proc initial_values(){
dend_na12 =0.0001/2
dend_k = 0.004226
soma_na12 = 0.983955/2
soma_K = 0.303472
node_na = 2
axon_KP =0.973538
axon_KT = 0.089259
axon_K = 1.021945
ais_na16 = 7 //3.137968
ais_na12 = 5 //3.137968
ais_ca = 0.000990
ais_KCa = 0.007104
soma_na16 = soma_na12
naked_axon_na = soma_na16/5
navshift = -10
dend_na16 =dend_na12
myelin_na = naked_axon_na
myelin_K = 0.303472
myelin_scale = 10
gpas_all = 3e-5
cm_all = 1
}
// modify the axon for Roy's purposes (whole thing)
proc populate_axon(){
forsec cell.axonal{
gNaTa_tbar_NaTa_t = 0
gNap_Et2bar_Nap_Et2 = 0
gCa_HVAbar_Ca_HVA = 0
gSK_E2bar_SK_E2 = 0
nseg=11
g_pas(0:0.1) = (gpas_all/myelin_scale):(gpas_all/myelin_scale)
g_pas(0.1:1) = gpas_all:gpas_all
cm(0:0.1) = (cm_all/myelin_scale):(cm_all/myelin_scale)
cm(0.1:1) = cm_all:cm_all
gbar_na16(0:0.1) = node_na:node_na
gbar_na16(0.1:1) = myelin_na:myelin_na
gSKv3_1bar_SKv3_1(0:0.1) = axon_K:axon_K
gSKv3_1bar_SKv3_1(0.1:1) = myelin_K:myelin_K
gK_Pstbar_K_Pst(0:0.1) = axon_KP:axon_KP
gK_Pstbar_K_Pst(0.1:1) = axon_KP/10:axon_KP/10
gK_Tstbar_K_Tst(0:0.1) = axon_KT:axon_KT
gK_Tstbar_K_Tst(0.1:1) = axon_KT/10:axon_KT/10
}
}
proc populate_axon_young(){
forsec cell.axonal{
gbar_na16 = 0
insert na1216
insert na1216mut
gNaTa_tbar_NaTa_t = 0
gNap_Et2bar_Nap_Et2 = 0
gCa_HVAbar_Ca_HVA = 0
gSK_E2bar_SK_E2 = 0
nseg=11
g_pas(0:0.1) = (gpas_all/myelin_scale):(gpas_all/myelin_scale)
g_pas(0.1:1) = gpas_all:gpas_all
cm(0:0.1) = (cm_all/myelin_scale):(cm_all/myelin_scale)
cm(0.1:1) = cm_all:cm_all
gbar_na1216mut(0:0.1) = node_na/2:node_na/2
gbar_na1216(0:0.1) = node_na/2:node_na/2
gbar_na1216(0.1:1) = myelin_na/2:myelin_na/2
gbar_na1216mut(0.1:1) = myelin_na/2:myelin_na/2
gSKv3_1bar_SKv3_1(0:0.1) = axon_K:axon_K
gSKv3_1bar_SKv3_1(0.1:1) = myelin_K:myelin_K
gK_Pstbar_K_Pst(0:0.1) = axon_KP:axon_KP
gK_Pstbar_K_Pst(0.1:1) = axon_KP/10:axon_KP/10
gK_Tstbar_K_Tst(0:0.1) = axon_KT:axon_KT
gK_Tstbar_K_Tst(0.1:1) = axon_KT/10:axon_KT/10
}
}
// Specifically modify the first segment to create an AIS
proc create_ais(){
access cell.axon[0]
gNaTa_tbar_NaTa_t = 0 // Get rid of old sodium channels
gNap_Et2bar_Nap_Et2 = 0 // Get rid of old sodium channels
nseg = int(L/10)*2 +1 // create 19 segments from this axon section
ais_end = 10/nseg // specify the end of the AIS as halfway down this section
ais_mid = 4/nseg // specify the middle of the AIS as 1/5 of this section
gbar_na16(0:ais_mid) = 0:ais_na16 // from the start to mid of AIS, create gradient of NaV1.6
gbar_na16(ais_mid:ais_end) = ais_na16:ais_na16 // add consistent amount NaV1.6 until the end of the AIS
gbar_na16(ais_end:1) = naked_axon_na:naked_axon_na // 1/5th nav1.6
gbar_na12(0:ais_mid/2) = soma_na12/2:ais_na12/2 // create gradient of NaV12 from [soma] to [AIS]
gbar_na12mut(0:ais_mid/2) = soma_na12/2:ais_na12/2 // add a mutant channel in case we want to do a het
gbar_na12(ais_mid/2:ais_mid) = ais_na12/2:0 // create gradient down to zero
gbar_na12mut(ais_mid/2:ais_mid) = ais_na12/2:0 // more mutant channel
gbar_na12(ais_mid:1) = 0:0 //naked axon
gbar_na12mut(ais_mid:1) = 0:0 //naked axon
gK_Pstbar_K_Pst(0:ais_end) = axon_KP:axon_KP
gK_Pstbar_K_Pst(ais_end:1) = axon_KP/10:axon_KP/10
gK_Tstbar_K_Tst(0:ais_end) = axon_KT:axon_KT
gK_Tstbar_K_Tst(ais_end:1) = axon_KT/10:axon_KT/10
gSKv3_1bar_SKv3_1(0:ais_end) = axon_K:axon_K
gSKv3_1bar_SKv3_1(ais_end:1) = axon_K/10:axon_K/10
gCa_HVAbar_Ca_HVA(0:ais_end) = ais_ca:ais_ca
gCa_HVAbar_Ca_HVA(0:ais_end) = ais_ca/10:ais_ca/10
gSK_E2bar_SK_E2(0:ais_end) = ais_KCa:ais_KCa // SK channel
gSK_E2bar_SK_E2(ais_end:1) = ais_KCa/10:ais_KCa/10
access cell.soma
}
proc create_ais_young(){
access cell.axon[0]
gbar_na16 = 0
gNaTa_tbar_NaTa_t = 0
gNap_Et2bar_Nap_Et2 = 0
nseg = int(L/10)*2 +1
ais_end = 10/nseg
ais_mid = 4/nseg
//Replacing 16 with 1/2 12 and half 12mut
gbar_na1216(0:ais_mid) = 0:ais_na16/2
gbar_na1216mut(0:ais_mid) = 0:ais_na16/2
gbar_na1216(ais_mid:ais_end) = ais_na16/2:ais_na16/2
gbar_na1216mut(ais_mid:ais_end) = ais_na16/2:ais_na16/2
gbar_na1216(ais_end:1) = naked_axon_na/2:naked_axon_na/2 //naked axon
gbar_na1216mut(ais_end:1) = naked_axon_na/2:naked_axon_na/2 //naked axon
//
gbar_na12(0:ais_mid/2) = soma_na12/2:ais_na12/2
gbar_na12mut(0:ais_mid/2) = soma_na12/2:ais_na12/2
gbar_na12(ais_mid/2:ais_mid) = ais_na12/2:0
gbar_na12mut(ais_mid/2:ais_mid) = ais_na12/2:0
gbar_na12(ais_mid:1) = 0:0 //naked axon
gbar_na12mut(ais_mid:1) = 0:0 //naked axon
gK_Pstbar_K_Pst(0:ais_end) = axon_KP:axon_KP
gK_Pstbar_K_Pst(ais_end:1) = axon_KP/10:axon_KP/10
gK_Tstbar_K_Tst(0:ais_end) = axon_KT:axon_KT
gK_Tstbar_K_Tst(ais_end:1) = axon_KT/10:axon_KT/10
gSKv3_1bar_SKv3_1(0:ais_end) = axon_K:axon_K
gSKv3_1bar_SKv3_1(ais_end:1) = axon_K/10:axon_K/10
gCa_HVAbar_Ca_HVA(0:ais_end) = ais_ca:ais_ca
gCa_HVAbar_Ca_HVA(0:ais_end) = ais_ca/10:ais_ca/10
gSK_E2bar_SK_E2(0:ais_end) = ais_KCa:ais_KCa
gSK_E2bar_SK_E2(ais_end:1) = ais_KCa/10:ais_KCa/10
access cell.soma
}
proc update_soma_na(){
access cell.soma
vShift_na = navshift
gbar_na12 = soma_na12/2
gbar_na12mut = soma_na12/2
gbar_na16 = soma_na16
gNaTs2_tbar_NaTs2_t = 0
}
proc update_soma_na_young(){
access cell.soma
insert na1216
insert na1216mut
gbar_na16 = 0
vShift_na = navshift
gbar_na12 = soma_na12/2
gbar_na12mut = soma_na12/2
gbar_na1216 = soma_na16/2
gbar_na1216mut = soma_na16/2
gNaTs2_tbar_NaTs2_t = 0
}
proc update_dend_na(){
forsec cell.apical{
gbar_na12 = dend_na12/2
gbar_na12mut = dend_na12/2
gbar_na16 = dend_na16
gNaTs2_tbar_NaTs2_t = 0
}
}
proc update_dend_na_young(){
forsec cell.apical{
insert na1216
insert na1216mut
gbar_na12 = dend_na12/2
gbar_na16 = 0
gbar_na12mut = dend_na12/2
gNaTs2_tbar_NaTs2_t = 0
gbar_na1216 = dend_na16/2
gbar_na1216mut = dend_na16/2
}
}
proc working(){
populate_axon()
create_ais()
update_soma_na()
update_dend_na()
}
proc working_young(){
populate_axon_young()
create_ais_young()
update_soma_na_young()
update_dend_na_young()
}
proc run_gui(){
xpanel("params_values")
xvalue("ais_na12",&ais_na12 )
xvalue("ais_na16",&ais_na16)
xvalue("node_na",&node_na)
xvalue("soma_na16",&soma_na16)
xvalue("na_soma",&navshift)
xbutton("create_ais()")
xbutton("populate_axon()")
xbutton("update_soma_na()")
//xbutton("printVs()")
xpanel()
}
//run_gui()
initial_values()
access cell.axon[0]
L=90
access cell.soma
working()