: kcnab2.mod codes low-threshold K+ channel Kv1.1 coexpressed with Kvbeta2b/kcnab2b in zebrafish.
: Default parameters of a H-H equation are fitted to our experimental data
: by using our channel generator.
:
: Takaki Watanabe
: wtakaki@m.u-tokyo.ac.jp
UNITS {
(mA) = (milliamp)
(mV) = (millivolt)
(nA) = (nanoamp)
}
NEURON {
SUFFIX kcnab2
USEION k READ ek WRITE ik
RANGE gkcnab2bar, gkcnab2, ik
GLOBAL winf4, zinf4, wtau4, ztau4
GLOBAL aa4,bb4,cc4,dd4,ee4,ff4,gg4,hh4,ii4,jj4,kk4,ll4,mm4,nn4,oo4,pp4,qq4,rr4,ss4
}
INDEPENDENT {t FROM 0 TO 1 WITH 1 (ms)}
PARAMETER {
v (mV)
celsius = 20 (degC)
dt (ms)
ek = -90 (mV)
gkcnab2bar = 0.01592 (mho/cm2) <0,1e9>
aa4= 35 <0,1e3>
bb4= 9 <0,1e3>
cc4= 0.25 <0,1e3>
dd4= 71 <0,1e3>
ee4= 150 <0,1e3>
ff4= 175 <0,1e3>
gg4= 6 <0,1e3>
hh4= 85 <0,1e3>
ii4= 20 <0,1e3>
jj4= 80 <0,1e3>
kk4= 62 <0,1e3>
ll4= 6 <0,1e3>
mm4= 1 <0,1e3>
nn4= 1000 <0,1e4>
oo4= 60 <0,1e3>
pp4= 20 <0,1e3>
qq4= 60 <0,1e3>
rr4= 8 <0,1e3>
ss4= 50 <0,1e3>
zss4 = 0.9 <0,1e3> : steady state inactivation of glt
}
STATE {
w4 z4
}
ASSIGNED {
ik (mA/cm2)
gkcnab2 (mho/cm2)
winf4 zinf4
wtau4 (ms) ztau4 (ms)
}
LOCAL wexp4, zexp4
BREAKPOINT {
SOLVE states
gkcnab2 = gkcnab2bar*(w4^4)*z4
ik = gkcnab2*(v - ek)
}
UNITSOFF
INITIAL {
trates(v)
w4 = winf4
z4 = zinf4
}
PROCEDURE states() { :Computes state variables m, h, and n
trates(v) : at the current v and dt.
w4 = w4 + wexp4*(winf4-w4)
z4 = z4 + zexp4*(zinf4-z4)
VERBATIM
return 0;
ENDVERBATIM
}
LOCAL q10
PROCEDURE rates(v) { :Computes rate and other constants at current v.
:Call once from HOC to initialize inf at resting v.
winf4 = (1 / (1 + exp(-(v + aa4) / bb4)))^cc4
zinf4 = zss4 + ((1-zss4) / (1 + exp((v + dd4) / ee4)))
wtau4 = (ff4 / (gg4*exp((v+hh4) / ii4) + jj4*exp(-(v+kk4) / ll4))) + mm4
ztau4 = (nn4 / (exp((v+oo4) / pp4) + exp(-(v+qq4) / rr4))) + ss4
}
PROCEDURE trates(v) { :Computes rate and other constants at current v.
:Call once from HOC to initialize inf at resting v.
LOCAL tinc
TABLE winf4, wexp4, zinf4, zexp4
DEPEND dt, celsius FROM -150 TO 150 WITH 300
q10 = 3^((celsius - 20)/10)
rates(v)
tinc = -dt * q10
wexp4 = 1 - exp(tinc/wtau4)
zexp4 = 1 - exp(tinc/ztau4)
}
UNITSON