{load_file("nrngui.hoc")} // load the GUI and standard run libraries
{load_file("../cells/vipcr_cell.hoc")} //load axoaxonic cell
{xopen("kblock.hoc")} //execute open.load_file just reads
strdef temp_mkdir, temp_dir
// Create the directory
sprint(temp_dir, "SynapticProperties/")
sprint(temp_mkdir, "mkdir -p %s", temp_dir)
system(temp_mkdir)
objectvar Cell1, Cell2, Cell3
objectvar syn1, syn2, syn3
objectvar nclist, stim, sn1, sn2, sn3
objectvar clamp1, clamp2, clamp3
objref sn1, i1_ca1, i2_ca1, i3_ca1
objref FILE1, FILE2, FILE3
strdef tmp1, tmp2, tmp3
Cell1 = new VIPCRCell() // synapse from EC to VIPCR
Cell2 = new VIPCRCell() // synapse from CA3 to VIPCR
Cell3 = new VIPCRCell() // synapse from PC to VIPCR
stim = new NetStim (0.5)
stim.interval = 1
stim.number = 1
stim.start = 900
// Time parameters///////////////////////////////
tstop = 2000 //stop time in ms
steps_per_ms = 10
dt = 0.1
n = tstop*steps_per_ms
//Vclamp for AMPA & GABAA
print "Vclamp"
factor2 = 1.0
//making EC synapse to VIPCR at lmM1 dendrite
//weigth_ECtoVIPCR
w1 = 3.0e-4
Cell1.lmM1 syn1 = new MyExp2Syn(0.5)
syn1.tau1 = 2.0
syn1.tau2 = 6.3
syn1.e = 0
//making the netcon
sn1 = new NetCon(stim, syn1, -20, 1, w1)
//making CA3 synapse to VIPCR at radMed1 dendrite
//weigth_CA3toVIPCR
w2 = 1.05e-4
Cell2.radMed1 syn2 = new MyExp2Syn(0.5)
syn2.tau1 = 2.0
syn2.tau2 = 6.3
syn2.e = 0
//making the netcon
sn2 = new NetCon (stim,syn2,-20, 1, w2) //..threshold,delay,weight)
//making PC synapse to VIPCR at oriProx1 dendrite
//weigth_PCtoVIPCR
w3 = 5.0e-4
Cell3.oriProx1 syn3 = new MyExp2Syn(0.5)
syn3.tau1 = 0.5
syn3.tau2 = 3.0
syn3.e = 0
//making the netcon
sn3 = new NetCon(stim, syn3, -20, 1, w3)
//Vclamp at soma
Cell1.soma clamp1 = new VClamp(0.5)
Cell2.soma clamp2 = new VClamp(0.5)
Cell3.soma clamp3 = new VClamp(0.5)
clamp1.dur[0]= 2000 //duration of clamp in ms
clamp1.amp[0]= -70 //voltage clamp amplitude / near to Vrest
clamp2.dur[0]= 2000
clamp2.amp[0]= -70
clamp3.dur[0]= 2000
clamp3.amp[0]= -70
//cutting potasium
//k_block()
//making the vectors
i1_ca1 = new Vector(n)
i2_ca1 = new Vector(n)
i3_ca1 = new Vector(n)
//recording
i1_ca1.record(&clamp1.i)
i2_ca1.record(&clamp2.i)
i3_ca1.record(&clamp3.i)
run()
FILE1 = new File()
sprint(tmp1, "SynapticProperties/ECtoVIPCR_i_rec_soma.txt")
FILE1.wopen(tmp1)
for i=0, i1_ca1.size()-1 {FILE1.printf("%f\n",i1_ca1.x[i])}
FILE1.close()
FILE2 = new File()
sprint(tmp2, "SynapticProperties/CA3toVIPCR_i_rec_soma.txt")
FILE2.wopen(tmp2)
for i=0, i2_ca1.size()-1 {FILE2.printf("%f\n",i2_ca1.x[i])}
FILE2.close()
FILE3 = new File()
sprint(tmp3, "SynapticProperties/PCtoVIPCR_i_rec_soma.txt")
FILE3.wopen(tmp3)
for i=0, i3_ca1.size()-1 {FILE3.printf("%f\n",i3_ca1.x[i])}
FILE3.close()