package swcparts;
import java.io.*;
import java.text.*;
import java.math.*;
public class SwcNeuron {
private int maxSWClines = 100000;
private int maxCommentLines = 50000;
private String[] CommentLineArray = new String[maxCommentLines];
private SwcLine[] LineArray = new SwcLine[maxSWClines];
private int NeuronSize;
private int CommentSize;
private String FileName;
private String PatternOut = "0.####";
private DecimalFormat myFormatter = new DecimalFormat(PatternOut);
private boolean termDegreeSet = false;
public void InitSwcNeuron(String in) {
FileName = in;
try {
//Buffer input stream
//open input file
FileInputStream fin = new FileInputStream(in);
BufferedInputStream bin = new BufferedInputStream(fin);
//character stream
BufferedReader r = new BufferedReader(new InputStreamReader(bin));
r.mark(1);
//Input comment lines into string array
for (int i = 1; 1 < maxCommentLines; i++) {
CommentLineArray[i] = new String();
CommentLineArray[i] = r.readLine();
if ( (CommentLineArray[i] != null) && (CommentLineArray[i].startsWith("#"))) {
++CommentSize;
}
else {
break;
}
}
//Tokenize buffered input stream
r.reset();
StreamTokenizer tokens = new StreamTokenizer(r);
//set tokens paramaters
tokens.commentChar('#');
tokens.parseNumbers();
//initialize temp paramaters to read from swc file and place into SwcLines array
int tempLineNum;
int tempLineType;
double tempX;
double tempY;
double tempZ;
double tempRad;
int tempLink = 0;
boolean eof = false;
boolean eol = false;
int linesRead = 0;
int c;
int d;
tokens.eolIsSignificant(true);
while (eof == false) {
eol = false;
c = tokens.nextToken();
if (c == StreamTokenizer.TT_EOF) {
eof = true;
}
else if (c == StreamTokenizer.TT_EOL) {
eol = true;
}
else {
tempLineNum = (int) tokens.nval;
c = tokens.nextToken();
if (c == StreamTokenizer.TT_EOF) {
eof = true;
}
else if (c == StreamTokenizer.TT_EOL) {
eol = true;
}
else {
tempLineType = (int) tokens.nval;
c = tokens.nextToken();
if (c == StreamTokenizer.TT_EOF) {
eof = true;
}
else if (c == StreamTokenizer.TT_EOL) {
eol = true;
}
else {
tempX = tokens.nval;
c = tokens.nextToken();
if (c == StreamTokenizer.TT_EOF) {
eof = true;
}
else if (c == StreamTokenizer.TT_EOL) {
eol = true;
}
else {
tempY = tokens.nval;
c = tokens.nextToken();
if (c == StreamTokenizer.TT_EOF) {
eof = true;
}
else if (c == StreamTokenizer.TT_EOL) {
eol = true;
}
else {
tempZ = tokens.nval;
c = tokens.nextToken();
if (c == StreamTokenizer.TT_EOF) {
eof = true;
}
else if (c == StreamTokenizer.TT_EOL) {
eol = true;
}
else {
tempRad = tokens.nval;
c = tokens.nextToken();
if (c == StreamTokenizer.TT_EOF) {
eof = true;
}
else if (c == StreamTokenizer.TT_EOL) {
eol = true;
}
else {
tempLink = (int) tokens.nval;
++linesRead;
}
LineArray[linesRead] = new swcparts.SwcLine();
LineArray[linesRead].setLine(tempLineNum,
tempLineNum, tempLineType, tempX, tempY,
tempZ, tempRad, tempLink);
if (tempLink > 0) {
LineArray[tempLink].setNumDaughters(
LineArray[
tempLink].getNumDaughters() + 1);
LineArray[tempLineNum].setSegLength(
LineArray[
tempLineNum].getDistance(LineArray[
tempLink]));
if (LineArray[tempLink].getNumDaughters() ==
1) {
LineArray[tempLink].setDaughter1num(
tempLineNum);
}
else if (LineArray[tempLink].getNumDaughters() ==
2) {
LineArray[tempLink].setDaughter2num(
tempLineNum);
}
}
}
}
}
}
}
}
}
NeuronSize = linesRead;
this.setLengths();
this.setStemsStartRadAndBO();
this.setAllLineTreeNumber();
// System.out.println(linesRead);
}
catch (IOException e) {
System.out.println("error: " + e.getMessage());
System.out.println("Hit enter.");
char character;
try {
character = (char) System.in.read();
}
catch (IOException f) {}
}
}
//returns the cell partition asymetry
public double getCellAsymetry(int typeToDo) {
// if (!this.getTermDegreeSet()) {
// this.setTermDegrees();
// }
double j = 0;
double asymSum = 0;
double asymMean = 0;
int tree1tips = 0;
int tree2tips = 0;
// double tempAsyem = 0;
for (int i = 2; i < NeuronSize; ++i) {
if ( (this.getNumDaughters(i) == 2) && (this.getType(i) == typeToDo)) {
j += 1;
tree1tips = (this.getTreeSize(this.getDaughter1num(i)) + 1);
tree2tips = (this.getTreeSize(this.getDaughter2num(i)) + 1);
if ( (tree1tips == 1) && (tree2tips == 1)) {
asymSum += 0;
// tempAsyem = 0;
}
else {
asymSum += Math.abs(tree1tips - tree2tips) /
(tree1tips + tree2tips - 2);
// tempAsyem = Math.abs(tree1tips - tree2tips) /
// (tree1tips + tree2tips - 2);
}
}
}
asymMean = asymSum / j;
return asymMean;
}
//returns the partition asymetry from the curent point on
public double getTreeAsymetry(int currentBranch, int typeToDo) {
double j = 0;
double asymSum = 0;
double asymMean = 0;
double tree1tips = 0;
double tree2tips = 0;
int currentTreeNum = this.getTreeNumber(currentBranch);
// double tempAsyem = 0;
for (int i = currentBranch; i < NeuronSize; ++i) {
if ( (this.getNumDaughters(i) == 2) && (this.getType(i) == typeToDo)) {
if (this.getTreeNumber(i) == currentTreeNum) {
j += 1;
tree1tips = (this.getTreeSize(this.getDaughter1num(i)) + 1);
// System.out.println("tree 1 tips = "+tree1tips);
tree2tips = (this.getTreeSize(this.getDaughter2num(i)) + 1);
// System.out.println("tree 2 tips = "+tree2tips);
if ( (tree1tips == 1) && (tree2tips == 1)) {
asymSum += 0;
// tempAsyem = 0;
}
else {
asymSum += Math.abs(tree1tips - tree2tips) /
(tree1tips + tree2tips - 2);
// tempAsyem = ( (Math.abs(tree1tips - tree2tips)) /
// (tree1tips + tree2tips - 2));
}
// System.out.println("asymSum, j = "+asymSum+" "+j);
//System.out.println(" tree num, line number, Tips1, Tips2, Asyem : " +
// currentTreeNum + " " + i + " " + tree1tips + " " +
// tree2tips + " " + tempAsyem);
}
}
}
if (j == 0) {
return 0;
}
if (asymSum == 0) {
return 0;
}
asymMean = asymSum / j;
return asymMean;
}
//returns the surface area from the curent point on
public double getTreeSurface(int currentBranch, int typeToDo) {
double tempSurface = 0;
int k = currentBranch;
double currentLength = 0;
if (this.getType(k) != typeToDo) {
return 0;
}
while (this.getNumDaughters(k) == 1) {
currentLength = LineArray[k].getDistance(LineArray[this.getDaughter1num(k)]);
tempSurface += (Math.PI*(this.getRad(k)+this.getRad(this.getDaughter1num(k)))*currentLength);
//System.out.println(currentLength +" "+this.getRad(k)+" "+this.getRad(this.getDaughter1num(k))+" "+Math.PI*(this.getRad(k)+this.getRad(this.getDaughter1num(k)))*currentLength);
k = this.getDaughter1num(k);
}
if (this.getNumDaughters(k) == 0) {
return tempSurface;
}
if (this.getNumDaughters(k) == 2) {
if ( (this.getType(this.getDaughter1num(k)) != typeToDo) &&
(this.getType(this.getDaughter1num(k)) != typeToDo)) {
tempSurface += 0;
}
else if (this.getType(this.getDaughter1num(k)) != typeToDo) {
tempSurface += this.getTreeSurface(this.getDaughter2num(k), typeToDo);
}
else if (this.getType(this.getDaughter2num(k)) != typeToDo) {
tempSurface += this.getTreeSurface(this.getDaughter1num(k), typeToDo);
}
else {
tempSurface += this.getTreeSurface(this.getDaughter1num(k), typeToDo) +
this.getTreeSurface(this.getDaughter2num(k), typeToDo);
}
return tempSurface;
}
return 0;
}
//returns the surface area asymetry from the curent point on
public double getTreeSurfaceAsym(int currentBranch, int typeToDo) {
double j = 0;
double asymSum = 0;
double asymMean = 0;
double tree1surface = 0;
double tree2surface = 0;
int currentTreeNum = this.getTreeNumber(currentBranch);
// double tempAsyem = 0;
for (int i = currentBranch; i < NeuronSize; ++i) {
if ( (this.getNumDaughters(i) == 2) && (this.getType(i) == typeToDo)) {
if (this.getTreeNumber(i) == currentTreeNum) {
j += 1;
tree1surface = (this.getTreeSurface(this.getDaughter1num(i),typeToDo));
tree2surface = (this.getTreeSurface(this.getDaughter2num(i), typeToDo));
if ( (tree1surface == tree2surface)) {
asymSum += 0;
}
else {
asymSum += Math.abs(tree1surface - tree2surface) /
(tree1surface + tree2surface);
}
}
}
}
if (j == 0) {
return 0;
}
if (asymSum == 0) {
return 0;
}
asymMean = asymSum / j;
return asymMean;
}
//function to remove axons from basal trees (for example) so they don't mess up branch order/tree size calcs
public void removeOtherTypeSideBranches(int type) {
for (int i = 2; i < NeuronSize; ++i) {
if ( (LineArray[i].getLineType() == type) && (LineArray[i].getNumDaughters() == 2)) {
if ( (LineArray[LineArray[i].getDaughter1num()].getLineType() != type) &&
(LineArray[LineArray[i].getDaughter2num()].getLineType() != type)) {
LineArray[i].setNumDaughters(0);
LineArray[i].setDaughter1num(0);
LineArray[i].setDaughter2num(0);
}
else if (LineArray[LineArray[i].getDaughter1num()].getLineType() != type) {
LineArray[i].setNumDaughters(1);
LineArray[i].setDaughter1num(LineArray[i].getDaughter2num());
}
else if (LineArray[LineArray[i].getDaughter2num()].getLineType() != type) {
LineArray[i].setNumDaughters(1);
LineArray[i].setDaughter2num(0);
}
}
else if ( (LineArray[i].getLineType() == type) &&
(LineArray[i].getNumDaughters() == 1)) {
if (LineArray[LineArray[i].getDaughter1num()].getLineType() != type) {
LineArray[i].setNumDaughters(0);
LineArray[i].setDaughter1num(0);
}
}
}
}
public int getSize() {
return NeuronSize;
}
public double getSegLength(int linenum) {
return this.LineArray[linenum].getSegLength();
}
public int getBranchOrder(int linenum) {
return this.LineArray[linenum].getBranchOrder();
}
public double getBranchStartRad(int linenum) {
return this.LineArray[linenum].getBranchStartRad();
}
public int getStemNumber(int linenum) {
return this.LineArray[linenum].getStemNumber();
}
public int getTreeNumber(int linenum) {
return this.LineArray[linenum].getLineTreeNum();
}
public double getTreeLength(int linenum) {
return this.LineArray[linenum].getTreeLength();
}
public double getEDistanceToSoma(int linenum) {
return this.LineArray[linenum].getEuclidianDistance();
}
public double getBranchLength(int linenum) {
return this.LineArray[linenum].getBranchLength();
}
public double getZ(int linenum) {
return LineArray[linenum].getLineZ();
}
public double getY(int linenum) {
return LineArray[linenum].getLineY();
}
public double getX(int linenum) {
return LineArray[linenum].getLineX();
}
public double getRad(int linenum) {
return LineArray[linenum].getLineRad();
}
public int getLink(int linenum) {
return LineArray[linenum].getLineLink();
}
public int getType(int linenum) {
return LineArray[linenum].getLineType();
}
public int getNumDaughters(int linenum) {
return LineArray[linenum].getNumDaughters();
}
public int getDaughter1num(int linenum) {
return LineArray[linenum].getDaughter1num();
}
public int getDaughter2num(int linenum) {
return LineArray[linenum].getDaughter2num();
}
public double getDaughter1rad(int linenum) {
return LineArray[LineArray[linenum].getDaughter1num()].getLineRad();
}
public double getDaughter2rad(int linenum) {
return LineArray[LineArray[linenum].getDaughter2num()].getLineRad();
}
public double getParentRad(int linenum) {
return LineArray[LineArray[linenum].getLineLink()].getLineRad();
}
// check for type 1.1 and 1.2 errors
public void badType(PrintWriter fileout) {
for (int i = 2; i < NeuronSize; ++i) {
if ( (LineArray[i].getLineType() < (1)) || (LineArray[i].getLineType() > (10))) {
fileout.println("1.1 Line " + LineArray[i].getOrigionalLineNum() + " of type " +
LineArray[i].getLineType() +
" is of an invalid type. (no action taken)");
fileout.println(" B1 X, Y, Z: " + this.getX(i) + " " + this.getY(i) +
" " + this.getZ(i));
}
if ( (LineArray[i].getLineType() !=
LineArray[LineArray[i].getLineLink()].getLineType()) &&
(LineArray[LineArray[i].getLineLink()].getLineType() != 1)) {
fileout.println("1.2 Line " + LineArray[i].getOrigionalLineNum() + " of type " +
LineArray[i].getLineType() + " links to incorrect type " +
LineArray[LineArray[i].getLineLink()].getLineType() +
". (no action taken)");
fileout.println(" B1 X, Y, Z: " + this.getX(i) + " " + this.getY(i) +
" " + this.getZ(i));
}
}
}
// check for type 1.1 and 1.2 errors
public void badTypeFix(PrintWriter fileout) {
for (int i = 2; i < NeuronSize; ++i) {
if ( (LineArray[i].getLineType() < (1)) || (LineArray[i].getLineType() > (10))) {
fileout.print("1.1 Line " + LineArray[i].getOrigionalLineNum() + " of type " +
LineArray[i].getLineType() + " is of an invalid type");
if ( (LineArray[i].getLineType() !=
LineArray[LineArray[i].getLineLink()].getLineType()) &&
(LineArray[LineArray[i].getLineLink()].getLineType() ==
this.getFirstDaughterNumType(i))) {
LineArray[i].setLineType(LineArray[LineArray[i].getLineLink()].getLineType());
fileout.println(" (type changed to " +
LineArray[LineArray[i].getLineLink()].getLineType() + ")");
fileout.println(" A X, Y, Z: " + this.getX(i) + " " +
this.getY(i) + " " + this.getZ(i));
}
else {
fileout.println(". (no action taken)");
fileout.println(" B1 X, Y, Z: " + this.getX(i) + " " +
this.getY(i) + " " + this.getZ(i));
}
}
if ( (LineArray[i].getLineType() !=
LineArray[LineArray[i].getLineLink()].getLineType()) &&
(LineArray[LineArray[i].getLineLink()].getLineType() != 1)) {
fileout.print("1.2 Line " + LineArray[i].getOrigionalLineNum() + " of type " +
LineArray[i].getLineType() + " links to incorrect type " +
LineArray[LineArray[i].getLineLink()].getLineType());
if (LineArray[LineArray[i].getLineLink()].getLineType() ==
this.getFirstDaughterNumType(i)) {
LineArray[i].setLineType(LineArray[LineArray[i].getLineLink()].getLineType());
fileout.println(" (type changed to " +
LineArray[LineArray[i].getLineLink()].getLineType() + ")");
fileout.println(" A X, Y, Z: " + this.getX(i) + " " +
this.getY(i) + " " + this.getZ(i));
}
else {
fileout.println(". (no action taken)");
fileout.println(" B1 X, Y, Z: " + this.getX(i) + " " +
this.getY(i) + " " + this.getZ(i));
}
}
}
}
//check for type 2.2
public void badStartType(PrintWriter fileout) {
if (LineArray[1].getLineType() != 1) {
fileout.println("2.2 Line 1 is of type " + LineArray[1].getLineType() +
". (no action taken)");
fileout.println(" B1 X, Y, Z: " + this.getX(1) + this.getY(1) +
this.getZ(1));
}
}
//check for type 3.4
public void badStartLink(PrintWriter fileout) {
if (LineArray[1].getLineLink() != -1) {
fileout.println("2.2 Line 1 links to " + LineArray[1].getLineLink() +
". (no action taken)");
fileout.println(" B1 X, Y, Z: " + this.getX(1) + this.getY(1) +
this.getZ(1));
}
}
//check for and fix type 3.4
public void badStartLinkFix(PrintWriter fileout) {
if (LineArray[1].getLineLink() != -1) {
fileout.println("2.2 Line 1 links to " + LineArray[1].getLineLink() +
" Changed to -1");
fileout.println(" A X, Y, Z: " + this.getX(1) + this.getY(1) +
this.getZ(1));
LineArray[1].setLineLink( -1);
}
}
//check for type 3.3
public void pointSoma(PrintWriter fileout) {
if (this.countType(1) == 1) {
fileout.println("3.3 Line 1 links to " + LineArray[1].getLineLink() +
". (no action taken)");
fileout.println(" B1 X, Y, Z: " + this.getX(1) + this.getY(1) +
this.getZ(1));
}
}
//check for type 4.1, 4.2, 4.3) min value, max stdev,
public void badRadMinStdev(PrintWriter fileout, double min, double stDevm) {
double mean = this.meanRad();
double stdev = this.stdevRad();
for (int i = 2; i < NeuronSize; ++i) {
if (LineArray[i].getLineRad() == 0) {
fileout.println("4.1 Radius of line " + LineArray[i].getOrigionalLineNum() +
" = 0. (no action taken) ");
fileout.println(" B1 X, Y, Z: " + this.getX(i) + " " + this.getY(i) +
" " + this.getZ(i));
}
else if (LineArray[i].getLineRad() <= min) {
fileout.println("4.2 Radius of line " + LineArray[i].getOrigionalLineNum() +
" at " + LineArray[i].getLineRad() + " <= " + min +
". (no action taken)");
fileout.println(" B1 X, Y, Z: " + this.getX(i) + " " + this.getY(i) +
" " + this.getZ(i));
}
if (LineArray[i].getLineRad() - mean >= stdev * stDevm) {
fileout.println("4.3 Radius of line " + LineArray[i].getOrigionalLineNum() +
" at " + LineArray[i].getLineRad() + " >= " + stDevm +
" stdevs. (no action taken)");
fileout.println(" B1 X, Y, Z: " + this.getX(i) + " " + this.getY(i) +
" " + this.getZ(i));
}
}
}
//check for type 4.1, 4.2, 4.3)terminal stdev, min value, max stdev,
public void badRadTermsMinStdev(PrintWriter fileout, double terms, double min, double stDevm) {
double termMax = (this.meanTermRad() + (terms * this.stdevTermRad()));
double mean = this.meanRad();
double stdev = this.stdevRad();
for (int i = 2; i < NeuronSize; ++i) {
if (LineArray[i].getLineRad() == 0) {
fileout.println("4.1 Radius of line " + LineArray[i].getOrigionalLineNum() +
" = 0. (no action taken) ");
fileout.println(" B1 X, Y, Z: " + this.getX(i) + " " + this.getY(i) +
" " + this.getZ(i));
}
else if (LineArray[i].getLineRad() <= min) {
fileout.println("4.2 Radius of line " + LineArray[i].getOrigionalLineNum() +
" at " + LineArray[i].getLineRad() + " <= " + min +
". (no action taken)");
fileout.println(" B1 X, Y, Z: " + this.getX(i) + " " + this.getY(i) +
" " + this.getZ(i));
}
if ( (LineArray[i].getLineRad() >= termMax) && (LineArray[i].getNumDaughters() == 0)) {
fileout.println("4.5 Radius of terminal line " +
LineArray[i].getOrigionalLineNum() + " at " +
LineArray[i].getLineRad() + " >= " + terms +
" stdevs above the mean. (no action taken)");
fileout.println(" B1 X, Y, Z: " + this.getX(i) + " " + this.getY(i) +
" " + this.getZ(i));
}
else if (LineArray[i].getLineRad() - mean >= stdev * stDevm) {
fileout.println("4.3 Radius of line " + LineArray[i].getOrigionalLineNum() +
" at " + LineArray[i].getLineRad() + " >= " + stDevm +
" stdevs above the mean. (no action taken)");
fileout.println(" B1 X, Y, Z: " + this.getX(i) + " " + this.getY(i) +
" " + this.getZ(i));
}
}
}
//check for type 4.1, 4.2, 4.3)min value, max stdev, type
public void badRadMinStdev(PrintWriter fileout, double min, double stDevm, int type) {
double mean = this.meanRad(type);
double stdev = this.stdevRad(type);
for (int i = 2; i < NeuronSize; ++i) {
if ( (LineArray[i].getLineType() == type)) {
if (LineArray[i].getLineRad() == 0) {
fileout.println("4.1 Radius of line " + LineArray[i].getOrigionalLineNum() +
" = 0. (no action taken) ");
fileout.println(" B1 X, Y, Z: " + this.getX(i) + " " +
this.getY(i) + " " + this.getZ(i));
}
else if (LineArray[i].getLineRad() <= min) {
fileout.println("4.2 Radius of line " + LineArray[i].getOrigionalLineNum() +
" at " + LineArray[i].getLineRad() + " <= " + min +
". (no action taken)");
fileout.println(" B1 X, Y, Z: " + this.getX(i) + " " +
this.getY(i) + " " + this.getZ(i));
}
if (LineArray[i].getLineRad() - mean >= stdev * stDevm) {
fileout.println("4.3 Radius of line " + LineArray[i].getOrigionalLineNum() +
" at " + LineArray[i].getLineRad() + " >= " + stDevm +
" stdevs above the mean. (no action taken)");
fileout.println(" B1 X, Y, Z: " + this.getX(i) + " " +
this.getY(i) + " " + this.getZ(i));
}
}
}
}
//check for type 4.1, 4.2, 4.3) terminal stdev, min value, max stdev, type
public void badRadTermsMinStdev(PrintWriter fileout, double terms, double min, double stDevm,
int type) {
double termMax = (this.meanTermRad(type) + (terms * this.stdevTermRad(type)));
double mean = this.meanRad(type);
double stdev = this.stdevRad(type);
for (int i = 2; i < NeuronSize; ++i) {
if ( (LineArray[i].getLineType() == type)) {
if (LineArray[i].getLineRad() == 0) {
fileout.println("4.1 Radius of line " + LineArray[i].getOrigionalLineNum() +
" = 0. (no action taken) ");
fileout.println(" B1 X, Y, Z: " + this.getX(i) + " " +
this.getY(i) + " " + this.getZ(i));
}
else if (LineArray[i].getLineRad() <= min) {
fileout.println("4.2 Radius of line " + LineArray[i].getOrigionalLineNum() +
" at " + LineArray[i].getLineRad() + " <= " + min +
". (no action taken)");
fileout.println(" B1 X, Y, Z: " + this.getX(i) + " " +
this.getY(i) + " " + this.getZ(i));
}
if ( (LineArray[i].getLineRad() >= termMax) &&
(LineArray[i].getNumDaughters() == 0)) {
fileout.println("4.5 Radius of terminal line " +
LineArray[i].getOrigionalLineNum() + " at " +
LineArray[i].getLineRad() + " >= " + terms +
" stdevs above the mean. (no action taken)");
fileout.println(" B1 X, Y, Z: " + this.getX(i) + " " +
this.getY(i) + " " + this.getZ(i));
}
else if (LineArray[i].getLineRad() - mean >= stdev * stDevm) {
fileout.println("4.3 Radius of line " + LineArray[i].getOrigionalLineNum() +
" at " + LineArray[i].getLineRad() + " >= " + stDevm +
" stdevs above the mean. (no action taken)");
fileout.println(" B1 X, Y, Z: " + this.getX(i) + " " +
this.getY(i) + " " + this.getZ(i));
}
}
}
}
//check for type 4.1, 4.2, 4.3) terminal stdev, min value, max stdev, type
public void badRadTermsMinStdevFixZero(PrintWriter fileout, double terms, double min,
double stDevm, int type) {
double termMax = (this.meanTermRad(type) + (terms * this.stdevTermRad(type)));
double mean = this.meanRad(type);
double stdev = this.stdevRad(type);
for (int i = 2; i <= NeuronSize; ++i) {
if ( (LineArray[i].getLineType() == type)) {
if (LineArray[i].getLineRad() == 0) {
LineArray[i].setLineRadius(LineArray[LineArray[i].getLineLink()].
getLineRad());
fileout.println("4.1 Radius of line " + LineArray[i].getOrigionalLineNum() +
" = 0. (changed to " +
LineArray[LineArray[i].getLineLink()].getLineRad() +
")");
fileout.println(" A X, Y, Z: " + this.getX(i) + " " +
this.getY(i) + " " + this.getZ(i));
}
else if (LineArray[i].getLineRad() <= min) {
fileout.println("4.2 Radius of line " + LineArray[i].getOrigionalLineNum() +
" at " + LineArray[i].getLineRad() + " <= " + min +
". (no action taken)");
fileout.println(" B1 X, Y, Z: " + this.getX(i) + " " +
this.getY(i) + " " + this.getZ(i));
}
if ( (LineArray[i].getLineRad() >= termMax) &&
(LineArray[i].getNumDaughters() == 0)) {
fileout.println("4.5 Radius of terminal line " +
LineArray[i].getOrigionalLineNum() + " at " +
LineArray[i].getLineRad() + " >= " + terms +
" stdevs above the mean. (no action taken)");
fileout.println(" B1 X, Y, Z: " + this.getX(i) + " " +
this.getY(i) + " " + this.getZ(i));
}
else if (LineArray[i].getLineRad() - mean >= stdev * stDevm) {
fileout.println("4.3 Radius of line " + LineArray[i].getOrigionalLineNum() +
" at " + LineArray[i].getLineRad() + " >= " + stDevm +
" stdevs above the mean. (no action taken)");
fileout.println(" B1 X, Y, Z: " + this.getX(i) + " " +
this.getY(i) + " " + this.getZ(i));
}
}
}
}
//check for type 4.1, 4.2, 4.3)
public void badRadTermsMinMax(PrintWriter fileout, double terms, double min, double max) {
double termMax = (this.meanTermRad() + (terms * this.stdevTermRad()));
for (int i = 2; i < NeuronSize; ++i) {
if (LineArray[i].getLineRad() == 0) {
fileout.println("4.1 Radius of line " + LineArray[i].getOrigionalLineNum() +
" = 0 . (no action taken)");
fileout.println(" B1 X, Y, Z: " + this.getX(i) + " " + this.getY(i) +
" " + this.getZ(i));
}
else if (LineArray[i].getLineRad() <= min) {
fileout.println("4.2 Radius of line " + LineArray[i].getOrigionalLineNum() +
" at " + LineArray[i].getLineRad() + " <= " + min +
". (no action taken)");
fileout.println(" B1 X, Y, Z: " + this.getX(i) + " " + this.getY(i) +
" " + this.getZ(i));
}
if ( (LineArray[i].getLineRad() >= termMax) && (LineArray[i].getNumDaughters() == 0)) {
fileout.println("4.5 Radius of terminal line " +
LineArray[i].getOrigionalLineNum() + " at " +
LineArray[i].getLineRad() + " >= " + terms +
" stdevs above the mean. (no action taken)");
fileout.println(" B1 X, Y, Z: " + this.getX(i) + " " + this.getY(i) +
" " + this.getZ(i));
}
else if (LineArray[i].getLineRad() >= max) {
fileout.println("4.3 Radius of line " + LineArray[i].getOrigionalLineNum() +
" at " + LineArray[i].getLineRad() + " >= " + max +
". (no action taken)");
fileout.println(" B1 X, Y, Z: " + this.getX(i) + " " + this.getY(i) +
" " + this.getZ(i));
}
}
}
//check for type 4.1, 4.2, 4.3)
public void badRadMinMax(PrintWriter fileout, double min, double max) {
for (int i = 2; i < NeuronSize; ++i) {
if (LineArray[i].getLineRad() == 0) {
fileout.println("4.1 Radius of line " + LineArray[i].getOrigionalLineNum() +
" = 0 . (no action taken)");
fileout.println(" B1 X, Y, Z: " + this.getX(i) + " " + this.getY(i) +
" " + this.getZ(i));
}
else if (LineArray[i].getLineRad() <= min) {
fileout.println("4.2 Radius of line " + LineArray[i].getOrigionalLineNum() +
" at " + LineArray[i].getLineRad() + " <= " + min +
". (no action taken)");
fileout.println(" B1 X, Y, Z: " + this.getX(i) + " " + this.getY(i) +
" " + this.getZ(i));
}
if (LineArray[i].getLineRad() >= max) {
fileout.println("4.3 Radius of line " + LineArray[i].getOrigionalLineNum() +
" at " + LineArray[i].getLineRad() + " >= " + max +
". (no action taken)");
fileout.println(" B1 X, Y, Z: " + this.getX(i) + " " + this.getY(i) +
" " + this.getZ(i));
}
}
}
//check for type 4.1, 4.2, 4.3)
public void badRadMinMax(PrintWriter fileout, double min, double max, int type) {
for (int i = 2; i < NeuronSize; ++i) {
if ( (LineArray[i].getLineType() == type)) {
if (LineArray[i].getLineRad() == 0) {
fileout.println("4.1 Radius of line " + LineArray[i].getOrigionalLineNum() +
" = 0. (no action taken) ");
fileout.println(" B1 X, Y, Z: " + this.getX(i) + " " +
this.getY(i) + " " + this.getZ(i));
}
else if (LineArray[i].getLineRad() <= min) {
fileout.println("4.2 Radius of line " + LineArray[i].getOrigionalLineNum() +
" at " + LineArray[i].getLineRad() + " <= " + min +
". (no action taken)");
fileout.println(" B1 X, Y, Z: " + this.getX(i) + " " +
this.getY(i) + " " + this.getZ(i));
}
if (LineArray[i].getLineRad() >= max) {
fileout.println("4.3 Radius of line " + LineArray[i].getOrigionalLineNum() +
" at " + LineArray[i].getLineRad() + " >= " + max +
". (no action taken)");
fileout.println(" B1 X, Y, Z: " + this.getX(i) + " " +
this.getY(i) + " " + this.getZ(i));
}
}
}
}
//check for type 4.1, 4.2, 4.3)
public void badRadTermsMinMax(PrintWriter fileout, double terms, double min, double max,
int type) {
double termMax = (this.meanTermRad(type) + (terms * this.stdevTermRad(type)));
for (int i = 2; i < NeuronSize; ++i) {
if ( (LineArray[i].getLineType() == type)) {
if (LineArray[i].getLineRad() == 0) {
fileout.println("4.1 Radius of line " + LineArray[i].getOrigionalLineNum() +
" = 0. (no action taken) ");
fileout.println(" B1 X, Y, Z: " + this.getX(i) + " " +
this.getY(i) + " " + this.getZ(i));
}
else if (LineArray[i].getLineRad() <= min) {
fileout.println("4.2 Radius of line " + LineArray[i].getOrigionalLineNum() +
" at " + LineArray[i].getLineRad() + " <= " + min +
". (no action taken)");
fileout.println(" B1 X, Y, Z: " + this.getX(i) + " " +
this.getY(i) + " " + this.getZ(i));
}
if ( (LineArray[i].getLineRad() >= termMax) &&
(LineArray[i].getNumDaughters() == 0)) {
fileout.println("4.5 Radius of terminal line " +
LineArray[i].getOrigionalLineNum() + " at " +
LineArray[i].getLineRad() + " >= " + terms +
" stdevs above the mean. (no action taken)");
fileout.println(" B1 X, Y, Z: " + this.getX(i) + " " +
this.getY(i) + " " + this.getZ(i));
}
else if (LineArray[i].getLineRad() >= max) {
fileout.println("4.3 Radius of line " + LineArray[i].getOrigionalLineNum() +
" at " + LineArray[i].getLineRad() + " >= " + max +
". (no action taken)");
fileout.println(" B1 X, Y, Z: " + this.getX(i) + " " +
this.getY(i) + " " + this.getZ(i));
}
}
}
}
//check for type 4.1, 4.2, 4.3)
public void badRadMinMaxFixZero(PrintWriter fileout, double min, double max) {
for (int i = 2; i < NeuronSize; ++i) {
if (LineArray[i].getLineRad() == 0) {
LineArray[i].setLineRadius(LineArray[LineArray[i].getLineLink()].getLineRad());
fileout.println("4.1 Radius of line " + LineArray[i].getOrigionalLineNum() +
" = 0. (changed to " +
LineArray[LineArray[i].getLineLink()].getLineRad() + ")");
fileout.println(" B1 X, Y, Z: " + this.getX(i) + " " + this.getY(i) +
" " + this.getZ(i));
}
else if (LineArray[i].getLineRad() <= min) {
fileout.println("4.2 Radius of line " + LineArray[i].getOrigionalLineNum() +
" at " + LineArray[i].getLineRad() + " <= " + min +
". (no action taken)");
fileout.println(" B1 X, Y, Z: " + this.getX(i) + " " + this.getY(i) +
" " + this.getZ(i));
}
if (LineArray[i].getLineRad() >= max) {
fileout.println("4.3 Radius of line " + LineArray[i].getOrigionalLineNum() +
" at " + LineArray[i].getLineRad() + " >= " + max +
". (no action taken)");
fileout.println(" B1 X, Y, Z: " + this.getX(i) + " " + this.getY(i) +
" " + this.getZ(i));
}
}
}
//check for type 4.1, 4.2, 4.3)
public void badRadTermsMinMaxFixZero(PrintWriter fileout, double terms, double min, double max) {
double termMax = (this.meanTermRad() + (terms * this.stdevTermRad()));
for (int i = 2; i < NeuronSize; ++i) {
if (LineArray[i].getLineRad() == 0) {
LineArray[i].setLineRadius(LineArray[LineArray[i].getLineLink()].getLineRad());
fileout.println("4.1 Radius of line " + LineArray[i].getOrigionalLineNum() +
" = 0. (changed to " +
LineArray[LineArray[i].getLineLink()].getLineRad() + ")");
fileout.println(" B1 X, Y, Z: " + this.getX(i) + " " + this.getY(i) +
" " + this.getZ(i));
}
else if (LineArray[i].getLineRad() <= min) {
fileout.println("4.2 Radius of line " + LineArray[i].getOrigionalLineNum() +
" at " + LineArray[i].getLineRad() + " <= " + min +
". (no action taken)");
fileout.println(" B1 X, Y, Z: " + this.getX(i) + " " + this.getY(i) +
" " + this.getZ(i));
}
if ( (LineArray[i].getLineRad() >= termMax) && (LineArray[i].getNumDaughters() == 0)) {
fileout.println("4.5 Radius of terminal line " +
LineArray[i].getOrigionalLineNum() + " at " +
LineArray[i].getLineRad() + " >= " + terms +
" stdevs above the mean. (no action taken)");
fileout.println(" B1 X, Y, Z: " + this.getX(i) + " " + this.getY(i) +
" " + this.getZ(i));
}
else if (LineArray[i].getLineRad() >= max) {
fileout.println("4.3 Radius of line " + LineArray[i].getOrigionalLineNum() +
" at " + LineArray[i].getLineRad() + " >= " + max +
". (no action taken)");
fileout.println(" B1 X, Y, Z: " + this.getX(i) + " " + this.getY(i) +
" " + this.getZ(i));
}
}
}
//check for type 4.1, 4.2, 4.3)
public void badRadMinMaxFixZero(PrintWriter fileout, double min, double max, int type) {
for (int i = 2; i < NeuronSize; ++i) {
if ( (LineArray[i].getLineType() == type)) {
if (LineArray[i].getLineRad() == 0) {
LineArray[i].setLineRadius(LineArray[LineArray[i].getLineLink()].
getLineRad());
fileout.println("4.1 Radius of line " + LineArray[i].getOrigionalLineNum() +
" = 0. (changed to " +
LineArray[LineArray[i].getLineLink()].getLineRad() +
")");
fileout.println(" A X, Y, Z: " + this.getX(i) + " " +
this.getY(i) + " " + this.getZ(i));
}
else if (LineArray[i].getLineRad() <= min) {
fileout.println("4.2 Radius of line " + LineArray[i].getOrigionalLineNum() +
" at " + LineArray[i].getLineRad() + " <= " + min +
". (no action taken)");
fileout.println(" B1 X, Y, Z: " + this.getX(i) + " " +
this.getY(i) + " " + this.getZ(i));
}
if (LineArray[i].getLineRad() >= max) {
fileout.println("4.3 Radius of line " + LineArray[i].getOrigionalLineNum() +
" at " + LineArray[i].getLineRad() + " >= " + max +
". (no action taken)");
fileout.println(" B1 X, Y, Z: " + this.getX(i) + " " +
this.getY(i) + " " + this.getZ(i));
}
}
}
}
//check for type 4.1, 4.2, 4.3)
public void badRadTermsMinMaxFixZero(PrintWriter fileout, double terms, double min, double max,
int type) {
double termMax = (this.meanTermRad(type) + (terms * this.stdevTermRad(type)));
for (int i = 2; i < NeuronSize; ++i) {
if ( (LineArray[i].getLineType() == type)) {
if (LineArray[i].getLineRad() == 0) {
LineArray[i].setLineRadius(LineArray[LineArray[i].getLineLink()].
getLineRad());
fileout.println("4.1 Radius of line " + LineArray[i].getOrigionalLineNum() +
" = 0. (changed to " +
LineArray[LineArray[i].getLineLink()].getLineRad() +
")");
fileout.println(" A X, Y, Z: " + this.getX(i) + " " +
this.getY(i) + " " + this.getZ(i));
}
else if (LineArray[i].getLineRad() <= min) {
fileout.println("4.2 Radius of line " + LineArray[i].getOrigionalLineNum() +
" at " + LineArray[i].getLineRad() + " <= " + min +
". (no action taken)");
fileout.println(" B1 X, Y, Z: " + this.getX(i) + " " +
this.getY(i) + " " + this.getZ(i));
}
if ( (LineArray[i].getLineRad() >= termMax) &&
(LineArray[i].getNumDaughters() == 0)) {
fileout.println("4.5 Radius of terminal line " +
LineArray[i].getOrigionalLineNum() + " at " +
LineArray[i].getLineRad() + " >= " + terms +
" stdevs above the mean. (no action taken)");
fileout.println(" B1 X, Y, Z: " + this.getX(i) + " " +
this.getY(i) + " " + this.getZ(i));
}
else if (LineArray[i].getLineRad() >= max) {
fileout.println("4.3 Radius of line " + LineArray[i].getOrigionalLineNum() +
" at " + LineArray[i].getLineRad() + " >= " + max +
". (no action taken)");
fileout.println(" B1 X, Y, Z: " + this.getX(i) + " " +
this.getY(i) + " " + this.getZ(i));
}
}
}
}
//compute mean radius in a neuron
public double meanRad() {
double mean = 0.0;
if (this.NeuronSize > 0) {
double sum = 0.0;
for (int i = 1; i < this.NeuronSize; i++) {
sum += this.LineArray[i].getLineRad();
}
mean = sum / (this.NeuronSize);
}
return mean;
}
//comput mean radius for given type in a neuron
public double meanRad(int type) {
double mean = 0.0;
if (this.NeuronSize > 0) {
double sum = 0.0;
int count = 0;
for (int i = 1; i < this.NeuronSize; i++) {
if (this.LineArray[i].getLineType() == type) {
sum += this.LineArray[i].getLineRad();
++count;
}
}
mean = sum / count;
}
return mean;
}
//comput mean radius for terminal points for given type in a neuron f
public double meanTermRad(int type) {
double mean = 0.0;
if (this.NeuronSize > 0) {
double sum = 0.0;
int count = 0;
for (int i = 1; i < this.NeuronSize; i++) {
if ( (this.LineArray[i].getLineType() == type) &&
(this.LineArray[i].getNumDaughters() == 0)) {
sum += this.LineArray[i].getLineRad();
++count;
}
}
mean = sum / count;
}
return mean;
}
//comput mean radius for terminal points in a neuron
public double meanTermRad() {
double mean = 0.0;
if (this.NeuronSize > 0) {
double sum = 0.0;
int count = 0;
for (int i = 1; i < this.NeuronSize; i++) {
if (this.LineArray[i].getNumDaughters() == 0) {
sum += this.LineArray[i].getLineRad();
++count;
}
}
mean = sum / count;
}
return mean;
}
//compute standard deviaion of radii in a cell
public double stdevRad() {
double stdDev = 0.0;
if (this.NeuronSize > 1) {
double meanValue = this.meanRad();
double sum = 0.0;
for (int i = 1; i < this.NeuronSize; i++) {
double diff = LineArray[i].getLineRad() - meanValue;
sum += diff * diff;
}
stdDev = Math.sqrt(sum / (this.NeuronSize - 1));
}
return stdDev;
}
//compute standard deviaion of radii for a given type in a cell
public double stdevRad(int type) {
double stdDev = 0.0;
int count = 0;
if (this.NeuronSize > 1) {
double meanValue = this.meanRad(type);
double sum = 0.0;
for (int i = 1; i < this.NeuronSize; i++) {
if (this.LineArray[i].getLineType() == type) {
double diff = LineArray[i].getLineRad() - meanValue;
sum += diff * diff;
++count;
}
}
stdDev = Math.sqrt(sum / (count - 1));
}
return stdDev;
}
//compute standard deviaion of radii for terminal points in a cell
public double stdevTermRad() {
double stdDev = 0.0;
int count = 0;
if (this.NeuronSize > 1) {
double meanValue = this.meanTermRad();
double sum = 0.0;
for (int i = 1; i < this.NeuronSize; i++) {
if (this.LineArray[i].getNumDaughters() == 0) {
double diff = LineArray[i].getLineRad() - meanValue;
sum += diff * diff;
++count;
}
}
stdDev = Math.sqrt(sum / (count - 1));
}
return stdDev;
}
//compute standard deviaion of radii for terminal points for a given type in a cell
public double stdevTermRad(int type) {
double stdDev = 0.0;
int count = 0;
if (this.NeuronSize > 1) {
double meanValue = this.meanTermRad(type);
double sum = 0.0;
for (int i = 1; i < this.NeuronSize; i++) {
if ( (this.LineArray[i].getLineType() == type) &&
(this.LineArray[i].getNumDaughters() == 0)) {
double diff = LineArray[i].getLineRad() - meanValue;
sum += diff * diff;
++count;
}
}
stdDev = Math.sqrt(sum / (count - 1));
}
return stdDev;
}
//*********************************************bad seg length functions*************************************
public void badSegLengthMinStdev(PrintWriter fileout, double min, double stDevm) {
double mean = this.meanSegLength();
double stdev = this.stdevSegLength();
for (int i = 2; i < NeuronSize; ++i) {
if (this.LineArray[i].getDistance(LineArray[LineArray[i].getLineLink()]) <= min) {
fileout.println("2.5 Length of segment ending at point " +
LineArray[i].getOrigionalLineNum() + " <= " + min + " at " +
myFormatter.format(this.LineArray[i].getDistance(LineArray[
LineArray[i].getLineLink()])) + ". (no action taken)");
fileout.println(" B1 X, Y, Z: " + this.getX(i) + " " + this.getY(i) +
" " + this.getZ(i));
}
if (this.LineArray[i].getDistance(LineArray[LineArray[i].getLineLink()]) - mean >=
stdev * stDevm) {
fileout.println("2.4 Length of segment ending at point " +
LineArray[i].getOrigionalLineNum() + " >= " + stDevm +
" stdevs above mean at " +
myFormatter.format(this.LineArray[i].getDistance(LineArray[
LineArray[i].getLineLink()])) + ". (no action taken)");
fileout.println(" B1 X, Y, Z: " + this.getX(i) + " " + this.getY(i) +
" " + this.getZ(i));
}
}
}
public void badSegLengthMinStdev(PrintWriter fileout, double min, double stDevm, int type) {
double mean = this.meanSegLength(type);
double stdev = this.stdevSegLength(type);
for (int i = 2; i < NeuronSize; ++i) {
if ( (LineArray[i].getLineType() == type)) {
if (this.LineArray[i].getDistance(LineArray[LineArray[i].getLineLink()]) <= min) {
fileout.println("2.5 Length of segment ending at point " +
LineArray[i].getOrigionalLineNum() + " <= " + min + " at " +
myFormatter.format(this.LineArray[i].getDistance(LineArray[
LineArray[i].getLineLink()])) + ". (no action taken)");
fileout.println(" B1 X, Y, Z: " + this.getX(i) + " " +
this.getY(i) + " " + this.getZ(i));
}
if (this.LineArray[i].getDistance(LineArray[LineArray[i].getLineLink()]) - mean >=
stdev * stDevm) {
fileout.println("2.4 Length of segment ending at point " +
LineArray[i].getOrigionalLineNum() + " >= " + stDevm +
" stdevs above mean at " +
myFormatter.format(this.LineArray[i].getDistance(LineArray[
LineArray[i].getLineLink()])) + ". (no action taken)");
fileout.println(" B1 X, Y, Z: " + this.getX(i) + " " +
this.getY(i) + " " + this.getZ(i));
}
}
}
}
public void badSegLengthMinMax(PrintWriter fileout, double min, double max) {
for (int i = 2; i < NeuronSize; ++i) {
if (this.LineArray[i].getDistance(LineArray[LineArray[i].getLineLink()]) <= min) {
fileout.println("2.5 Length of segment ending at point " +
LineArray[i].getOrigionalLineNum() + " <= " + min + " at " +
myFormatter.format(this.LineArray[i].getDistance(LineArray[
LineArray[i].getLineLink()])) + ". (no action taken)");
fileout.println(" B1 X, Y, Z: " + this.getX(i) + " " + this.getY(i) +
" " + this.getZ(i));
}
if (this.LineArray[i].getDistance(LineArray[LineArray[i].getLineLink()]) >= max) {
fileout.println("2.4 Length of segment ending at point " +
LineArray[i].getOrigionalLineNum() + " >= " + max + " at " +
myFormatter.format(this.LineArray[i].getDistance(LineArray[
LineArray[i].getLineLink()])) + ". (no action taken)");
fileout.println(" B1 X, Y, Z: " + this.getX(i) + " " + this.getY(i) +
" " + this.getZ(i));
}
}
}
public void badSegLengthMinMax(PrintWriter fileout, double min, double max, int type) {
for (int i = 2; i < NeuronSize; ++i) {
if ( (LineArray[i].getLineType() == type)) {
if (this.LineArray[i].getDistance(LineArray[LineArray[i].getLineLink()]) <= min) {
fileout.println("2.5 Length of segment ending at point " +
LineArray[i].getOrigionalLineNum() + " <= " + min + " at " +
myFormatter.format(this.LineArray[i].getDistance(LineArray[
LineArray[i].getLineLink()])) + ". (no action taken)");
fileout.println(" B1 X, Y, Z: " + this.getX(i) + " " +
this.getY(i) + " " + this.getZ(i));
}
if (this.LineArray[i].getDistance(LineArray[LineArray[i].getLineLink()]) >= max) {
fileout.println("2.4 Length of segment ending at point " +
LineArray[i].getOrigionalLineNum() + " >= " + max + " at " +
myFormatter.format(this.LineArray[i].getDistance(LineArray[
LineArray[i].getLineLink()])) + ". (no action taken)");
fileout.println(" B1 X, Y, Z: " + this.getX(i) + " " +
this.getY(i) + " " + this.getZ(i));
}
}
}
}
//*********************************************bad seg length functions with small lines removed*************************************
public void badSegLengthMinFixStdev(PrintWriter fileout, double min, double stDevm) {
double mean = this.meanSegLength();
double stdev = this.stdevSegLength();
boolean firstTimeThrough = true;
boolean linesRemoved = true;
while (linesRemoved) {
linesRemoved = false;
for (int i = 2; i < NeuronSize; ++i) {
if (this.LineArray[i].getDistance(LineArray[LineArray[i].getLineLink()]) <=
min) {
fileout.println("2.5 Seg length ending at pt. " +
this.LineArray[i].getOrigionalLineNum() + " <= " + min +
" at " +
myFormatter.format(this.LineArray[i].getDistance(
LineArray[
LineArray[i].getLineLink()])) + ". (removed");
fileout.println(" A X, Y, Z: " + this.getX(i) + " " +
this.getY(i) + " " + this.getZ(i));
this.removeLine(i);
linesRemoved = true;
}
if (firstTimeThrough) {
if (this.LineArray[i].getDistance(LineArray[LineArray[i].getLineLink()]) -
mean >= stdev * stDevm) {
fileout.println("2.4 Seg length ending at pt. " +
LineArray[i].getOrigionalLineNum() + " >= " + stDevm +
" stdevs above mean at " +
myFormatter.format(this.LineArray[i].getDistance(
LineArray[LineArray[i].getLineLink()])) +
". (no action taken)");
fileout.println(" B1 X, Y, Z: " + this.getX(i) + " " +
this.getY(i) + " " + this.getZ(i));
}
}
}
firstTimeThrough = false;
}
}
public void badSegLengthMinFixStdev(PrintWriter fileout, double min, double stDevm, int type) {
double mean = this.meanSegLength(type);
double stdev = this.stdevSegLength(type);
boolean firstTimeThrough = true;
boolean linesRemoved = true;
while (linesRemoved) {
linesRemoved = false;
for (int i = 2; i < NeuronSize; ++i) {
if ( (LineArray[i].getLineType() == type)) {
if (this.LineArray[i].getDistance(LineArray[LineArray[i].getLineLink()]) <=
min) {
fileout.println("2.5 Seg length ending at pt. " +
this.LineArray[i].getOrigionalLineNum() + " <= " +
min +
" at " +
myFormatter.format(this.LineArray[i].
getDistance(LineArray[LineArray[i].getLineLink()])) +
". (removed)");
fileout.println(" A X, Y, Z: " + this.getX(i) + " " +
this.getY(i) + " " + this.getZ(i));
this.removeLine(i);
linesRemoved = true;
}
if (firstTimeThrough) {
if (this.LineArray[i].getDistance(LineArray[LineArray[i].getLineLink()]) -
mean >= stdev * stDevm) {
fileout.println("2.4 Seg length ending at pt. " +
LineArray[i].getOrigionalLineNum() + " >= " + stDevm +
" stdevs above mean at " +
myFormatter.format(this.LineArray[i].getDistance(
LineArray[LineArray[i].getLineLink()])) +
". (no action taken)");
fileout.println(" B1 X, Y, Z: " + this.getX(i) + " " +
this.getY(i) + " " + this.getZ(i));
}
}
}
}
firstTimeThrough = false;
}
}
public void badSegLengthMinFixMax(PrintWriter fileout, double min, double max) {
double mean = this.meanSegLength();
double stdev = this.stdevSegLength();
boolean firstTimeThrough = true;
boolean linesRemoved = true;
while (linesRemoved) {
linesRemoved = false;
for (int i = 2; i < NeuronSize; ++i) {
if (this.LineArray[i].getDistance(LineArray[LineArray[i].getLineLink()]) <= min) {
fileout.println("2.5 Seg length ending at pt. " +
this.LineArray[i].getOrigionalLineNum() + " <= " + min +
" at " +
myFormatter.format(this.LineArray[i].getDistance(LineArray[
LineArray[i].getLineLink()])) + ". (removed)");
fileout.println(" A X, Y, Z: " + this.getX(i) + " " +
this.getY(i) + " " + this.getZ(i));
this.removeLine(i);
linesRemoved = true;
}
if (firstTimeThrough) {
if (this.LineArray[i].getDistance(LineArray[LineArray[i].getLineLink()]) >=
max) {
fileout.println("2.4 Seg length ending at pt. " +
LineArray[i].getOrigionalLineNum() + " >= " + max +
" at " +
myFormatter.format(this.LineArray[i].getDistance(
LineArray[LineArray[i].getLineLink()])) +
". (no action taken)");
fileout.println(" B1 X, Y, Z: " + this.getX(i) + " " +
this.getY(i) + " " + this.getZ(i));
}
}
}
firstTimeThrough = false;
}
}
public void badSegLengthMinFixMax(PrintWriter fileout, double min, double max, int type) {
double mean = this.meanSegLength(type);
double stdev = this.stdevSegLength(type);
boolean firstTimeThrough = true;
boolean linesRemoved = true;
while (linesRemoved) {
linesRemoved = false;
for (int i = 2; i < NeuronSize; ++i) {
if ( (LineArray[i].getLineType() == type)) {
if (this.LineArray[i].getDistance(LineArray[LineArray[i].getLineLink()]) <=
min) {
fileout.println("2.5 Seg length ending at pt. " +
this.LineArray[i].getOrigionalLineNum() + " <= " +
min +
" at " +
myFormatter.format(this.LineArray[i].
getDistance(LineArray[LineArray[i].getLineLink()])) +
". (removed)");
fileout.println(" A X, Y, Z: " + this.getX(i) + " " +
this.getY(i) + " " + this.getZ(i));
this.removeLine(i);
linesRemoved = true;
}
if (firstTimeThrough) {
if (this.LineArray[i].getDistance(LineArray[LineArray[i].getLineLink()]) >=
max) {
fileout.println("2.4 Seg length ending at pt. " +
LineArray[i].getOrigionalLineNum() + " >= " + max +
" at " +
myFormatter.format(this.LineArray[i].getDistance(
LineArray[LineArray[i].getLineLink()])) +
". (no action taken)");
fileout.println(" B1 X, Y, Z: " + this.getX(i) + " " +
this.getY(i) + " " + this.getZ(i));
}
}
}
}
firstTimeThrough = false;
}
}
//********************************************************************************************************************
public double meanSegLength() {
double mean = 0.0;
if (this.NeuronSize > 0) {
double sum = 0.0;
for (int i = 2; i < this.NeuronSize; i++) {
sum += this.LineArray[i].getDistance(LineArray[LineArray[i].getLineLink()]);
}
mean = sum / (this.NeuronSize);
}
return mean;
}
public double meanSegLength(int type) {
double mean = 0.0;
if (this.NeuronSize > 0) {
double sum = 0.0;
int count = 0;
for (int i = 2; i < this.NeuronSize; i++) {
if (this.LineArray[i].getLineType() == type) {
sum += this.LineArray[i].getDistance(LineArray[LineArray[i].getLineLink()]);
++count;
}
}
mean = sum / count;
}
return mean;
}
public double stdevSegLength() {
double stdDev = 0.0;
if (this.NeuronSize > 1) {
double meanValue = this.meanSegLength();
double sum = 0.0;
for (int i = 2; i < this.NeuronSize; i++) {
double diff = this.LineArray[i].getDistance(LineArray[LineArray[i].getLineLink()]) -
meanValue;
sum += diff * diff;
}
stdDev = Math.sqrt(sum / (this.NeuronSize - 1));
}
return stdDev;
}
public double stdevSegLength(int type) {
double stdDev = 0.0;
int count = 0;
if (this.NeuronSize > 1) {
double meanValue = this.meanSegLength(type);
double sum = 0.0;
for (int i = 2; i < this.NeuronSize; i++) {
if (this.LineArray[i].getLineType() == type) {
double diff = this.LineArray[i].getDistance(LineArray[LineArray[i].
getLineLink()]) - meanValue;
sum += diff * diff;
++count;
}
}
stdDev = Math.sqrt(sum / (count - 1));
}
return stdDev;
}
public void SetPatternOut(String SPO) {
PatternOut = SPO;
}
//find 2.1
public void findTrifurcations(PrintWriter fileout) {
if (this.NeuronSize > 0) {
for (int i = 1; i < this.NeuronSize; i++) {
if (this.LineArray[i].getNumDaughters() > 2) {
fileout.println("2.1 Line " + LineArray[i].getOrigionalLineNum() +
" has " + this.LineArray[i].getNumDaughters() +
" daughters. Type is " +
LineArray[i].getLineType() + ". (no action taken)");
fileout.println(" B1 X, Y, Z: " + this.getX(i) + " " +
this.getY(i) + " " + this.getZ(i));
}
}
}
}
//find 2.3
public void badLinks(PrintWriter fileout) {
if (this.NeuronSize > 0) {
for (int i = 2; i < this.NeuronSize; i++) {
if ( (this.getLink(i) >= i) || (this.getLink(i) < -1)) {
fileout.println("2.3 Line " + LineArray[i].getOrigionalLineNum() +
" links to " +
LineArray[this.getLink(i)].getOrigionalLineNum() +
". Last line num is " + this.NeuronSize + " Type is " +
LineArray[i].getLineType() + ". (no action taken)");
fileout.println(" B1 X, Y, Z: " + this.getX(i) + " " +
this.getY(i) + " " + this.getZ(i));
}
}
}
}
//find 4.4
public void badPositiveTaper(PrintWriter fileout, double ratiolimit) {
if (this.NeuronSize > 0) {
for (int i = 2; i < this.NeuronSize; i++) {
if ( (this.getRad(i) / this.getRad(this.getLink(i))) >= ratiolimit) {
fileout.println("4.4 Line " + LineArray[i].getOrigionalLineNum() +
" of rad " + this.getRad(i) + " links to " +
LineArray[this.getLink(i)].getOrigionalLineNum() +
" of rad " + this.getRad(this.getLink(i)) +
". (no action taken)");
fileout.println(" B1 X, Y, Z: " + this.getX(i) + " " +
this.getY(i) + " " + this.getZ(i));
}
}
}
}
//find 4.4
public void badNegativeTaper(PrintWriter fileout, double ratiolimit) {
if (this.NeuronSize > 0) {
for (int i = 2; i < this.NeuronSize; i++) {
if ( (this.getRad(this.getLink(i)) / this.getRad(i)) >= ratiolimit) {
fileout.println("4.4 Line " + LineArray[i].getOrigionalLineNum() +
" of rad " + this.getRad(i) + " links to " +
LineArray[this.getLink(i)].getOrigionalLineNum() +
" of rad " + this.getRad(this.getLink(i)) +
". (no action taken)");
fileout.println(" B1 X, Y, Z: " + this.getX(i) + " " +
this.getY(i) + " " + this.getZ(i));
}
}
}
}
//find 2.7
public void includedSideBranch(PrintWriter fileout) {
if (this.NeuronSize > 0) {
for (int i = 2; i < this.NeuronSize; i++) {
if ( (this.getRad(i) >=
this.LineArray[i].getDistance(LineArray[LineArray[i].getLineLink()]) &&
(this.LineArray[i].getNumDaughters() == 0) &&
(this.LineArray[this.LineArray[i].getLineLink()].getNumDaughters() == 2))) {
fileout.println("2.7 Line " + LineArray[i].getOrigionalLineNum() +
" of rad " + this.getRad(i) +
" is an included side branch. (no action taken)");
fileout.println(" B1 X, Y, Z: " + this.getX(i) + " " +
this.getY(i) + " " + this.getZ(i));
}
}
}
}
//find 2.7
public void includedSideBranchFix(PrintWriter fileout) {
if (this.NeuronSize > 0) {
for (int i = 2; i < this.NeuronSize; i++) {
if ( (this.getRad(i) >=
this.LineArray[i].getDistance(LineArray[LineArray[i].getLineLink()]) &&
(this.LineArray[i].getNumDaughters() == 0) &&
(this.LineArray[this.LineArray[i].getLineLink()].getNumDaughters() == 2))) {
fileout.println("2.7 Line " + LineArray[i].getOrigionalLineNum() +
" of rad " + this.getRad(i) +
" is an included side branch. (removed)");
fileout.println(" A X, Y, Z: " + this.getX(i) + " " +
this.getY(i) + " " + this.getZ(i));
this.removeLine(i);
}
}
}
}
//find 2.7
public void includedSideBranch(PrintWriter fileout, int typeToDo) {
if (this.NeuronSize > 0) {
for (int i = 2; i < this.NeuronSize; i++) {
if (this.getType(i) == typeToDo) {
if ( (this.getRad(i) >=
this.LineArray[i].getDistance(LineArray[LineArray[i].getLineLink()]) &&
(this.LineArray[i].getNumDaughters() == 0) &&
(this.LineArray[this.LineArray[i].getLineLink()].getNumDaughters() ==
2))) {
fileout.println("2.7 Line " + LineArray[i].getOrigionalLineNum() +
" of rad " + this.getRad(i) +
" is an included side branch. (no action taken)");
fileout.println(" B1 X, Y, Z: " + this.getX(i) + " " +
this.getY(i) + " " + this.getZ(i));
}
}
}
}
}
//find 2.7
public void includedSideBranchFix(PrintWriter fileout, int typeToDo) {
if (this.NeuronSize > 0) {
for (int i = 2; i < this.NeuronSize; i++) {
if (this.getType(i) == typeToDo) {
if ( (this.getRad(i) >=
this.LineArray[i].getDistance(LineArray[LineArray[i].getLineLink()]) &&
(this.LineArray[i].getNumDaughters() == 0) &&
(this.LineArray[this.LineArray[i].getLineLink()].getNumDaughters() ==
2))) {
fileout.println("2.7 Line " + LineArray[i].getOrigionalLineNum() +
" of rad " + this.getRad(i) +
" is an included side branch. (removed)");
fileout.println(" A X, Y, Z: " + this.getX(i) + " " +
this.getY(i) + " " + this.getZ(i));
this.removeLine(i);
}
}
}
}
}
//find 2.6
public void overlappingPoints(PrintWriter fileout) {
if (this.NeuronSize > 0) {
for (int i = 1; i < this.NeuronSize; i++) {
for (int j = i + 1; j < this.NeuronSize; j++) {
if ( (this.getX(j) == this.getX(i)) && (this.getY(j) == this.getY(i)) &&
(this.getZ(j) == this.getZ(i))) {
fileout.println("2.6 Line " + LineArray[i].getOrigionalLineNum() +
" and line " + LineArray[j].getOrigionalLineNum() +
" have the same coordinates. (no action taken)");
fileout.println(" B1 X, Y, Z: " + this.getX(i) + " " +
this.getY(i) + " " + this.getZ(i));
}
}
}
}
}
//find 3.3
public void badSoma(PrintWriter fileout) {
if (this.NeuronSize > 0) {
this.setDaughterNums();
int numsomadaughters = 0;
for (int i = 2; i < this.NeuronSize; i++) {
if (this.getType(i) == 1) {
if (this.LineArray[i].getNumDaughters() >= 2) {
for (int j = i; j < this.NeuronSize; j++) {
if (this.getLink(j) == i && this.getType(j) == 1) {
++numsomadaughters;
}
}
if (numsomadaughters >= 2) {
fileout.println("3.3 Soma at line " +
LineArray[i].getOrigionalLineNum() +
" has 2 or more daughters also of type 1. (no action taken)");
fileout.println(" B1 X, Y, Z: " + this.getX(i) + " " +
this.getY(i) + " " + this.getZ(i));
}
}
}
}
}
}
public void setDaughterNums() {
if (this.NeuronSize > 0) {
for (int i = 1; i < this.NeuronSize; i++) {
this.LineArray[i].setNumDaughters(0);
}
for (int i = 2; i < this.NeuronSize; i++) {
this.LineArray[this.getLink(i)].setNumDaughters(this.LineArray[this.getLink(i)].
getNumDaughters() + 1);
}
}
}
public void moveEndLineToBeginning() {
if (this.NeuronSize > 0) {
SwcLine tempLine = new SwcLine();
tempLine = this.LineArray[1];
this.LineArray[1] = this.LineArray[this.NeuronSize];
this.LineArray[1].setLineNum(1);
this.LineArray[1].setLineLink( -1);
for (int i = 2; i < this.NeuronSize - 1; i++) {
this.LineArray[i] = tempLine;
this.LineArray[i].setLineNum(i);
this.LineArray[i].setLineLink(this.getLink(i) + 1);
tempLine = this.LineArray[i + 1];
}
this.LineArray[this.NeuronSize] = tempLine;
this.LineArray[this.NeuronSize].setLineNum(this.NeuronSize);
this.LineArray[this.NeuronSize].setLineLink(this.getLink(this.NeuronSize) + 1);
}
}
public void removeLine(int lineToDel) {
if (this.NeuronSize > 0) {
for (int i = 2; i < this.NeuronSize; i++) {
if (i >= lineToDel) {
int tempLink = this.LineArray[i].getLineLink();
this.LineArray[i] = this.LineArray[i + 1];
this.LineArray[i].setLineNum(i);
if (this.LineArray[i].getLineLink() >= lineToDel) {
this.LineArray[i].setLineLink(this.LineArray[i].getLineLink() - 1);
}
if (i == lineToDel) {
if (tempLink < lineToDel - 1) {
this.LineArray[i].setLineLink(tempLink);
}
}
}
}
this.LineArray[this.NeuronSize] = null;
this.NeuronSize = this.NeuronSize - 1;
}
}
public void removeLines(int firstToDel, int lastToDel) {
if (this.NeuronSize > 0) {
for (int i = lastToDel; i >= firstToDel; i--) {
this.removeLine(i);
}
}
}
public int countType(int typeToCount) {
int count = 0;
if (this.NeuronSize > 0) {
for (int i = 2; i < this.NeuronSize; i++) {
if (this.LineArray[i].getLineType() == typeToCount) {
++count;
}
}
}
return count;
}
public int getFirstDaughterNumType(int lineArraySub) {
if (this.NeuronSize > 0) {
for (int i = 1; i < this.NeuronSize; i++) {
if (this.LineArray[i].getLineLink() == lineArraySub) {
return this.LineArray[i].getLineType();
}
}
}
return -1;
}
//output swc file into given print stream, header information added
public void writeSWC(PrintWriter swcOut, double version) {
if (this.NeuronSize > 0) {
swcOut.println("# Origional file " + this.FileName + " edited using SWCfix version " +
version + ". See SWCfix" + version + ".doc for more information.");
swcOut.println("#");
for (int i = 1; i <= this.CommentSize; i++) {
swcOut.println(CommentLineArray[i]);
}
for (int i = 1; i <= this.NeuronSize; i++) {
swcOut.println(i + " " + this.getType(i) + " " + this.getX(i) + " " +
this.getY(i) + " " + this.getZ(i) + " " + this.getRad(i) + " " +
this.getLink(i));
for (int j = 0; j < 5000; ++j) {
}
}
}
}
//output swc file into given print stream, extensive header information added
public void writeSWC(PrintWriter swcOut, double version, String todaysDate, String userName,
String errorFile) {
if (this.NeuronSize > 0) {
swcOut.println("# Original file " + this.FileName + " edited by " + userName +
" using SWCfix version " + version + " on " + todaysDate + ".");
swcOut.println("# Errors and fixes documented in " + errorFile + ". See SWCfix" +
version + ".doc for more information.");
swcOut.println("#");
for (int i = 1; i <= this.CommentSize; i++) {
swcOut.println(CommentLineArray[i]);
}
for (int i = 1; i <= this.NeuronSize; i++) {
swcOut.println(i + " " + this.getType(i) + " " + this.getX(i) + " " +
this.getY(i) + " " + this.getZ(i) + " " + this.getRad(i) + " " +
this.getLink(i));
for (int j = 0; j < 5000; ++j) {
}
}
}
}
public void setLengths() {
if (this.NeuronSize > 0) {
for (int i = 2; i <= this.NeuronSize; i++) {
//set seg length as the distance between current point and parrent poing
// this.LineArray[i].setSegLength(this.LineArray[i].getDistance(this.LineArray[this.getLink(i)]));
//already donein (InitSwcNeuron)
//set tree lenght to seg length if parretn is soma, else add it to prev
if (this.getType(this.getLink(i)) == 1) {
this.LineArray[i].setTreeLength(this.LineArray[i].getSegLength());
}
else {
this.LineArray[i].setTreeLength(this.LineArray[i].getSegLength() +
this.LineArray[this.getLink(i)].getTreeLength());
}
//set branch length to seg if parrent is bif or soma, else add it to prev
if ( (this.LineArray[this.getLink(i)].getNumDaughters() == 2) ||
(this.getType(this.getLink(i)) == 1)) {
this.LineArray[i].setBranchLength(this.LineArray[i].getSegLength());
// this.LineArray[i].setBranchLength(5);
}
else {
this.LineArray[i].setBranchLength(this.LineArray[i].getSegLength() +
this.LineArray[this.getLink(i)].getBranchLength());
}
//reset all soma point tree and branch lengths
if (this.getType(i) == 1) {
this.LineArray[i].setTreeLength(0);
this.LineArray[i].setBranchLength(0);
}
}
}
}
//output swc file into given print stream, no header information added
public void writeSWC(PrintWriter swcOut) {
if (this.NeuronSize > 0) {
for (int i = 1; i <= this.CommentSize; i++) {
swcOut.println(CommentLineArray[i]);
}
for (int i = 1; i <= this.NeuronSize; i++) {
swcOut.println(i + " " + this.getType(i) + " " + this.getX(i) + " " +
this.getY(i) + " " + this.getZ(i) + " " + this.getRad(i) + " " +
this.getLink(i));
for (int j = 0; j < 5000; ++j) {
}
}
}
}
//temp to output branch order info
public void writeBO(PrintWriter boOut) {
System.out.println("printing branch order");
if (this.NeuronSize > 0) {
for (int i = 1; i <= this.NeuronSize; i++) {
boOut.println(" bo " + this.getBranchOrder(i));
System.out.println(i);
}
}
}
public void setEuclidianDistancesToSoma() {
if (this.NeuronSize > 0) {
//for each line in the cell
for (int i = 2; i <= this.NeuronSize; i++) {
if (this.LineArray[i].getLineType() != 1) {
//System.out.println(i + " " +this.LineArray[i].getStemNumber());
this.LineArray[i].setEuclidianDistance(this.LineArray[i].getDistance(this.
LineArray[this.LineArray[i].getStemNumber()]));
}
}
}
}
public void setStemsStartRadAndBO() {
boolean BranchStartNotSet = true;
if (this.NeuronSize > 1) {
//for each line in the cell
// this.LineArray[2].setStemNumber(1);
for (int i = 2; i <= this.NeuronSize; i++) {
BranchStartNotSet = true;
this.LineArray[i].setBranchOrder(0);
if (this.LineArray[this.LineArray[i].getLineLink()].getLineType() == 1) {
this.LineArray[i].setStemNumber(this.LineArray[i].getLineLink());
this.LineArray[i].setBranchOrder(0);
BranchStartNotSet = false;
this.LineArray[i].setBranchStartRad(this.getRad(i));
}
else {
//go recursively back through cell to soma
for (int k = this.LineArray[i].getLineLink();
this.LineArray[k].getLineType() != 1;
k = this.LineArray[k].getLineLink()) {
//update stem number
this.LineArray[i].setStemNumber(this.LineArray[k].getLineLink());
//update bo if bifurcation encountered
if ( (this.getNumDaughters(k) == 2) && (this.getType(k) != 1)) {
this.LineArray[i].setBranchOrder(this.LineArray[i].
getBranchOrder() + 1);
if (BranchStartNotSet) { //if imediate parrent is bifurcation
BranchStartNotSet = false;
//set start rad to current radius
this.LineArray[i].setBranchStartRad(this.getRad(i));
}
}
//if parent is bifurcation set rad to current radius
if ( (this.getNumDaughters(this.getLink(k)) == 2) &&
BranchStartNotSet) {
BranchStartNotSet = false;
this.LineArray[i].setBranchStartRad(this.getRad(k));
}
//if parrent is soma, set start rad to current point
if ( (this.getType(this.getLink(k)) == 1) && BranchStartNotSet) {
BranchStartNotSet = false;
this.LineArray[i].setBranchStartRad(this.getRad(k));
}
}
}
}
}
}
public int getTreeSize(int k) {
int temp = 0;
while (this.getNumDaughters(k) == 1) {
k = this.getDaughter1num(k);
}
if (this.getNumDaughters(k) == 0) {
return 0;
}
if (this.getNumDaughters(k) == 2) {
temp = 1 + this.getTreeSize(this.getDaughter1num(k)) +
this.getTreeSize(this.getDaughter2num(k));
// System.out.println(k);
return temp;
}
return 0;
}
public void setAllLineTreeNumber() {
int lineNum = 0;
if (this.NeuronSize > 1) {
for (int i = 2; i <= this.NeuronSize; i++) {
if (this.getType(this.getLink(i)) == 1) { //if parrent is soma
++lineNum;
this.setLineTreeNumbers(i, lineNum);
}
}
}
}
public void setLineTreeNumbers(int k, int lineNum) { //set all points in a tree to lineNum
this.LineArray[k].setLineTreeNum(lineNum);
if (this.getNumDaughters(k) == 1) {
this.setLineTreeNumbers(this.getDaughter1num(k), lineNum);
}
if (this.getNumDaughters(k) == 2) {
this.setLineTreeNumbers(this.getDaughter1num(k), lineNum);
this.setLineTreeNumbers(this.getDaughter2num(k), lineNum);
}
}
public int getTreeSize(int k, int type) {
int temp = 0;
while (this.getNumDaughters(k) == 1) {
k = this.getDaughter1num(k);
if (this.getType(k) != type) {
return 0;
}
}
if (this.getNumDaughters(k) == 0) {
return 0;
}
if (this.getNumDaughters(k) == 2) {
if ( (this.getType(this.getDaughter1num(k)) != type) &&
(this.getType(this.getDaughter1num(k)) != type)) {
temp = 0;
}
else if (this.getType(this.getDaughter1num(k)) != type) {
temp = this.getTreeSize(this.getDaughter2num(k), type);
}
else if (this.getType(this.getDaughter2num(k)) != type) {
temp = this.getTreeSize(this.getDaughter1num(k), type);
}
else {
temp = 1 + this.getTreeSize(this.getDaughter1num(k), type) +
this.getTreeSize(this.getDaughter2num(k), type);
}
// System.out.println("biff " + k);
return temp;
}
return 0;
}
}