// ADD VGAT+ SYNAPSES TO MORPHOLOGY.
// Preallocate objects for inhibition.
totVgatAt = 100000
objref nsVgatAt[totVgatAt],ncVgatAt[totVgatAt],synVgatAt[totVgatAt]
// This function takes a SectionRef and a vector with surface area synapse
// scaling and places the number of corresponding Vgat+ inhibitory synapses.
// Note that the low index of the first placed synapse needs to be specified
// in order to give indices to all of the generated synapse.
//
// $o1: SectionRef instance. Contains the section to implement with the scaling
// rule.
// $o2: Vector instance. Contains the scaling rule to implement. If the
// density is uniform across the branch (e.g., 0.1 synapses/um2), then
// simply supply as
// foo = new Vector(1); foo.x[0] = 0.1; $o1 = foo
// Conversely, if
// there is spatial variation across the branch (say, 0.1 synapses/um2 in the
// first third, 0.2 synapses/um2 in the second third, and 0.3 synapses/um2
// in the final third), then, supply as
// foo = new Vector(3) ; foo.x[0] = 0.1, foo.x[1] = 0.2;
// foo.x[2] = 0.3 ; $o1 = foo
// $3: numeric. The index of the first synapse to be created and placed.
//
// The number of synapses added is returned.
func addVgatInhibition() {local nDiv,runningSa,synAdd,synCur,ii,jj
nDiv = $o2.size() // number of divisions for the compartment
synCur = $3
// Place synapses.
$o1.sec {
for ii=1,nDiv{
runningSa = 0
synAdd=0
for(x,0){
if(((ii-1)/nDiv)<x_eff(x)){
if(x_eff(x)<(ii/nDiv+0.00000001)){
runningSa += area(x)
if(int(runningSa*($o2.x[ii-1]))>(synAdd+0.00001)){
toAdd = int(runningSa*($o2.x[ii-1])-synAdd)
for jj=1,toAdd{
synCur = synCur + 1
synAdd = synAdd + 1
//print "\t",secname(),"\t",x,"\t",synCur-1
synVgatAt[synCur-1] = new inhSyn(x)
synVgatAt[synCur-1].vgat = 1
synVgatAt[synCur-1].xEff = x_eff(x)
nsVgatAt[synCur-1] = new NetStim(x)
ncVgatAt[synCur-1] = new NetCon(nsVgatAt[synCur-1],synVgatAt[synCur-1])
}
}else{
toAdd = 0
}
vgatAt_syns(x) = toAdd
}
}
}
}
}
return synCur-$3
}
synInd = 0 // first index to be used
objref curSec // rolling SectionRef to currently accessed section
// Define tuft densities and add tuft synapses.
objref denVgatTuftTerm,denVgatTuftPar
denVgatTuftTerm = new Vector(3)
denVgatTuftTerm.x[0] = 0.09//0.075
denVgatTuftTerm.x[1] = 0.11//0.085
denVgatTuftTerm.x[2] = 0.135//0.1275
denVgatTuftPar = new Vector(3)
denVgatTuftPar.x[0] = 0.105
denVgatTuftPar.x[1] = 0.105
denVgatTuftPar.x[2] = 0.1
nVgatTuft = 0
forsec tuftList {
curSec = new SectionRef()
if(isTerm_id){
numAdded = addVgatInhibition(curSec,denVgatTuftTerm,synInd)
}else{
numAdded = addVgatInhibition(curSec,denVgatTuftPar,synInd)
}
synInd+=numAdded
nVgatTuft+=numAdded
}
// print "The total number of VGAT+ synapses in tuft is: ",nVgatTuft
// Define oblique densities and add oblique synapses.
objref denVgatObl
denVgatObl = new Vector(3)
denVgatObl.x[0] = 0.04
denVgatObl.x[1] = 0.04
denVgatObl.x[2] = 0.04
nVgatObl = 0
forsec obliqueList {
curSec = new SectionRef()
numAdded = addVgatInhibition(curSec,denVgatObl,synInd)
synInd+=numAdded
nVgatObl+=numAdded
}
// print "The total number of VGAT+ synapses in obliques is: ",nVgatObl
// Define apical trunk densities and add synapses.
// Note this includes a distance-dependent scaling factor.
objref denVgatPrim,denVgatPrimTemp
denVgatPrim = new Vector(1)
denVgatPrim.x[0] = 0.056
denVgatPrimTemp = new Vector(denVgatPrim.size()) // a rolling density with distant-dependent scaling
nVgatPrim = 0
soma.sec{distance()}
forsec primList {
curSec = new SectionRef()
theDist = distance(0.5)
scaleFact = 1-theDist/225
if(scaleFact<0){scaleFact=0}
denVgatPrimTemp.copy(denVgatPrim)
denVgatPrimTemp.mul(scaleFact)
numAdded = addVgatInhibition(curSec,denVgatPrimTemp,synInd)
synInd+=numAdded
nVgatPrim+=numAdded
}
// print "The total number of VGAT+ synapses in main apical is: ",nVgatPrim
// Add synapses to soma.
objref denVgatSoma
denVgatSoma = new Vector(1)
denVgatSoma.x[0] = 0 // 0.064 is correct, but don't want somatic inhibition
nVgatSoma = 0
soma.sec {
curSec = new SectionRef()
numAdded = addVgatInhibition(curSec,denVgatSoma,synInd)
synInd+=numAdded
nVgatSoma+=numAdded
}
// print "The total number of VGAT+ synapses in soma is: ",nVgatSoma
// Add synapses to basals.
objref denVgatBasalPrim,denVgatBasalSec,denVgatBasalTerm
denVgatBasalPrim = new Vector(1)
denVgatBasalPrim.x[0] = 0.09
denVgatBasalSec = new Vector(1)
denVgatBasalSec.x[0] = 0.08
denVgatBasalTerm = new Vector(3)
denVgatBasalTerm.x[0] = 0.05
denVgatBasalTerm.x[1] = 0.05
denVgatBasalTerm.x[2] = 0.03
nVgatBasal=0
forsec basalList {
curSec = new SectionRef()
if(isTerm_id){
numAdded = addVgatInhibition(curSec,denVgatBasalTerm,synInd)
}else{
if(abs(brOrd_id-1)<0.001){
numAdded = addVgatInhibition(curSec,denVgatBasalPrim,synInd)
}else{
if(abs(isTerm_id)){
numAdded = addVgatInhibition(curSec,denVgatBasalTerm,synInd)
}else{
numAdded = addVgatInhibition(curSec,denVgatBasalSec,synInd) // keep as 2ary for time being
}
}
}
synInd+=numAdded
nVgatBasal+=numAdded
}
// print "The total number of VGAT+ synapses in basals is: ",nVgatBasal
// Note the number of synapses that were created.
// print "The total number of VGAT+ synapses is: ",synInd
totVgatAt = synInd-1