Readme for the models associated with: Bui, Tuan V., Diane E. Dewey, Robert E. W. Fyffe, and P. Ken Rose (2005) Comparison of the Inhibition of Renshaw Cells During Subthreshold and Suprathreshold Conditions Using Anatomically and Physiologically Realistic Models J Neurophysiol 94: 1688-1698, 2005. Inhibitory synaptic inputs to Renshaw cells are concentrated on the soma and the juxtasomatic dendrites. In the present study, we investigated whether this proximal bias leads to more effective inhibition under different neuronal operating conditions. Using compartmental models based on detailed anatomical measurements of intracellularly stained Renshaw cells, we compared the inhibition produced by glycine/gamma-aminobutyric acid-A (GABAA) synapses when distributed with a proximal bias to the inhibition produced when the same synapses were distributed uniformly (i.e., with no regional bias). The comparison was conducted in subthreshold and suprathreshold conditions. The latter were mimicked by voltage clamping the soma to -55 mV. The voltage clamp reduces nonlinear interactions between excitatory and inhibitory synapses. We hypothesized that for electrotonically compact cells such as Renshaw cells, the strength of the inhibition would become much less dependent on synaptic location in suprathreshold conditions. This hypothesis was not confirmed. The inhibition produced when inhibitory inputs were proximally distributed was always stronger than when the same inputs were uniformly distributed. In fact, the relative effectiveness of proximally distributed inhibitory inputs over uniformly distributed synapses was greater in suprathreshold conditions than that in subthreshold conditions. The somatic voltage clamp minimized saturation of inhibitory driving potentials. Because this effect was greatest near the soma, the current produced by more distal synapses suffered a greater loss because of saturation. Conversely, in subthreshold conditions, the effectiveness of proximal synapses was substantially reduced at high levels of background synaptic activity because of saturation. Our results suggest glycine/GABAA synapses on Renshaw cells are strategically distributed to block the powerful excitatory drive produced by recurrent collaterals from motoneurons. Four models for Renshaw cells (RC1a, RC2a, RC3a, and RC4a) and three models for neck motoneurons (LAD5-4, LVN2-1 and LVN4-1). The models include a main template (starts with the name of the cell followed by .sin). Within each of these main templates is a listing of the template for each primary subtree. In the template of each primary subtree is the listing of the membrane compartments that make up the primary subtree. The Renshaw cell models have glycinergic/GABAergic synapses and cholinergic synapses in the distribution that we described in Bui et al. (2005a) J. Neurophys. The Renshaw cell models were produced by Dr. Robert Fyffe, Dianne Dewey, Dr. Ken Rose and Tuan Bui, while the motoneurons models were produced by Dr. Ken Rose and Tuan Bui. These model files were contributed to ModelDB by Tuan Bui. Tuan V. Bui Queen's Univ Dept Physiol Botterell Hall Kingston ON K7L 3N6 Canada Work Phone: 613-533-6000 ext.74853 Fax: 613-533-6840 E-mail Address: tuan@biomed.queensu.ca