcabar_Cav2_1 = 2.2e-4
self.axoncoll2.TotalPump_cdp5_CAM = 5e-7
self.axonAIS.connect(self.soma[0],0,0)
self.axonAISK.connect(self.axonAIS,1,0)
self.axonmyelin.connect(self.axonAISK,1,0) #myelin1
self.renv_node[0].connect(self.axonmyelin,1,0) #node1
self.axoninode[0].connect(self.renv_node[0],1,0) #myelin2
self.renv_node[1].connect(self.axoninode[0],1,0) #node2
self.axoninode[1].connect(self.renv_node[1],1,0) #myelin3
self.renv_node[2].connect(self.axoninode[1],1,0) #node3
self.axoninode[2].connect(self.renv_node[2],1,0) #myelin4
self.axoncoll.connect(self.renv_node[1],1,0)
self.axoncoll2.connect(self.axoncoll,1,0)
if spines_on == 1:
dend_index = []
dend_local_len = []
dend_len = []
spine_location = []
self.dend_spine = []
#number of spikes per micron
spine_per_micron = 2
for e in self.dend:
if e.diam >= 0 and e.diam < 1.5:
self.dend_spine.append(e)
original_dend_index = []
for old_index, section_acess in enumerate(self.dend):
if section_acess.diam >= 0 and section_acess.diam < 1.5:
original_dend_index.append(old_index)
for local_index, x_dend in enumerate(self.dend_spine):
dend_index.append(local_index)
dend_local_len.append(int(x_dend.L) * spine_per_micron)
dend_len.append(int(x_dend.L) * spine_per_micron)
to_list = np.linspace(0.05, 0.95, num = int(x_dend.L) * spine_per_micron)
spine_location.append(to_list.tolist())
dend_total_len = sum(dend_len)
info_dend = list(zip(dend_index,dend_local_len))
info_dend_old_index = list(zip(original_dend_index,dend_local_len))
spine_number = int(dend_total_len)
somma_location_spines = sum(spine_location,[])
self.spine_head = [h.Section(name='spine_head_'+str(x)) for x in range(spine_number)]
for i in self.spine_head:
i.nseg = 1
i.diam = 1
i.cm = 2
i.L = 0.35
i.Ra = 122
i.insert('Leak')
i.e_Leak = -61
i.gmax_Leak = 1e-5
i.insert('Cav2_1')
i.pcabar_Cav2_1 = 1e-5
i.insert('Cav3_1')
i.pcabar_Cav3_1 = 1e-6
i.insert('Kca1_1')
i.gbar_Kca1_1 = 0.01
i.insert('Kca2_2')
i.gkbar_Kca2_2 = 1e-3
i.insert('Kv4_3')
i.gkbar_Kv4_3 = 0.005
i.insert('cdp5_CAM')
i.Nannuli_cdp5_CAM = 0.326 + (1.94 * (i.diam)) + (0.289*(i.diam)*(i.diam)) - ((3.33e-2)*(i.diam)*(i.diam)*(i.diam)) + ((1.55e-3)*(i.diam)*(i.diam)*(i.diam)*(i.diam)) - (2.55e-5*(i.diam)*(i.diam)*(i.diam)*(i.diam)*(i.diam))
i.Buffnull2_cdp5_CAM = 64.2 - 57.3* math.exp(-(i.diam)/1.4)
i.rf3_cdp5_CAM = 0.162 - 0.106* math.exp(-(i.diam)/2.29)
i.rf4_cdp5_CAM = 0.003
i.push()
h.ion_style("ca_ion", 1, 1, 0, 1, 0)
i.eca = 137.52625
i.cai = h.cai0_ca_ion
i.cao = h.cao0_ca_ion
h.pop_section()
i.TotalPump_cdp5_CAM = 7e-10
#NECK OF THE SPINE
self.spine_neck = [h.Section(name='spine_neck_'+str(x)) for x in range(spine_number)]
for x in self.spine_neck:
x.nseg = 1
x.diam = 0.2
x.cm = 3
x.L = 0.7
x.Ra = 122
x.insert('Leak')
x.e_Leak = -61
x.gmax_Leak = 1e-5
for i,j in enumerate(self.spine_head):
j.connect(self.spine_neck[i],1,0)
print("spine active!!")
list_dend_local = []
c,v = zip(*info_dend)
for i in range(len(info_dend)):
new_list = []
new_list = [c[i]]*v[i]
list_dend_local.append(new_list)
list_dend_old_index = []
c_old,v_old = zip(*info_dend_old_index)
for i in range(len(info_dend_old_index)):
new_list_oldindex = []
new_list_oldindex = [c_old[i]]*v_old[i]
list_dend_old_index.append(new_list_oldindex)
list_dend_complete = sum(list_dend_local,[])
list_dend_complete_oldindex = sum(list_dend_old_index,[])
self.total_list = list(zip(range(dend_total_len), list_dend_complete, somma_location_spines))
print('dend_total_len', len(range(dend_total_len)))
print('list_dend_complete', len(list_dend_complete))
print('somma_location_spines', len(somma_location_spines))
spine_sec_aa = []
spine_sec_pf = []
spine_sec_aa_SC = []
spine_print_list = range(0, len(self.total_list), 500)
c_source,v_dest,z_step = zip(*self.total_list) # outside the for... not inside....
for spines_created in range(len(self.total_list)):
if spines_created in spine_print_list:
print('spines_placed', spines_created)
#AA
if self.dend_spine[v_dest[spines_created]].diam <= 0.3:
self.spine_neck[c_source[spines_created]].connect(self.dend_spine[v_dest[spines_created]],z_step[spines_created],0)
spine_sec_aa.append(c_source[spines_created])
#AA/SC
if self.dend_spine[v_dest[spines_created]].diam > 0.3 and self.dend_spine[v_dest[spines_created]].diam <= 0.55:
self.spine_neck[c_source[spines_created]].connect(self.dend_spine[v_dest[spines_created]],z_step[spines_created],0)
spine_sec_aa_SC.append(c_source[spines_created])
#PF/SC
if self.dend_spine[v_dest[spines_created]].diam > 0.55 and self.dend_spine[v_dest[spines_created]].diam <= 1.4:
self.spine_neck[c_source[spines_created]].connect(self.dend_spine[v_dest[spines_created]],z_step[spines_created],0)
spine_sec_pf.append(c_source[spines_created])
#AA
self.spine_head_aa = []
#SC
self.spine_head_aa_SC = []
#PF
self.spine_head_pf = []
spine_print_list_syn = range(0, len(self.spine_head), 5000)
for i_head, e_head in enumerate(self.spine_head):
if i_head in spine_print_list_syn:
print('spines_syn: ', e_head)
elif i_head in spine_sec_aa:
self.spine_head_aa.append(e_head)
elif i_head in spine_sec_aa_SC:
self.spine_head_aa_SC.append(e_head)
elif i_head in spine_sec_pf:
self.spine_head_pf.append(e_head)
elif i_head in spine_sec_CF:
self.spine_head_CF.append(e_head)
print('spine_head_aa between 0 - 0.3 ->', len(self.spine_head_aa))
print('spine_head_aa_SC between 0.3 - 0.55 ->', len(self.spine_head_aa_SC))
print('spine_head_pf_SC between 0.55 - 1.4 ->', len(self.spine_head_pf))
print('!!!!!!!!!!!!!!! spine_total', len(self.spine_head_aa_SC) + len(self.spine_head_aa) + len(self.spine_head_pf), ' !!!!!!!!!!!!!!!')
self.dendAA_SC = []
self.dendAA_SC_index = []
self.dendPF_SC = []
self.dendPF_SC_index = []
for i,e in enumerate(self.dend):
if e.diam > 0.3 and e.diam < 0.55:
self.dendAA_SC.append(e)
self.dendAA_SC_index.append(i)
if e.diam > 0.55 and e.diam < 1.4:
self.dendPF_SC.append(e)
self.dendPF_SC_index.append(i)
#Recorder section.
self.time_vector = h.Vector()
self.time_vector.record(h._ref_t)
self.vm_soma = h.Vector()
self.vm_soma.record(self.soma[0](0.5)._ref_v)
def createsyn(self, npf, naa, numpfstl):
self.dendparallel = []
self.dendascending = []
self.dendAAstl = []
self.dendPFstl = []
#new subdivision for the synapses
for i in self.dend:
if i.diam > 0.55 and i.diam < 1.4:
self.dendparallel.append(i)
self.dendPFstl.append(i)
if i.diam <= 0.55:
self.dendascending.append(i)
print("PF")
print(len(self.dendparallel))
print("AA")
print(len(self.dendascending))
#Parallel fiber list
self.PF_L = []
#Ascending axon list
self.AA_L = []
#Stellate list
self.SC_L = []
#Randomizer
self.pfrand = []
self.aarand = []
self.stlrand = []
self.bgrand = []
self.pfstlrand = []
self.aastlrand = []
#New lists for the new stellate setup
self.pfSC_L = []
self.aaSC_L = []
self.cfSC_L = []
n = len(self.dendparallel)
n2 = len(self.dendascending)
npfstl = len(self.dendPFstl)
#PF random
for j in range(npf):
n -= 1
i = random.randint(0, n)
self.dendparallel[i], self.dendparallel[n] = self.dendparallel[n], self.dendparallel[i]
self.pfrand.append(self.dendparallel[n])
self.PF_L.append(Synapse_py3('pf',self,self.pfrand[j]))
#AA random
for j in range(naa):
n2 -= 1
i = random.randint(0, n2)
self.dendascending[i], self.dendascending[n2] = self.dendascending[n2], self.dendascending[i]
self.aarand.append(self.dendascending[n2])
self.AA_L.append(Synapse_py3('aa',self,self.aarand[j]))
#STl_PF random
for j in range(numpfstl):
npfstl -= 1
i = random.randint(0, npfstl)
self.dendPFstl[i], self.dendPFstl[npfstl] = self.dendPFstl[npfstl], self.dendPFstl[i]
self.stlrand.append(self.dendPFstl[npfstl])
self.SC_L.append(Synapse_py3('stl',self,self.stlrand[j]))
def dendrites_xy_nospine(self,min_x, max_x, min_y,max_y,syntype):#,option_random,number_random_syn):
self.dend_cood_x = {}
self.dend_cood_y = {}
for i, j in enumerate(self.dend):
j.push()
self.dend_cood_x["x0_"+str(i)] = h.x3d(0)
self.dend_cood_y["y0_"+str(i)] = h.y3d(0)
h.pop_section()
#only of X
dend_x_values = sorted(self.dend_cood_x.values()) #sorting of the values
dend_x_kyes = sorted(self.dend_cood_x, key=self.dend_cood_x.get) #sorting of the keys based on the values
dend_list_zip_x = zip(dend_x_kyes, dend_x_values)
#print 'dimensions X'
#print min_x
#print max_x
#only of Y
dend_y_values = sorted(self.dend_cood_y.values()) #sorting of the values
dend_y_kyes = sorted(self.dend_cood_y, key=self.dend_cood_y.get) #sorting of the keys based on the values
dend_list_zip_y = zip(dend_y_kyes, dend_y_values)
#print 'dimensions Y'
#print min_y
#print max_y
self.x_coord_dend = []
xlist = []
for i in dend_list_zip_x:
if i[1] > min_x and i[1] < max_x:
xlist.append(i[1])
self.x_coord_dend.append(int(i[0][3:]))
self.y_coord_dend = []
ylist = []
for i in dend_list_zip_y:
if i[1] > min_y and i[1] < max_y:
ylist.append(i[1])
self.y_coord_dend.append(int(i[0][3:]))
#print "dend number"
#print len(self.x_coord_dend)
#print len(self.y_coord_dend)
self.total_dend = []
self.total_dend = set(self.x_coord_dend).intersection(self.y_coord_dend)
#print 'number syn'
#print self.total_dend
print('dend set', self.total_dend)
print('Dend synapses ON.')
self.diam = []
for x in self.total_dend:
self.diam.append(self.dend[x].diam)
self.dend_diam = zip(self.total_dend, self.diam)
#new list of synapses
self.AAtotal = []
self.PFtotal = []
self.SCtotal = []
if syntype == 0:
self.AAdendminmax = []
if syntype == 1:
self.PFdendminmax = []
if syntype == 2:
self.SCdendminmax = []
for x in self.dend_diam:
if syntype == 0:
if x[1] <= 0.55:
#if option_random == 1:
#self.AAtotal.append(self.dend[x[0]])
#else:
self.AAdendminmax.append(Synapse_py3('aa',self,self.dend[x[0]]))
#print('AA')
if syntype == 1:
if x[1] > 0.55 and x[1] < 1.4:
#if option_random == 1:
#self.PFtotal.append(self.dend[x[0]])
##print('x[0]', x[0])
#else:
self.PFdendminmax.append(Synapse_py3('pf',self,self.dend[x[0]]))
#print('x[0]', x[0])
#print('nope')
if syntype == 2:
if x[1] >= 0.3 and x[1] < 1.4:
#if option_random == 1:
#self.SCtotal.append(self.dend[x[0]])
#else:
self.SCdendminmax.append(Synapse_py3('stl',self,self.dend[x[0]]))
if syntype == 0:
print('AA syn number!!!!!!!!!!!!!!!!!!!!!!!!!!!', len(self.AAdendminmax))
#self.AAdendminmax = self.AAdendminmax + self.AAdendminmax + self.AAdendminmax
elif syntype == 1:
print('PF syn number!!!!!!!!!!!!!!!!!!!!!!!!!!!', len(self.PFdendminmax))
#self.PFdendminmax = self.PFdendminmax + self.PFdendminmax + self.PFdendminmax
elif syntype == 2:
print('STL syn number!!!!!!!!!!!!!!!!!!!!!!!!!!', len(self.SCdendminmax))
#new spine coordinator
def spine_space_limit(self):
h.define_shape()
self.spinehead_coord_x = {}
self.spinehead_coord_y = {}
print('checking the size of the morphology')
for i, j in enumerate(self.spine_head):
j.push()
self.spinehead_coord_x["x0_"+str(i)] = h.x3d(0)
self.spinehead_coord_y["y0_"+str(i)] = h.y3d(0)
h.pop_section()
##only of X
self.spine_space_x = sorted(self.spinehead_coord_x.values())
##only of Y
self.spine_space_y = sorted(self.spinehead_coord_y.values())
def dendrites_xy(self,min_x, max_x, min_y,max_y):
self.dend_cood_x = {}
self.dend_cood_y = {}
for i, j in enumerate(self.dend):
j.push()
self.dend_cood_x["x0_"+str(i)] = h.x3d(0)
self.dend_cood_y["y0_"+str(i)] = h.y3d(0)
h.pop_section()
#only of X
dend_x_values = sorted(self.dend_cood_x.values())
dend_x_kyes = sorted(self.dend_cood_x, key=self.dend_cood_x.get)
dend_list_zip_x = zip(dend_x_kyes, dend_x_values)
#only of Y
dend_y_values = sorted(self.dend_cood_y.values())
dend_y_kyes = sorted(self.dend_cood_y, key=self.dend_cood_y.get)
dend_list_zip_y = zip(dend_y_kyes, dend_y_values)
self.x_coord_dend = []
xlist = []
for i in dend_list_zip_x:
if i[1] > min_x and i[1] < max_x:
xlist.append(i[1])
self.x_coord_dend.append(int(i[0][3:]))
self.y_coord_dend = []
ylist = []
for i in dend_list_zip_y:
if i[1] > min_y and i[1] < max_y:
ylist.append(i[1])
self.y_coord_dend.append(int(i[0][3:]))
self.total_spines = []
self.total_spines = set(self.x_coord_dend).intersection(self.y_coord_dend)
print('total_dend_SC', len(self.total_spines))
#New code dend only on X and Y at the same time to gnerate spots of activation
def spine_heads_x_y(self,min_x, max_x, min_y,max_y):
h.define_shape() #gives pt3d x,v,z to all the spines
self.spinehead_coord_x = {}
self.spinehead_coord_y = {}
for i, j in enumerate(self.spine_head):
j.push()
self.spinehead_coord_x["x0_"+str(i)] = h.x3d(0)
self.spinehead_coord_y["y0_"+str(i)] = h.y3d(0)
h.pop_section()
#only of X
spine_x_values = sorted(self.spinehead_coord_x.values()) #sorting of the values
spine_x_kyes = sorted(self.spinehead_coord_x, key=self.spinehead_coord_x.get) #sorting of the keys based on the values
spine_list_zip_x = zip(spine_x_kyes, spine_x_values)
#only of Y
spine_y_values = sorted(self.spinehead_coord_y.values()) #sorting of the values
spine_y_kyes = sorted(self.spinehead_coord_y, key=self.spinehead_coord_y.get) #sorting of the keys based on the values
spine_list_zip_y = zip(spine_y_kyes, spine_y_values)
self.x_coord_spinehead = []
xlist = []
for i in spine_list_zip_x:
if i[1] > min_x and i[1] < max_x:
xlist.append(i[1])
self.x_coord_spinehead.append(int(i[0][3:]))
self.y_coord_spinehead = []
ylist = []
for i in spine_list_zip_y:
if i[1] > min_y and i[1] < max_y:
ylist.append(i[1])
self.y_coord_spinehead.append(int(i[0][3:]))
self.total_spines = []
self.total_spines = set(self.x_coord_spinehead).intersection(self.y_coord_spinehead)
print('total_spines', len(self.total_spines))
def activator(self, syntype, option_random, n_aa_set, n_pf_set, n_aa_sc_set, n_pf_sc_set, n_aa_aa_set):
print('spine synapses ON.')
#AA only with random and fixed
self.AAdendminmax = []
self.aarand = []
self.aarand_spine_num = []
self.AA_total = []
if syntype == 0:
for sec_name_value_AA in self.spine_head_aa:
for y in self.total_spines:
if 'spine_head_' + str(y) == str(sec_name_value_AA):
if option_random == 0:
self.AAdendminmax.append(Synapse_py3('aa',self,sec_name_value_AA))
self.aarand_spine_num.append(self.sec_name_value_AA.name())
else:
self.AA_total.append(self.sec_name_value_AA)
if option_random == 1:
naa = len(self.AA_total)
for z in range(int((naa/100)*n_aa_set)):
naa -= 1
i = random.randint(0, naa)
self.AA_total[i], self.AA_total[naa] = self.AA_total[naa], self.AA_total[i]
self.aarand.append(self.AA_total[naa])
self.AAdendminmax.append(Synapse_py3('aa',self,self.self.aarand[z]))
self.aarand_spine_num.append(self.aarand[z].name())
#PF only with random and fixed
self.PFdendminmax = []
self.pfrand = []
self.pfrand_spine_num = []
self.PF_total = []
if syntype == 1:
for sec_name_value_PF in self.spine_head_pf:
for y in self.total_spines:
if 'spine_head_' + str(y) == str(sec_name_value_PF):
if option_random == 0:
self.PFdendminmax.append(Synapse_py3('pf',self,sec_name_value_PF))
self.pfrand_spine_num.append(sec_name_value_PF.name())
else:
self.PF_total.append(sec_name_value_PF)
if option_random == 1:
npf = len(self.PF_total)
for z in range(int((npf/100)*n_pf_set)):
npf -= 1
i = random.randint(0, npf)
self.PF_total[i], self.PF_total[npf] = self.PF_total[npf], self.PF_total[i]
self.pfrand.append(self.PF_total[npf])
self.PFdendminmax.append(Synapse_py3('pf',self,self.pfrand[z]))
self.pfrand_spine_num.append(self.pfrand[z].name())
#SC on AA only with random and fixed
self.SC_AAdendminmax = []
self.SC_AA_rand = []
self.SC_AA_spine_num = []
self.SC_AA_total = []
if syntype == 2:
for sec_name_value_SC_AA in self.dendAA_SC_index:
for y in self.total_spines:
if str(y) == str(sec_name_value_SC_AA):
if option_random == 0:
self.SC_AAdendminmax.append(Synapse_py3('stl',self,self.dend[sec_name_value_SC_AA]))
self.SC_AA_spine_num.append(sec_name_value_SC_AA)
else:
self.SC_AA_total.append(self.dend[sec_name_value_SC_PF])
if option_random == 1:
naasc = len(self.SC_AA_total)
for z in range(int((naasc/100)*n_aa_sc_set)):
naasc -= 1
i = random.randint(0, naasc)
self.SC_AA_total[i], self.SC_AA_total[naasc] = self.SC_AA_total[naasc], self.SC_AA_total[i]
self.SC_AA_rand.append(self.SC_AA_total[naasc])
self.SC_AAdendminmax.append(Synapse_py3('stl',self,self.SC_AA_rand[z]))
self.SC_AA_spine_num.append(self.SC_AA_rand[z].name())
#SC on PF only with random and fixed
self.SC_PFdendminmax = []
self.SC_PF_rand = []
self.SC_PF_spine_num = []
self.SC_PF_total = []
if syntype == 3:
for sec_name_value_SC_PF in self.dendPF_SC_index:
for y in self.total_spines:
if y == sec_name_value_SC_PF:
if option_random == 0:
#print('no random SC_PF')
self.SC_PFdendminmax.append(Synapse_py3('stl',self,self.dend[sec_name_value_SC_PF]))
self.SC_PF_spine_num.append(sec_name_value_SC_PF)
else:
self.SC_PF_total.append(self.dend[sec_name_value_SC_PF])
if option_random == 1:
naapf = len(self.SC_PF_total)
for z in range(int((naapf/100)*n_pf_sc_set)):
naapf -= 1
i = random.randint(0, naapf)
self.SC_PF_total[i], self.SC_PF_total[naapf] = self.SC_PF_total[naapf], self.SC_PF_total[i]
self.SC_PF_rand.append(self.SC_PF_total[naapf])
self.SC_PFdendminmax.append(Synapse_py3('stl',self,self.SC_PF_rand[z]))
self.SC_PF_spine_num.append(self.SC_PF_rand[z].name())
#AA only in SC location
self.AA_AAdendminmax = []
self.AA_AA_rand = []
self.AA_AA_spine_num = []
self.AA_AA_total = []
if syntype == 4:
for sec_name_value_AA_AA in self.spine_head_aa_SC:
for y in self.total_spines:
if 'spine_head_' + str(y) == str(sec_name_value_AA_AA):
if option_random == 0:
self.AA_AAdendminmax.append(Synapse_py3('aa',self,sec_name_value_AA_AA))
self.AA_AA_spine_num.append(sec_name_value_AA_AA.name())
else:
self.AA_AA_total.append(sec_name_value_AA_AA)
if option_random == 1:
naa_aasc = len(self.AA_AA_total)
for z in range(int((naa_aasc/100)*n_aa_aa_set)):
naa_aasc -= 1
i = random.randint(0, naa_aasc)
self.AA_AA_total[i], self.AA_AA_total[naa_aasc] = self.AA_AA_total[naa_aasc], self.AA_AA_total[i]
self.AA_AA_rand.append(self.AA_AA_total[naa_aasc])
self.AA_AAdendminmax.append(Synapse_py3('aa',self,self.AA_AA_rand[z]))
self.AA_AA_spine_num.append(self.AA_AA_rand[z].name())