{load_file("nrngui.hoc")} // load the GUI and standard run libraries
{load_file("../cells/olm_cell.hoc")} //load axoaxonic cell
{xopen("kblock.hoc")} //execute open.load_file just reads
strdef temp_mkdir, temp_dir
// Create the directory
sprint(temp_dir, "SynapticProperties/")
sprint(temp_mkdir, "mkdir -p %s", temp_dir)
system(temp_mkdir)
objectvar Cell1, Cell2, Cell3, Cell4, Cell5
objectvar syn1, syn2, syn3, syn4, syn5
objectvar nclist, stim, sn1, sn2, sn3, sn4, sn5
objectvar clamp1, clamp2, clamp3, clamp4, clamp5
objref sn1, i1_ca1, i2_ca1, i3_ca1, i4_ca1, i5_ca1
objref FILE1, FILE2, FILE3, FILE4, FILE5
strdef tmp1, tmp2, tmp3, tmp4, tmp5
Cell1 = new OLMCell() // template for synapse CA3 to OLM
Cell2 = new OLMCell() // template for synapse PC to OLM
Cell3 = new OLMCell() // template for synapse BSC to OLM
Cell4 = new OLMCell() // template for synapse OLM to OLM
Cell5 = new OLMCell() // template for synapse VIPCR to OLM
stim = new NetStim (0.5)
stim.interval = 1
stim.number = 1
stim.start = 900
// Time parameters///////////////////////////////
tstop = 2000 //stop time in ms
steps_per_ms = 10
dt = 0.1
n = tstop*steps_per_ms
//Vclamp for AMPA & GABAA
print "Vclamp"
factor2 = 1.0
//making CA3 synapse to OLM at dend1 dendrite
//weigth_CA3toOLM
w1 = 1.05e-4
Cell1.dend1 syn1 = new MyExp2Syn(0.5)
syn1.tau1 = 2.0
syn1.tau2 = 6.3
syn1.e = 0
//making the netcon
sn1 = new NetCon(stim, syn1, -20, 1, w1)
//making PC synapse to OLM at dend1 dendrite
//weigth_PCtoOLM
w2 = 2.0e-4
Cell2.dend1 syn2 = new MyExp2Syn(0.5)
syn2.tau1 = 0.3
syn2.tau2 = 0.6
syn2.e = 0
//making the netcon
sn2 = new NetCon (stim,syn2,-20, 1, w2) //..threshold,delay,weight)
//making BSC synapse to OLM at dend1 dendrite
//weigth_BSCtoOLM
w3 = 2.0e-5*factor2
Cell3.dend1 syn3 = new MyExp2Syn(0.5)
syn3.tau1 = 1.0
syn3.tau2 = 8.0
syn3.e = -75
//making the netcon
sn3 = new NetCon(stim, syn3, -20, 1, w3)
//making OLM synapse to OLM at dend1 dendrite
//weigth_OLMtoOLM
w4 = 1.2e-3*factor2
Cell4.dend1 syn4 = new MyExp2Syn(0.5)
syn4.tau1 = 0.25
syn4.tau2 = 7.50
syn4.e = -75
//making the netcon
sn4 = new NetCon(stim, syn4, -20, 1, w4)
//making VIPCR synapse to OLM at soma
//weigth_VIPCRtoOLM
w5 = 7.0e-4*factor2*5.0
Cell5.soma syn5 = new MyExp2Syn(0.5)
syn5.tau1 = 1.0
syn5.tau2 = 8.0
syn5.e = -75
//making the netcon
sn5 = new NetCon(stim, syn5, -20, 1, w5)
//Vclamp at soma
Cell1.soma clamp1 = new VClamp(0.5)
Cell2.soma clamp2 = new VClamp(0.5)
Cell3.soma clamp3 = new VClamp(0.5)
Cell4.soma clamp4 = new VClamp(0.5)
Cell5.soma clamp5 = new VClamp(0.5)
clamp1.dur[0]= 2000 //duration of clamp in ms
clamp1.amp[0]= -73.5 //voltage clamp amplitude / near to Vrest
clamp2.dur[0]= 2000
clamp2.amp[0]= -73.5
clamp3.dur[0]= 2000
clamp3.amp[0]= -73.5
clamp4.dur[0]= 2000
clamp4.amp[0]= -73.5
clamp5.dur[0]= 2000
clamp5.amp[0]= -73.5
//cutting potasium
//k_block()
//making the vectors
i1_ca1 = new Vector(n)
i2_ca1 = new Vector(n)
i3_ca1 = new Vector(n)
i4_ca1 = new Vector(n)
i5_ca1 = new Vector(n)
//recording
i1_ca1.record(&clamp1.i)
i2_ca1.record(&clamp2.i)
i3_ca1.record(&clamp3.i)
i4_ca1.record(&clamp4.i)
i5_ca1.record(&clamp5.i)
run()
FILE1 = new File()
sprint(tmp1, "SynapticProperties/CA3toOLM_i_rec_soma.txt")
FILE1.wopen(tmp1)
for i=0, i1_ca1.size()-1 {FILE1.printf("%f\n",i1_ca1.x[i])}
FILE1.close()
FILE2 = new File()
sprint(tmp2, "SynapticProperties/PCtoOLM_i_rec_soma.txt")
FILE2.wopen(tmp2)
for i=0, i2_ca1.size()-1 {FILE2.printf("%f\n",i2_ca1.x[i])}
FILE2.close()
FILE3 = new File()
sprint(tmp3, "SynapticProperties/BSCtoOLM_i_rec_soma.txt")
FILE3.wopen(tmp3)
for i=0, i3_ca1.size()-1 {FILE3.printf("%f\n",i3_ca1.x[i])}
FILE3.close()
FILE4 = new File()
sprint(tmp4, "SynapticProperties/OLMtoOLM_i_rec_soma.txt")
FILE4.wopen(tmp4)
for i=0, i4_ca1.size()-1 {FILE4.printf("%f\n",i4_ca1.x[i])}
FILE4.close()
FILE5 = new File()
sprint(tmp5, "SynapticProperties/VIPCRtoOLM_i_rec_soma.txt")
FILE5.wopen(tmp5)
for i=0, i5_ca1.size()-1 {FILE5.printf("%f\n",i5_ca1.x[i])}
FILE5.close()