//**************************************************************************************************
objref Hcell[nhcell]
begintemplate HIPPCell
ndend1=3
ndend2=3
ndend3=3
ndend4=3
public pre_list, connect_pre, subsets, is_art, is_connected
public vbc2gc, vmc2gc, vhc2gc, vgc2bc, vbc2bc, vmc2bc, vhc2bc, vgc2mc, vbc2mc, vmc2mc, vhc2mc, vgc2hc, vmc2hc
public soma, hcdend1, hcdend2, hcdend3, hcdend4
create soma, hcdend1[ndend1], hcdend2[ndend2], hcdend3[ndend3], hcdend4[ndend4]
public all, pdend, ddend
objref syn, pre_list
nst=10
objectvar stim[nst]
double stimdur[nst], stimdel[nst], stimamp[nst]
public stim, stimdur, stimamp, stimdel
objref syn
proc init() {
pre_list = new List()
subsets()
temp()
synapse()
}
objref all, pdend, ddend
proc subsets() { local i
objref all, pdend, ddend
all = new SectionList()
soma all.append()
for i=0, 2 hcdend1 [i] all.append()
for i=0, 2 hcdend2 [i] all.append()
for i=0, 2 hcdend3 [i] all.append()
for i=0, 2 hcdend4 [i] all.append()
pdend = new SectionList()
hcdend1 [0] pdend.append()
hcdend2 [0] pdend.append()
hcdend3 [0] pdend.append()
hcdend4 [0] pdend.append()
ddend = new SectionList()
for i=1, 2 hcdend1 [i] ddend.append()
for i=1, 2 hcdend2 [i] ddend.append()
for i=1, 2 hcdend3 [i] ddend.append()
for i=1, 2 hcdend4 [i] ddend.append()
}
proc temp() {
soma {nseg=1 L=20 diam=10} // changed L & diam
hcdend1 [0] {nseg=1 L=75 diam=3}
hcdend1 [1] {nseg=1 L=75 diam=2}
hcdend1 [2] {nseg=1 L=75 diam=1}
hcdend2 [0] {nseg=1 L=75 diam=3}
hcdend2 [1] {nseg=1 L=75 diam=2}
hcdend2 [2] {nseg=1 L=75 diam=1}
hcdend3 [0] {nseg=1 L=50 diam=3}
hcdend3 [1] {nseg=1 L=50 diam=2}
hcdend3 [2] {nseg=1 L=50 diam=1}
hcdend4 [0] {nseg=1 L=50 diam=3}
hcdend4 [1] {nseg=1 L=50 diam=2}
hcdend4 [2] {nseg=1 L=50 diam=1}
forall {
insert ccanl
catau_ccanl = 10
caiinf_ccanl = 5.e-6
insert borgka
gkabar_borgka=0.0008
insert nca // HAV-N- Ca channel
gncabar_nca=0.0 //0005 check to modify- original 0.004
insert lca
glcabar_lca=0.0015
insert gskch
gskbar_gskch=0.003
insert cagk
gkbar_cagk=0.003
insert hyperde3
ghyfbar_hyperde3=0.000015
ghysbar_hyperde3=0.000015
}
soma {insert ichan2 //ildikos ichan
gnatbar_ichan2=0.2 //original 0.030 to .055
gkfbar_ichan2=0.006 //original 0.015
gl_ichan2 = 0.000036
cm=1.1}
forsec pdend {insert ichan2
gnatbar_ichan2=0.2 //original 0.015
gkfbar_ichan2=0.006
gl_ichan2 = 0.000036
cm=1.1}
forsec ddend {insert ichan2
gnatbar_ichan2=0.0
gkfbar_ichan2=0.00
gl_ichan2 = 0.000036
cm=1.1}
connect hcdend1[0](0), soma(1)
connect hcdend2[0](0), soma(1)
connect hcdend3[0](0), soma(0)
connect hcdend4[0](0), soma(0)
for i=1,2 {connect hcdend1[i](0), hcdend1[i-1](1)}
for i=1,2 {connect hcdend2[i](0), hcdend2[i-1](1)}
for i=1,2 {connect hcdend3[i](0), hcdend3[i-1](1)}
for i=1,2 {connect hcdend4[i](0), hcdend4[i-1](1)}
forall {Ra=100}
forall {enat = 55 ekf = -90 ek=-90 esk=-90 elca=130
el_ichan2 =-70.45 ehyf=-40 ehys=-40
cao_ccanl=2 } // make catau slower70e-3 cao=2 cai=50.e-6
//for i=0,0 {
//stimdel[i]=500
//stimdur[i]=500
//stimamp[i]=0.1
/* 0.4 stim when we want the cell to fire with regular spikes */
//soma stim[i] = new IClamp(0.5)
//stim.del[i]=stimdel[i]
//stim.dur[i]=stimdur[i]
//stim.amp[i]=stimamp[i]
//}
}
objref syn
proc synapse() {
hcdend1 [0] syn = new Exp2Syn(0.5) //GC(AMPA) syn to prox dend similar to GC>BC
syn.tau1 = .3 syn.tau2 = .6 syn.e = 0
pre_list.append(syn)
hcdend2 [0] syn = new Exp2Syn(0.5) //GC(AMPA) syn to prox dend similar to GC>BC
syn.tau1 = .3 syn.tau2 = .6 syn.e = 0
pre_list.append(syn)
hcdend3 [0] syn = new Exp2Syn(0.5) //GC(AMPA) syn to prox dend similar to GC>BC
syn.tau1 = .3 syn.tau2 = .6 syn.e = 0
pre_list.append(syn)
hcdend4 [0] syn = new Exp2Syn(0.5) //GC(AMPA) syn to prox dend similar to GC>BC
syn.tau1 = .3 syn.tau2 = .6 syn.e = 0
pre_list.append(syn)
hcdend1 [1] syn = new Exp2Syn(0.5) //MC(AMPA) syn to mid dend similar to CA3>int Aaron
syn.tau1 = .9 syn.tau2 = 3.6 syn.e = 0 //*** Assumed data at physio temp
pre_list.append(syn)
hcdend2 [1] syn = new Exp2Syn(0.5) //MC(AMPA) syn to mid dend similar to CA3>int Aaron
syn.tau1 = 0.9 syn.tau2 = 3.6 syn.e = 0 //*** Assumed data at physio temp
pre_list.append(syn)
hcdend3 [1] syn = new Exp2Syn(0.5) //MC(AMPA) syn to mid dend similar to CA3>int Aaron
syn.tau1 = 0.9 syn.tau2 = 3.6 syn.e = 0 //*** Assumed data at physio temp
pre_list.append(syn)
hcdend4 [1] syn = new Exp2Syn(0.5) //MC(AMPA) syn to mid dend similar to CA3>int Aaron
syn.tau1 = 0.9 syn.tau2 = 3.6 syn.e = 0 //*** Assumed data at physio temp
pre_list.append(syn)
// Total of 12 synapses 0-3 PP; 4-7 GC; 8-11 MC
}
proc connect_pre() { // $o1 target point process, $o2 returned NetCon
soma $o2 = new NetCon (&v(1), $o1)
}
func is_art() { return 0 }
endtemplate HIPPCell
//************************************************************************************************************