README_R15.txt The goal of the present simulation is to illustrate how SNNAP can be used to simulation the biophysical properties of a relatively complex neuron. The R15 model is based on models originally published by Canavier et al. (1991) and Butera et al. 1995: Butera, R.J., Clark, J.W., Canavier, C.C., Baxter, D.A., and Byrne, J.H. (1995) Analysis of the effects of modulatory agents on a modeled bursting neuron: dynamic interations between voltage and calcium dependent systems. J. Comput. Neurosci. 2: 19-44. Canavier, C.C., Clark, J.W. and Byrne, J.H. (1991) Simulation of the bursting activity of neuron R15 in Aplysia: role of ionic currents, calcium balance, and modulatory transmitters. J. Neurophysiol. 66: 2107-2124. The components of the R15 neuron are illustrated in R15_neu.jpg and Butera_model.jpg. The model contains eight ionic conductances, an intracellular pool of calcium (i.e., Ca.ion), an intracellular pool of second messenger (i.e., cAMP.sm), and modulatory interactions from the ion and second messenger pools and the membrane conductances. The results of the default simulation are illustrated in R15_ous.jpg. The model produces endogenous bursting activity. At time=60sec a modulator is applies via the treatment file (i.e., R15.trt). The resultant increase in the second messenger and modulation of membrane conductances alters the electrical activity of the neuron.