{load_file("nrngui.hoc")} // load the GUI and standard run libraries
{load_file("../cells/axoaxonic_cell.hoc")} //load axoaxonic cell
{xopen("kblock.hoc")} //execute open.load_file just reads
strdef temp_mkdir, temp_dir
// Create the directory
sprint(temp_dir, "SynapticProperties/")
sprint(temp_mkdir, "mkdir -p %s", temp_dir)
system(temp_mkdir)
objectvar Cell1, Cell2, Cell3, Cell4, Cell5, Cell6, Cell7
objectvar syn1, syn2, syn3, syn4, syn5, syn6, syn7
objectvar nclist, stim, sn1, sn2, sn3, sn4, sn5, sn6, sn7
objectvar clamp1, clamp2, clamp3, clamp4, clamp5, clamp6, clamp7
objref sn1, i1_ca1, i2_ca1, i3_ca1, i4_ca1, i5_ca1, i6_ca1, i7_ca1
objref FILE1, FILE2, FILE3, FILE4, FILE5, FILE6, FILE7
strdef tmp1, tmp2, tmp3, tmp4, tmp5, tmp6, tmp7
Cell1 = new AACell() // template for synapse EC to AAC
Cell2 = new AACell() // template for synapse CA3 to AAC
Cell3 = new AACell() // template for synapse PC to AAC
Cell4 = new AACell() // template for synapse BC to AAC
Cell5 = new AACell() // template for synapse BSC to AAC
Cell6 = new AACell() // template for synapse OLM to AAC
Cell7 = new AACell() // template for synapse VIPCCK to AAC
stim = new NetStim (0.5)
stim.interval = 1
stim.number = 1
stim.start = 900
// Time parameters///////////////////////////////
tstop = 2000 //stop time in ms
steps_per_ms = 10
dt = 0.1
n = tstop*steps_per_ms
//Vclamp for AMPA & GABAA
print "Vclamp"
factor2 = 1.0
//making EC synapse to AAC at lmM1 dendrite
//weigth_ECtoAAC
w1 = 1.2e-4
Cell1.lmM1 syn1 = new MyExp2Syn(0.5)
syn1.tau1 = 2.0
syn1.tau2 = 6.3
syn1.e = 0
//making the netcon
sn1 = new NetCon(stim, syn1, -20, 1, w1)
//making CA3 synapse to AAC at radMed1 dendrite
//weigth_CA3toAAC
w2 = 1.2e-4
Cell2.radMed1 syn2 = new MyExp2Syn(0.5)
syn2.tau1 = 2.0
syn2.tau2 = 6.3
syn2.e = 0
//making the netcon
sn2 = new NetCon (stim,syn2,-20, 1, w2) //..threshold,delay,weight)
//making PC synapse to AAC at oriProx1 dendrite
//weigth_PCtoAAC
w3 = 4.0e-5
Cell3.oriProx1 syn3 = new MyExp2Syn(0.5)
syn3.tau1 = 0.3
syn3.tau2 = 0.6
syn3.e = 0
//making the netcon
sn3 = new NetCon(stim, syn3, -20, 1, w3)
//making BC synapse to AAC at soma
//weigth_BCtoAAC
w4 = 1.2e-4*factor2
Cell4.soma syn4 = new MyExp2Syn(0.5)
syn4.tau1 = 0.29
syn4.tau2 = 2.67
syn4.e = -75
//making the netcon
sn4 = new NetCon(stim, syn4, -20, 1, w4)
//making BSC synapse to AAC at radMed1 dendrite
//weigth_BSCtoAAC
w5 = 6.0e-4*factor2
Cell5.radMed1 syn5 = new MyExp2Syn(0.5)
syn5.tau1 = 0.29
syn5.tau2 = 2.67
syn5.e = -75
//making the netcon
sn5 = new NetCon(stim, syn5, -20, 1, w5)
//making OLM synapse to AAC at lmM1 dendrite
//weigth_OLMtoAAC
w6 = 1.2e-4*factor2
Cell6.lmM1 syn6 = new MyExp2Syn(0.5)
syn6.tau1 = 0.73
syn6.tau2 = 10.0
syn6.e = -75
//making the netcon
sn6 = new NetCon(stim, syn6, -20, 1, w6)
//making VIPCCK synapse to AAC at soma
//weigth_VIPCCKtoAAC
w7 = 7.0e-4*factor2
Cell7.soma syn7 = new MyExp2Syn(0.5)
syn7.tau1 = 0.43
syn7.tau2 = 4.49
syn7.e = -75
//making the netcon
sn7 = new NetCon(stim, syn7, -20, 1, w7)
//Vclamp at soma
Cell1.soma clamp1 = new VClamp(0.5)
Cell2.soma clamp2 = new VClamp(0.5)
Cell3.soma clamp3 = new VClamp(0.5)
Cell4.soma clamp4 = new VClamp(0.5)
Cell5.soma clamp5 = new VClamp(0.5)
Cell6.soma clamp6 = new VClamp(0.5)
Cell7.soma clamp7 = new VClamp(0.5)
clamp1.dur[0]= 2000 //duration of clamp in ms
clamp1.amp[0]= -64.42 //voltage clamp amplitude / near to Vrest
clamp2.dur[0]= 2000
clamp2.amp[0]= -64.42
clamp3.dur[0]= 2000
clamp3.amp[0]= -64.42
clamp4.dur[0]= 2000
clamp4.amp[0]= -64.42
clamp5.dur[0]= 2000
clamp5.amp[0]= -64.42
clamp6.dur[0]= 2000
clamp6.amp[0]= -64.42
clamp7.dur[0]= 2000
clamp7.amp[0]= -64.42
//cutting potasium
//k_block()
//making the vectors
i1_ca1 = new Vector(n)
i2_ca1 = new Vector(n)
i3_ca1 = new Vector(n)
i4_ca1 = new Vector(n)
i5_ca1 = new Vector(n)
i6_ca1 = new Vector(n)
i7_ca1 = new Vector(n)
//recording
i1_ca1.record(&clamp1.i)
i2_ca1.record(&clamp2.i)
i3_ca1.record(&clamp3.i)
i4_ca1.record(&clamp4.i)
i5_ca1.record(&clamp5.i)
i6_ca1.record(&clamp6.i)
i7_ca1.record(&clamp7.i)
run()
FILE1 = new File()
sprint(tmp1, "SynapticProperties/ECtoAAC_i_rec_soma.txt")
FILE1.wopen(tmp1)
for i=0, i1_ca1.size()-1 {FILE1.printf("%f\n",i1_ca1.x[i])}
FILE1.close()
FILE2 = new File()
sprint(tmp2, "SynapticProperties/CA3toAAC_i_rec_soma.txt")
FILE2.wopen(tmp2)
for i=0, i2_ca1.size()-1 {FILE2.printf("%f\n",i2_ca1.x[i])}
FILE2.close()
FILE3 = new File()
sprint(tmp3, "SynapticProperties/PCtoAAC_i_rec_soma.txt")
FILE3.wopen(tmp3)
for i=0, i3_ca1.size()-1 {FILE3.printf("%f\n",i3_ca1.x[i])}
FILE3.close()
FILE4 = new File()
sprint(tmp4, "SynapticProperties/BCtoAAC_i_rec_soma.txt")
FILE4.wopen(tmp4)
for i=0, i4_ca1.size()-1 {FILE4.printf("%f\n",i4_ca1.x[i])}
FILE4.close()
FILE5 = new File()
sprint(tmp5, "SynapticProperties/BSCtoAAC_i_rec_soma.txt")
FILE5.wopen(tmp5)
for i=0, i5_ca1.size()-1 {FILE5.printf("%f\n",i5_ca1.x[i])}
FILE5.close()
FILE6 = new File()
sprint(tmp6, "SynapticProperties/OLMtoAAC_i_rec_soma.txt")
FILE6.wopen(tmp6)
for i=0, i6_ca1.size()-1 {FILE6.printf("%f\n",i6_ca1.x[i])}
FILE6.close()
FILE7 = new File()
sprint(tmp7, "SynapticProperties/VIPCCKtoAAC_i_rec_soma.txt")
FILE7.wopen(tmp7)
for i=0, i7_ca1.size()-1 {FILE7.printf("%f\n",i7_ca1.x[i])}
FILE7.close()