/********************************************************************
section list of all apical dendrites
*************************************************/
objref all_apicals
all_apicals = new SectionList()
dendA5_0 all_apicals.subtree()
/********************************************************************
section list of all basal dendrites
*************************************************/
objref all_basals
all_basals = new SectionList()
dendA1_0 all_basals.subtree()
dendA2_0 all_basals.subtree()
dendA3_0 all_basals.subtree()
dendA4_0 all_basals.subtree()
/********************************************************************
section list of primary apical branch
*************************************************/
objref primary_apical_list
primary_apical_list = new SectionList()
dendA5_0 primary_apical_list.append
dendA5_01 primary_apical_list.append
dendA5_011 primary_apical_list.append
dendA5_0111 primary_apical_list.append
dendA5_01111 primary_apical_list.append
dendA5_011111 primary_apical_list.append
dendA5_0111111 primary_apical_list.append
dendA5_01111111 primary_apical_list.append
dendA5_011111111 primary_apical_list.append
dendA5_0111111111 primary_apical_list.append
dendA5_01111111111 primary_apical_list.append
dendA5_011111111111 primary_apical_list.append
dendA5_0111111111111 primary_apical_list.append
dendA5_01111111111111 primary_apical_list.append
dendA5_011111111111111 primary_apical_list.append
dendA5_0111111111111111 primary_apical_list.append
dendA5_01111111111111111 primary_apical_list.append
dendA5_011111111111111111 primary_apical_list.append
dendA5_0111111111111111111 primary_apical_list.append
/* reset nseg in primary apical so none smaller than 5 microns */
forsec primary_apical_list { ns=int(L/5+0.5)
if (ns==0) {
ns=1
}
if ((ns-int(ns/2)*2)==0) {
ns=ns+1
}
nseg = ns
}
/********************************************************************
section list of the spine
*************************************************/
objref all_spines
all_spines = new SectionList()
spine_head all_spines.append
spine_neck all_spines.append
/********************************************************************
initialize basic parameters
*************************************************/
celsius = 30 /* temperature */
v_init=-65
global_ra=150.00 /* internal resistivity in ohm-cm */
Cm=0.75 /* specific membrane capacitance in uF/cm^2 */
Rm=40000 /* specific membrane resistivity in ohm-cm^2 */
Vleak=-65 /* leak reversal -65mV */
Vrest=-65 /* resting potential -64.6 mV*/
spinelimit=100 /* distance beyond which to modify for spines */
spinefactor=2.0 /* factor by which to change passive properties */
isegfactor=100
isegfrac=0.8
/********************************************************************
procedure to insert and initialize channels
*************************************************/
proc initchannels(){
/* passive properties */
print "Inserting passive membrane properties"
forall {insert pas g_pas=1/(Rm) Ra=global_ra e_pas=Vleak
}
/* soma active properties */
somaA {insert pas e_pas=Vleak g_pas=1/Rm Ra=global_ra cm=Cm
}
/* basal dendrites active properties */
forsec all_basals {insert pas e_pas=Vleak g_pas=1/Rm Ra=global_ra cm=Cm
}
spine_head {
insert pas e_pas=Vleak g_pas=spinefactor/Rm Ra=global_ra cm=spinefactor*Cm
insert car
insert cadiffus
insert canmda
}
spine_neck{
insert pas e_pas=Vleak g_pas=spinefactor/Rm Ra=global_ra cm=spinefactor*Cm
insert cadiffus
}
dendA5_00{
insert cadiffus
}
access somaA
area(0.5)
distance()
/* procedure to insert channels and set parameters in apical dendrites */
forsec all_apicals {
/* do passive properties; modify for spines if far enough out */
insert pas e_pas=Vleak Ra=global_ra
for (x) { xdist=distance(x)
if (xdist<=spinelimit) {
g_pas(x)=1/Rm
cm(x)=Cm
} else {
g_pas(x)=spinefactor/Rm
cm(x)=spinefactor*Cm
}
}
}
}
print "Passive properties and channels are initialized"