// In use 10/10/2004 -- present // Add striatal synapses, but normalize rate by compartment surface area. /* The following params must be set prior to calling this file: str striatumfname: file name of compartments to get striatal inputs int num_striatum_compts: number of compartments to get striatal inputs int num_striatum_per_comp: # striatal syns per selected compartment. All are currently initialized and set in GP$1_defaults.g, but can be overwritten as needed. Modified 10/10/2004: made the rate of each compartment's striatal inputs normalized by that compartment's surface area relative to the mean surface area for all compartments receiving striatal input. So the total number of events arriving at the "average" compartment = {striatum_rate} * {num_striatum_per_comp} This input rate can be translated to a constant, uniform input in units of events / sec / micron2 by dividing {striatum_rate} * {num_striatum_per_comp} by the total surface area of the dendritic compartments receiving input. */ if ({snapgate}==0) if ({synBack_synch}==0) randseed {({rseed_striatum_postsnap}+{spikeIdx})} echo echo post snapshot random seed is {({rseed_striatum_postsnap}+{spikeIdx})} echo end else // randseed {rseed_striatum} NWS: I think this is a mistake, there is no rseed_striatum in simdefaults randseed {rseed_Str} echo echo snapshot random seed is {rseed_Str} echo end int i float d,l,surf float totsurf, meansurf, thisrate str striatumcompartment //create input element tree outside of the cell path if (!{exists /inputs}) create neutral /inputs end create neutral /inputs/striatum // get sum total surface area of all compartments receiving striatal input totsurf = 0 openfile {striatumfname} r for (i = 1; i <= {num_striatum_compts}; i = i + 1) striatumcompartment = {readfile {striatumfname}} // get compt params d = {getfield {cellpath}/{striatumcompartment} dia} l = {getfield {cellpath}/{striatumcompartment} len} surf = {PI}*{l}*{d} totsurf = {totsurf} + {surf} end closefile {striatumfname} meansurf = {totsurf}/{num_striatum_compts} echo "Total dendritic surface area (microns2): " {{totsurf}*1e12} echo "Mean surface area of dendritic compartments (microns2): " {{meansurf}*1e12} echo "Total number of striatal input events per second: " {{striatum_rate}*{num_striatum_per_comp}*{num_striatum_compts}} echo "Striatal events per second for average compartment: " {{striatum_rate}*{num_striatum_per_comp}} echo "Striatal events per second per square micron: " {{striatum_rate}*{num_striatum_per_comp}*{num_striatum_compts}/({totsurf}*1e12)} //cycle through each selected compartment and add synapses openfile {striatumfname} r //openfile "rates_GABA.asc" w echo echo G_GABA is {G_GABA} echo echo syngain is {syngain} echo for (i = 1; i <= {num_striatum_compts}; i = i + 1) striatumcompartment = {readfile {striatumfname}} // get compt params d = {getfield {cellpath}/{striatumcompartment} dia} l = {getfield {cellpath}/{striatumcompartment} len} surf = {PI}*{l}*{d} copy /library/GABA {cellpath}/{striatumcompartment}/GABA addmsg {cellpath}/{striatumcompartment}/GABA \ {cellpath}/{striatumcompartment} CHANNEL Gk Ek addmsg {cellpath}/{striatumcompartment} \ {cellpath}/{striatumcompartment}/GABA VOLTAGE Vm //echo //echo striatal gmax before is {getfield {cellpath}/{striatumcompartment}/GABA gmax} setfield {cellpath}/{striatumcompartment}/GABA \ gmax {{G_GABA}*{syngain}} //echo striatal gmax after is {getfield {cellpath}/{striatumcompartment}/GABA gmax} //echo //set up timetables with rates scaled by compartment surface area create neutral /inputs/striatum/{striatumcompartment} create timetable /inputs/striatum/{striatumcompartment}/timetable if ({striatum_rate} > 0) thisrate = {striatum_rate} * {surf} / {meansurf} // writefile "rates_GABA.asc" {thisrate} setfield /inputs/striatum/{striatumcompartment}/timetable \ maxtime 20 act_val 1.0 method 2 \ meth_desc1 {1/{{thisrate}*{num_striatum_per_comp}}} \ meth_desc2 0 meth_desc3 3 call /inputs/striatum/{striatumcompartment}/timetable TABFILL end //set up spikegen create spikegen /inputs/striatum/{striatumcompartment}/spikegen setfield /inputs/striatum/{striatumcompartment}/spikegen \ output_amp 1 thresh 0.5 //connect timetables to GABA synapses if ({striatum_rate} > 0) addmsg /inputs/striatum/{striatumcompartment}/timetable \ /inputs/striatum/{striatumcompartment}/spikegen INPUT activation addmsg /inputs/striatum/{striatumcompartment}/spikegen \ {cellpath}/{striatumcompartment}/GABA SPIKE end end closefile {striatumfname} //closefile "rates_GABA.asc"