Inject = 0
back=0
//////// load needed templates////////////
if(!back){ load_file("nrngui.hoc") }
if(!back){ load_file("template/ObliquePath.hoc") }
if(!back){ load_file("template/BasalPath.hoc" ) }
objref econ,f1,f2,f3,ss,cvode
f1= new File()
f2= new File()
f3= new File()
ss=new SaveState()
cvode = new CVode(1)
x=cvode.active(1)
restart=0
v_init=-70
strdef morphology_location, morpho_path, ObliqueTrunkSection, BasalTrunkSection
objref vRP, vAPEX
proc xopen_morphology(){
sprint(morpho_path,"%s/%s",morphology_location,$s1)
xopen(morpho_path)
}
// Carmen
morphology_location = "pc2b"
ObliqueTrunkSection = "trunk[17]"
BasalTrunkSection = "trunk[7]"
xopen_morphology("cell.hoc") //reads morpholofy from the file
xopen_morphology("cell-analysis-simple.hoc") //reads simplified version of CA
xopen("lib/vector-distance.hoc")
//-----------------------------------------------------------------------------------------------------
printf("Setting up cell\n") // load cell-setup to
xopen("cell_setup_pc2b.hoc") // specify all mechanisms, membrane properties etc
///////////////////Load File//////////////////////
//if(!back) load_file("kca.ses")
if(!back) { load_file("basic.ses") }
/*cvode_active(1)*/
////// Spike counter//////////
objref apc, v1
proc insert_APC() {
apc = new APCount(0.5)
apc.thresh = $1
v1 = new Vector()
apc.record(v1)
}
proc init() {
if(restart){
f1.ropen("state.old")
ss.fread(f1)
f1.close
finitialize(v_init)
ss.restore()
t=0
fcurrent()
cvode.re_init()
} else {
finitialize(v_init)
fcurrent()
}
}
////////////////////Main///////////////////
nsyn=10
objref s[nsyn], rsyn[nsyn], nc[nsyn]
objref rsynmda[nsyn], ncnmda[nsyn]
objref rect,recv,reci,savv,savi,savt
objref TimeVector,AmplitudeVector,MyCurrentClamp
proc main(){
recv =new Vector()
rect =new Vector()
reci =new Vector()
//tol=cvode.atolscale(&soma.v(0.5),1e-5)
period=95.75
phase0=67.8234
dur=3
amp2=0.0
tstop=16000
in=0
th=-14
access soma
insert_APC(th)
f3.wopen("spike.dat")
current=0.0 // 0.18
del=50
StartTime=10000
CurrentBase=0.0
PulseAmplitude=-0.2
PulseDuration=500
if (Inject) {
//trunk[16] MyCurrentClamp=new CurrentClamp(0.3)
} else {
soma MyCurrentClamp=new CurrentClamp(0.5)
}
MyCurrentClamp.Delay=0
MyCurrentClamp.Duration=1e9
TimeVector=new Vector(3)
TimeVector.x[0]=0
TimeVector.x[1]=StartTime
TimeVector.x[2]=StartTime+PulseDuration
AmplitudeVector=new Vector(3)
AmplitudeVector.x[0]=CurrentBase
AmplitudeVector.x[1]=CurrentBase+PulseAmplitude
AmplitudeVector.x[2]=CurrentBase
AmplitudeVector.play(&MyCurrentClamp.Amplitude,TimeVector)
recv.record(&v(0.5))
rect.record(&t)
reci.record(&MyCurrentClamp.i)
//////////////run////////////////////////
run()
/////////////////////////////////////
savv= new File()
savt= new File()
savi = new File()
savv.wopen("v.txt")
savt.wopen("time.txt")
savi.wopen("i.txt")
recv.printf(savv)
rect.printf(savt)
reci.printf(savi)
savv.close
savt.close
savi.close
// print the spike number
if(!back) printf("\n Current: %.4f nA | # Spikes: %d\n", current, apc.n)
if(!back) printf("___________________\n")
v1.printf(f3)
f3.close
f2.wopen("state.new")
ss.save
ss.fwrite(f2)
f2.close
}
main()
/******** end file ******/