//where am I?
//{system("pwd")}
// debug flags, debugging NEURON is fun!
verb = 0
runverb = 0
snv_verbose = 0
spinevrb = 0
ncverb = 0
ssverb = 0
// program flags
activepreinit = 1 // put in all those fancy ion channels?
ssinitenabled = 1 // run the steady-state init stuff?
fakerun = 0 // don't really do a run, end run immediately, used to read out spike rasters if needed later
spinesfl = 0 // spines in use?
poisson=1 // poisson stimulus train or some other predetermined spike train
synrec=0 // record synaptic conductance/current
// load dependencies
{load_file("stdgui.hoc")}
{load_file("global.hoc")} // misc functions
{load_file("ssinit.hoc")} // steady-state init and 'run' control
{load_file("insertmech.hoc")} // used by preinit() function to plug in instrinsic membrane properties
{load_file("synsuper.hoc")} // used by preinit() function to plug in synapses
{load_file("netconsynstimbundle.hoc")} // used by preinit() function to deliver presynaptic netcon events
{load_file("basal_project_params.hoc")} // parameters
//synapse placeholder list
objref synphlist
synphlist = new List("synph")
//super synapse list
objref ssynlist
ssynlist = new List("synsuper")
objref ampalist
objref nmdalist
objref ampaglist
objref nmdaglist
objref ampailist
objref nmdailist
objref nmdablist
ampaglist = new List()
nmdaglist = new List()
ampailist = new List()
nmdailist = new List()
nmdablist = new List()
ampalist = new List("dsyn")
nmdalist = new List("NMDA_dsyn")
// load morphology
{load_file("morpho/newj4line.hoc")} // smoothed j4
{load_file("morpho/j4-sets.hoc")} // all those j4 lists
{load_file("morpho/j4_axon.hoc")} // give j4 an axon
soma_con_pt = 1
access soma
soma distance(0,soma_con_pt)
proc refreshnseg() {local nsegnew localobj active_seclist, pasactive_seclist
pasactive_seclist = new SectionList()
active_seclist = $o1
forsec myelin_list active_seclist.append()
forsec soma_list active_seclist.append()
forsec axon_list active_seclist.append()
forsec active_seclist {
nsegnew = int(L/seg_per_char_l/2)*2+1 //at least 1 segment every seg_per_char_l (10) um
if (nsegnew>nseg) nseg = nsegnew //only increase nseg
nsegnew = int((L/(d_lambda*lambda_f(lambdaf))+(1-d_lambda))/2)*2+1 //dlambda rule recommended by Hines/Carnevale
if (nsegnew>nseg) nseg = nsegnew
nsegnew = 3 //make nseg at least 3, to please prerun() histogram function
if (nsegnew>nseg) nseg = nsegnew
}
forall pasactive_seclist.append()
pasactive_seclist.remove(active_seclist)
forsec pasactive_seclist nseg=1 // make the nonactive (not directly excited subtree[s]) sections have fewer segments
soma distance(0,soma_con_pt)
}
objref r, rin
obfunc newstimvec() {localobj indvec, r, syn
r = $o1
indvec = new Vector(tsamp)
indvec.setrand(r).integral().where("<=",tstop)
if (numarg()>1) {
syn = $o2
if (syn.e1flag) indvec.where(">=",syn.e1del)
if (syn.e2flag) indvec.where("<=",syn.e2del)
}
return indvec
}
objref ex_stim_vecs, snv_synList, fihs
ex_stim_vecs = new List()
snv_synList = new List()
fihs = new List()
proc place_glu_freq() {\
local loc, synidx, ampagmax, nmdagmax, pulse1, pulse2, mechloc, nsyn\
localobj ex_stim_vec, snv_newSyn, tmp_stim_vec, syn, rand, execcmd, nil, ncssb, theseedvec
execcmd = new String()
synidx = $1
loc = $2
ampagmax = $3
nmdagmax = $4
rand = $o5.rand
syn = $o5.syn
nsyn = $6
mechloc = loc // if channels directly on dendrite
if (spinesfl) mechloc = 1.0 // if we have spines
pulse1=2
pulse2=22
if (poisson) {
ex_stim_vec = newstimvec(rand,syn)
for i=2,nsyn {
tmp_stim_vec = newstimvec(rand,syn)
ex_stim_vec.append(tmp_stim_vec)
}
ex_stim_vecs.append(ex_stim_vec)
} else {
ex_stim_vec = new Vector()
if (syn.e1flag) ex_stim_vec.append(syn.e1del)
if (syn.e2flag) ex_stim_vec.append(syn.e2del)
ex_stim_vecs.append(ex_stim_vec)
if (snv_verbose) ex_stim_vec.printf
ampagmax *= nsyn
nmdagmax *= nsyn
}
if (spinesfl) {
if (spinevrb) print "creating spine"
{sprint(execcmd.s, "create spinehead%d, spineneck%d", synidx, synidx) execute(execcmd.s)}
{sprint(execcmd.s, "spineneck%d connect spinehead%d(0), 1", synidx, synidx) execute(execcmd.s)}
{sprint(execcmd.s, "connect spineneck%d(0), %g", synidx, loc) execute(execcmd.s)}
{sprint(execcmd.s, "push_section(\"spineneck%d\")", synidx) execute(execcmd.s)}
spine_list.append()
{Ra = global_Ra L = spine_neck_L diam = spine_neck_diam}
pop_section()
{sprint(execcmd.s, "push_section(\"spinehead%d\")", synidx) execute(execcmd.s)}
spine_list.append()
{Ra = global_Ra L = spine_head_L diam = spine_head_diam}
if (spinevrb) print "created spine"
}
// AMPA component
snv_newSyn = new dsyn(mechloc)
snv_newSyn.tau1 = dsyntau1
snv_newSyn.tau2 = dsyntau2
snv_newSyn.gmax = ampagmax
//netcon = new NetCon(source, target, threshold, delay, weight)
ncssb = new netconsynstimbundle(new NetCon(nil,snv_newSyn,1,fixed_step_dt*10,1),snv_newSyn,ex_stim_vec)
fihs.append(new FInitializeHandler(2,"doevents()",ncssb))
snv_synList.append(ncssb)
// NMDA component
snv_newSyn = new NMDA_dsyn(mechloc)
snv_newSyn.tau1 = nmdadsyntau1
snv_newSyn.tau2 = nmdadsyntau2
snv_newSyn.gmax = nmdagmax
snv_synList.append(snv_newSyn)
//netcon = new NetCon(source, target, threshold, delay, weight)
ncssb = new netconsynstimbundle(new NetCon(nil,snv_newSyn,1,fixed_step_dt*10,1),snv_newSyn,ex_stim_vec)
fihs.append(new FInitializeHandler(2,"doevents()",ncssb))
snv_synList.append(ncssb)
if (spinesfl) pop_section()
if (snv_verbose) print "syn ", synidx, " - Glu @ ", secname(), ": ", loc, "ampa GMAX=", ampagmax, ", nmda GMAX=", nmdagmax
}
proc prerun() {local i, loc localobj sl2
tsamp = tstop/dt+1
ex_stim_vecs.remove_all()
fihs.remove_all()
snv_synList.remove_all()
forsec spine_list delete_section()
spine_list.remove(spine_list)
if (spinesfl) {
runsyn(0)
} else {
if (poisson) {
runsyn(0)
} else {
runsyn(1)
}
}
}
proc runsyn() {local nsyn, i, j, k, loc, tloc, maxspan, nsegrun\
localobj locs, locsnsyn, tlocv, nseglocs, sl2, syn, thelist, gvec
thelist = ssynlist
nsegrun = $1
if (runverb) print "nsegrun: ", nsegrun
k=0
if (runverb) print thelist.count()
for i= 0,thelist.count()-1 {
syn = thelist.o(i).syn
locs = new Vector()
loc = syn.get_loc()
//print i, syn.sloc, syn.nsyn, loc, secname()
if(-1!=syn.sloc) loc = (syn.sloc-distance(0))/L
nsyn = syn.nsyn
if (nsyn) {
if (nsyn==1) locs = new Vector(1,loc)
if (nsyn==2) {
maxspan = spnspc/L/2
locs.append(loc-maxspan,loc+maxspan)
}
if (nsyn>=3) {
maxspan = spnspc*((nsyn-1)/2)/L
locs.indgen(loc-maxspan,loc+maxspan,spnspc/L-float_epsilon)
}
if (runverb) print maxspan, loc, L " locs vector built"
if (runverb) locs.printf
locsnsyn = new Vector(locs.size(),1)
if (nsegrun) {
locsnsyn = locs.histogram(0,1,1/(nseg-float_epsilon))
locs = new Vector(locsnsyn.size())
locs.indgen().add(-1).mul(1/nseg).add(1/nseg/2)
}
if (runverb) locs.printf
if (runverb) locsnsyn.printf
if (runverb) print locsnsyn.sum, nsyn
for j=0,locs.size()-1{
if (locsnsyn.x[j]) {
place_glu_freq(k,locs.x[j],snv_ampa_gmax*syn.noampablock,snv_ampa_gmax*syn.ratio*syn.nonmdablock,thelist.o(i),locsnsyn.x[j])
k+=1
}
}
}
pop_section()
}
if (synrec) {
ampaglist.remove_all()
nmdaglist.remove_all()
ampailist.remove_all()
nmdailist.remove_all()
nmdablist.remove_all()
for i = 0,ampalist.count()-1 {
gvec = new Vector()
gvec.record(&alist.o(i).g)
ampaglist.append(gvec)
gvec = new Vector()
gvec.record(&alist.o(i).i)
ampailist.append(gvec)
}
for i = 0,nmdalist.count()-1 {
gvec = new Vector()
gvec.record(&nmdalist.o(i).g)
nmdaglist.append(gvec)
gvec = new Vector()
gvec.record(&nmdalist.o(i).i)
nmdailist.append(gvec)
gvec = new Vector()
gvec.record(&nmdalist.o(i).mgB)
nmdablist.append(gvec)
}
}
}
proc preinit() {
//mostly passive stuff
forall {
insertmech(pas_suffix.s,global_epas,"e")
insertmech(pas_suffix.s,1/global_Rm,"g")
Ra = global_Ra
cm= somatic_Cm
}
// exceptions along the axon
forsec node_list {
insertmech(pas_suffix.s,1/node_Rm,"g")
}
forsec myelin_list {
cm = myelin_Cm
}
// spine 'correction'
forsec basal_leaves {
insertmech(pas_suffix.s,1/dendritic_Rm,"g")
cm= dendritic_Cm
}
//mostly active stuff
if (activepreinit) {
forall{
// no universals atm
}
forsec dendrite_list {
insertmech(hhk_suffix.s,taun_gk,"taun")
insertmech(hhna_suffix.s,taum_gna,"taum")
insertmech(hhna_suffix.s,tauh_gna,"tauh")
insertmech(hhna_suffix.s,tausb_gna,"tausb")
insertmech(hhna_suffix.s,taus_gna,"taus")
insertmech(hhna_suffix.s,tausvh_gna,"tausvh")
insertmech(hhna_suffix.s,tausvs_gna,"tausvs")
insertmech(hhk_suffix.s,pown_gk,"pown")
insertmech(hhna_suffix.s,powm_gna,"powm")
insertmech(hhna_suffix.s,powh_gna,"powh")
insertmech(hhna_suffix.s,pows_gna,"pows")
insertmech(hhna_suffix.s,dend_gna,"gbar",dend_gna_slope)
insertmech(hhk_suffix.s,dend_gk,"gbar",dend_gk_slope)
}
forsec spine_list {
// passive spines atm
}
forsec soma_list {
insertmech(hhk_suffix.s,taun_gk,"taun")
insertmech(hhna_suffix.s,taum_gna,"taum")
insertmech(hhna_suffix.s,tauh_gna,"tauh")
insertmech(hhna_suffix.s,tausb_gna,"tausb")
insertmech(hhna_suffix.s,taus_gna,"taus")
insertmech(hhna_suffix.s,tausvh_gna,"tausvh")
insertmech(hhna_suffix.s,tausvs_gna,"tausvs")
insertmech(hhk_suffix.s,pown_gk,"pown")
insertmech(hhna_suffix.s,powm_gna,"powm")
insertmech(hhna_suffix.s,powh_gna,"powh")
insertmech(hhna_suffix.s,pows_gna,"pows")
insertmech(hhna_suffix.s,soma_gna)
insertmech(hhk_suffix.s,soma_gk)
}
forsec node_list {
insertmech(hhk_suffix.s,taun_gk,"taun")
insertmech(hhna_suffix.s,taum_gna,"taum")
insertmech(hhna_suffix.s,tauh_gna,"tauh")
insertmech(hhna_suffix.s,tausb_gna,"tausb")
insertmech(hhna_suffix.s,taus_gna,"taus")
insertmech(hhna_suffix.s,tausvh_gna,"tausvh")
insertmech(hhna_suffix.s,tausvs_gna,"tausvs")
insertmech(hhk_suffix.s,pown_gk,"pown")
insertmech(hhna_suffix.s,powm_gna,"powm")
insertmech(hhna_suffix.s,powh_gna,"powh")
insertmech(hhna_suffix.s,pows_gna,"pows")
insertmech(hhna_suffix.s,axon_gna)
insertmech(hhk_suffix.s,axon_gk)
}
forsec myelin_list {
insertmech(hhna_suffix.s,taum_gna,"taum")
insertmech(hhna_suffix.s,tauh_gna,"tauh")
insertmech(hhna_suffix.s,tausb_gna,"tausb")
insertmech(hhna_suffix.s,taus_gna,"taus")
insertmech(hhna_suffix.s,tausvh_gna,"tausvh")
insertmech(hhna_suffix.s,tausvs_gna,"tausvs")
insertmech(hhna_suffix.s,powm_gna,"powm")
insertmech(hhna_suffix.s,powh_gna,"powh")
insertmech(hhna_suffix.s,pows_gna,"pows")
insertmech(hhna_suffix.s,dend_gna)
}
forsec axon_list { //axon_hillock and axon_is
insertmech(hhk_suffix.s,taun_gk,"taun")
insertmech(hhna_suffix.s,taum_gna,"taum")
insertmech(hhna_suffix.s,tauh_gna,"tauh")
insertmech(hhna_suffix.s,tausb_gna,"tausb")
insertmech(hhna_suffix.s,taus_gna,"taus")
insertmech(hhna_suffix.s,tausvh_gna,"tausvh")
insertmech(hhna_suffix.s,tausvs_gna,"tausvs")
insertmech(hhk_suffix.s,pown_gk,"pown")
insertmech(hhna_suffix.s,powm_gna,"powm")
insertmech(hhna_suffix.s,powh_gna,"powh")
insertmech(hhna_suffix.s,pows_gna,"pows")
insertmech(hhna_suffix.s,axon_gna)
insertmech(hhk_suffix.s,axon_gk)
}
}
forall { //set batteries
if(ismembrane("k_ion")) ek = global_ek
if(ismembrane("na_ion")) ena = global_ena
}
}