{load_file("nrngui.hoc")} // load the GUI and standard run libraries
{load_file("../cells/basket_cell.hoc")} //load axoaxonic cell
{xopen("kblock.hoc")} //execute open.load_file just reads
strdef temp_mkdir, temp_dir
// Create the directory
sprint(temp_dir, "SynapticProperties/")
sprint(temp_mkdir, "mkdir -p %s", temp_dir)
system(temp_mkdir)
objectvar Cell1, Cell2, Cell3, Cell4, Cell5, Cell6, Cell7
objectvar syn1, syn2, syn3, syn4, syn5, syn6, syn7
objectvar nclist, stim, sn1, sn2, sn3, sn4, sn5, sn6, sn7
objectvar clamp1, clamp2, clamp3, clamp4, clamp5, clamp6, clamp7
objref sn1, i1_ca1, i2_ca1, i3_ca1, i4_ca1, i5_ca1, i6_ca1, i7_ca1
objref FILE1, FILE2, FILE3, FILE4, FILE5, FILE6, FILE7
strdef tmp1, tmp2, tmp3, tmp4, tmp5, tmp6, tmp7
Cell1 = new BasketCell() // template for synapse EC to BC
Cell2 = new BasketCell() // template for synapse CA3 to BC
Cell3 = new BasketCell() // template for synapse PC to BC
Cell4 = new BasketCell() // template for synapse BC to BC
Cell5 = new BasketCell() // template for synapse BSC to BC
Cell6 = new BasketCell() // template for synapse OLM to BC
Cell7 = new BasketCell() // template for synapse VIPCR to BC
stim = new NetStim (0.5)
stim.interval = 1
stim.number = 1
stim.start = 900
// Time parameters///////////////////////////////
tstop = 2000 //stop time in ms
steps_per_ms = 10
dt = 0.1
n = tstop*steps_per_ms
//Vclamp for AMPA & GABAA
print "Vclamp"
factor2 = 1.0
//making EC synapse to BC at lmM1 dendrite
//weigth_ECtoBC
w1 = 0.1e-4
Cell1.lmM1 syn1 = new MyExp2Syn(0.5)
syn1.tau1 = 2.0
syn1.tau2 = 6.3
syn1.e = 0
//making the netcon
sn1 = new NetCon(stim, syn1, -20, 1, w1)
//making CA3 synapse to BC at radMed1 dendrite
//weigth_CA3toBC
w2 = 2.2e-4
Cell2.radMed1 syn2 = new MyExp2Syn(0.5)
syn2.tau1 = 2.0
syn2.tau2 = 6.3
syn2.e = 0
//making the netcon
sn2 = new NetCon (stim,syn2,-20, 1, w2) //..threshold,delay,weight)
//making PC synapse to BC at oriProx1 dendrite
//weigth_PCtoBC
w3 = 7.0e-4
Cell3.oriProx1 syn3 = new MyExp2Syn(0.5)
syn3.tau1 = 0.07
syn3.tau2 = 0.20
syn3.e = 0
//making the netcon
sn3 = new NetCon(stim, syn3, -20, 1, w3)
//making BC synapse to BC at soma
//weigth_BCtoBC
w4 = 1.6e-3*factor2
Cell4.soma syn4 = new MyExp2Syn(0.5)
syn4.tau1 = 0.08
syn4.tau2 = 4.8
syn4.e = -75
//making the netcon
sn4 = new NetCon(stim, syn4, -20, 1, w4)
//making BSC synapse to BC at radMed1 dendrite
//weigth_BSCtoBC
w5 = 9.0e-3*factor2
Cell5.radMed1 syn5 = new MyExp2Syn(0.5)
syn5.tau1 = 0.29
syn5.tau2 = 2.67
syn5.e = -75
//making the netcon
sn5 = new NetCon(stim, syn5, -20, 1, w5)
//making OLM synapse to BC at lmM1 dendrite
//weigth_OLMtoBC
w6 = 1.1e-3*factor2
Cell6.lmM1 syn6 = new MyExp2Syn(0.5)
syn6.tau1 = 0.25
syn6.tau2 = 7.50
syn6.e = -75
//making the netcon
sn6 = new NetCon(stim, syn6, -20, 1, w6)
//making VIPCR synapse to BC at soma
//weigth_VIPCRtoBC
w7 = 9.0e-3*factor2*5.0
Cell7.soma syn7 = new MyExp2Syn(0.5)
syn7.tau1 = 0.43
syn7.tau2 = 4.49
syn7.e = -75
//making the netcon
sn7 = new NetCon(stim, syn7, -20, 1, w7)
//Vclamp at soma
Cell1.soma clamp1 = new VClamp(0.5)
Cell2.soma clamp2 = new VClamp(0.5)
Cell3.soma clamp3 = new VClamp(0.5)
Cell4.soma clamp4 = new VClamp(0.5)
Cell5.soma clamp5 = new VClamp(0.5)
Cell6.soma clamp6 = new VClamp(0.5)
Cell7.soma clamp7 = new VClamp(0.5)
clamp1.dur[0]= 2000 //duration of clamp in ms
clamp1.amp[0]= -61.11 //voltage clamp amplitude / near to Vrest
clamp2.dur[0]= 2000
clamp2.amp[0]= -61.11
clamp3.dur[0]= 2000
clamp3.amp[0]= -61.11
clamp4.dur[0]= 2000
clamp4.amp[0]= -61.11
clamp5.dur[0]= 2000
clamp5.amp[0]= -61.11
clamp6.dur[0]= 2000
clamp6.amp[0]= -61.11
clamp7.dur[0]= 2000
clamp7.amp[0]= -61.11
//cutting potasium
//k_block()
//making the vectors
i1_ca1 = new Vector(n)
i2_ca1 = new Vector(n)
i3_ca1 = new Vector(n)
i4_ca1 = new Vector(n)
i5_ca1 = new Vector(n)
i6_ca1 = new Vector(n)
i7_ca1 = new Vector(n)
//recording
i1_ca1.record(&clamp1.i)
i2_ca1.record(&clamp2.i)
i3_ca1.record(&clamp3.i)
i4_ca1.record(&clamp4.i)
i5_ca1.record(&clamp5.i)
i6_ca1.record(&clamp6.i)
i7_ca1.record(&clamp7.i)
run()
FILE1 = new File()
sprint(tmp1, "SynapticProperties/ECtoBC_i_rec_soma.txt")
FILE1.wopen(tmp1)
for i=0, i1_ca1.size()-1 {FILE1.printf("%f\n",i1_ca1.x[i])}
FILE1.close()
FILE2 = new File()
sprint(tmp2, "SynapticProperties/CA3toBC_i_rec_soma.txt")
FILE2.wopen(tmp2)
for i=0, i2_ca1.size()-1 {FILE2.printf("%f\n",i2_ca1.x[i])}
FILE2.close()
FILE3 = new File()
sprint(tmp3, "SynapticProperties/PCtoBC_i_rec_soma.txt")
FILE3.wopen(tmp3)
for i=0, i3_ca1.size()-1 {FILE3.printf("%f\n",i3_ca1.x[i])}
FILE3.close()
FILE4 = new File()
sprint(tmp4, "SynapticProperties/BCtoBC_i_rec_soma.txt")
FILE4.wopen(tmp4)
for i=0, i4_ca1.size()-1 {FILE4.printf("%f\n",i4_ca1.x[i])}
FILE4.close()
FILE5 = new File()
sprint(tmp5, "SynapticProperties/BSCtoBC_i_rec_soma.txt")
FILE5.wopen(tmp5)
for i=0, i5_ca1.size()-1 {FILE5.printf("%f\n",i5_ca1.x[i])}
FILE5.close()
FILE6 = new File()
sprint(tmp6, "SynapticProperties/OLMtoBC_i_rec_soma.txt")
FILE6.wopen(tmp6)
for i=0, i6_ca1.size()-1 {FILE6.printf("%f\n",i6_ca1.x[i])}
FILE6.close()
FILE7 = new File()
sprint(tmp7, "SynapticProperties/VIPCRtoBC_i_rec_soma.txt")
FILE7.wopen(tmp7)
for i=0, i7_ca1.size()-1 {FILE7.printf("%f\n",i7_ca1.x[i])}
FILE7.close()