//genesis // PSim_Params.g /*************************** MS Model, Version 12 ***************/ //*********** includes - functions for constructing model and outputs, and doing simulations include SimParams.g //simulation control parameters, can be overridden in this file simdt=5e-6//2e-5 //outputclock=1e-4 spinesYesNo=1 synYesNo=1 delay= 0.2 duration = 0.4 //****** Globals and Parameters that can be changed in this script for the simulation pfile="MScell/MScelltaperspines.p"//"MScell/MScellPrimSecSpines.p"//"MScell/MScelltaperspines.p"//"MScell/MScelltaperspines_SingleBranchNarrow.p" //Simplified morphology for tuning str comps="soma,primdend1,secdend11,tertdend1_1,tertdend1_2,tertdend1_3,tertdend1_4,tertdend1_5,tertdend1_6,tertdend1_7,tertdend1_8,tertdend1_9,tertdend1_10,tertdend1_11" //These should include comps of stim spines to see NMDA str chans="CaL12_channel,CaL13_channel,CaR_channel,CaN_channel,CaR_channel,CaT_channel,BK_channel,KIR_channel,KAf_channel,NaF_channel,SK_channel,NaFd_channel,KAs_channel,Krp_channel" include MScell/globals.g //change below //ELEAK = -.071//-.077 best//-.072//ELEAK*.4985//{ELEAK*.3*1.667}//-.075//-0.029478 //RA = 1.5//2.0//RA*7.199//{RA*6.9*1.67}//1.5//13.7657 //RM = 1.5//1.5= better IF//1.05//1.05 is best so far//.6//.5//0.8//0.5//0.75//1.0//10.0//RM*.5503//2//{RM*0.99*0.759}//.4//.1//.5//.2//8.97763 //CM = .005//.01//2*CM*.2734//{CM*0.5*0.95*4}//0.035 EREST_ACT = -.0835 //float qfactorKir = 1.2//1.2 //gNaFsoma_UI = 0 gNaFprox_UI =2500 gNaFdist_UI = 450 //gNaFsoma_UI=44000//{40000} //50000 //gNaFprox_UI={2730} //gNaFdist_UI={975} gCaL13soma_UI = {{2*3e-7}/{GHKluge}} gCaL13dend_UI = {{.5*0.5e-8}/{GHKluge}} //gCaTprox = {{0e-8}/{GHKluge}} gCaTdist = {{.5*8e-8}/{GHKluge}} gCaRsoma = {{2*8e-7}/{GHKluge}} gCaRdend ={{.5*10e-7}/{GHKluge}} gCaNsoma = {{2*12e-7}/{GHKluge}} //gCaNdend = {{0}/{GHKluge}} gCaL12soma_UI = {{2*6e-7}/{GHKluge}} gCaL12dend_UI = {{.5*1*1e-7}/{GHKluge}} //gKIRsoma_UI = 9.5//9 best//14//gKIRsoma_UI*.6826//{gKIRsoma_UI*.67*.867}// {10.0} //25.0658 //gKIRdend_UI = 0//9.5//10//10=best//14//20//gKIRsoma_UI//{15} //gKAfsoma_UI=217//190//170//100//160= good//50//25//50//100//315//250//{200} //gKAfdend_UI=0//gKAfsoma_UI//{90} //gKAssoma_UI=12//9//40//100//100//25//{100} //gKAsdend_UI=0//gKAssoma_UI//{10} //gKrpsoma =5//6//6 //gKrpdend=0//gKrpsoma//3 //6 //gBKdend =0//2// 5//5 //gBKsoma = 3//10//10 //20 //gSKdend = 0//1//1 //gSKsoma = 2//2//3 //qfactorkAs=2//3//9//2 //qfactorNaF = 2.5 //qfactorKrp = 3 //qfactorkAf=2.0//1.5 orig//2//3//2 include MScell/Ca_constants.g calciumdye = 0//2 calciuminact = 1 calciumtype=0 if (calciumdye == 0) btotal2 = 15.0e-3 //was 30.0e-3, but Rodrigo's paper seems to have about half the CaM as Myungs. else btotal2 = 0.0e-3 //CaM is 'dialyzed' when there is a calcium dye present end if (calciumdye == 0) btotal4 = 15.0e-3 //was 30.0e-3, but Rodrigo's paper seems to have about half the CaM as Myungs. else btotal4 = 0.0e-3 //CaM is 'dialyzed' when there is a calcium dye present end if (calciumdye==1) btotalfluor={btotal3} kffluor={kf3} kbfluor={kb3} bnamefluor={bname3} dfluor={d3} elif (calciumdye==2) btotalfluor={btotal5} kffluor={kf5} kbfluor={kb5} bnamefluor={bname5} dfluor={d5} end include MScell/SynParamsCtx.g AMPAgmax=1.0e-9//1.7e-9 //0.3e-9 N2Aratio=1.0//1.0 NMDAgmax = {AMPAgmax}*{N2Aratio} float Kmg = 18 //18 new //3.57 old overwrites 1/eta in nmda_channel.g include MScell/spineParams.g //spineRa= 0.5e15//0.5e9 spineRM = {RM} //10 //spineRA = {RA}//1//1 //gCaL12spine = 0//{getglobal gCaL12dend_{DA}} //3.35e-7 //gCaL13spine = 0//{getglobal gCaL13dend_{DA}} //4.25e-7 //gCaRspine = 0//{gCaRdend} //13e-7 gCaTspine = {gCaTdist} //0.235e-7 spineDensity=1.12//1.12//0.8//1//0.3 //in units of per micron