This simulation was used in the following article: Neymotin SA, Hilscher MM, Moulin TC, Skolnick Y, Lazarewicz MT, Lytton WW. (2013) Ih Tunes Theta/Gamma Oscillations and Cross-Frequency Coupling In an In Silico CA3 Model. PLoS ONE 8(10):e76285. doi:10.1371/journal.pone.0076285 Complete article PDF is available here: http://neurosimlab.org/pdfs/poneIh13.pdf For questions email: samn at neurosim dot downstate dot edu This simulation was tested/developed on LINUX systems, but may run on Microsoft Windows or Mac OS. To run, you will need the NEURON simulator (available at http://www.neuron.yale.edu) compiled with python enabled. To draw the output you will need to have Matplotlib installed ( http://matplotlib.org/ ). Instructions: Unzip the contents of the zip file to a new directory. compile the mod files from the command line with: nrnivmodl *.mod The nrnivmodl command will produce an architecture-dependent folder with a script called special. On 64 bit systems the folder is x86_64. To run the simulation from the command line use: nrniv -python sim.py [simulation duration in milliseconds] (note that the last argument is optional, default simulation duration is 1000.0 ms) This will start NEURON with the python interpreter and load the mechanisms and simulation. Next, the network and inputs will be setup. Then the simulation will be run for 1 second of simulation time, varying the level of Ih conductance (5 levels) in both pyramidal and basket cells simultaneously. These simulations will take a few minutes to run (depending on your CPU) and several GB of RAM. Once the simulations finish running, the simulated local field potentials (LFPs) will be calculated, along with their power spectral density (PSD). The optional last argument on the command line specifies the duration of tnhe simulation in milliseconds. It should be <= 8000.0 (for 8 seconds of simulation time). Since many users do not have availability of high-performance computers, the default simulation duration is 1000.0 ms. Simulations in the paper were run for 8000.0 ms, so if you have a high-performance computer available, you can set the duration to 8000.0 in the command line above. When simulations are run for 8000.0 ms, the PSD is calculated and peak theta,gamma are displayed. When simulations are run for 1000.0 ms, the theta and gamma peaks are not calculated, since there is not sufficient time to extract an accurate response. Note that since Matlab is not assumed to be available, the results will appear slightly different from the paper (Matlab's pmtm function was used to get the power spectra in the paper). Once PSD is calculated, the peak theta and gamma power for each simulation will be extracted. Finally, output will be drawn using matplotlib (similar to figure 10 of the paper). The top panel will show the LFPs from the simulations arranged from low Ih to high Ih (going up). These LFPs are also color coded. The bottom two panels will display the peak theta,gamma power and frequency with each dot corresponding to the color code in the top panel (displayed when running simulation for 8000.0 ms; see above). Note that since only a single simulation is run here there will be some differences from the figure in the paper, which consisted of multiple simulations. However, the overall trend is the same. 20140619 Model updated by Sam Neymotin to supply new versions of misc.h, misc.mod, stats.mod, vecst.mod which fixes type checking errors that some compilers generate as reported by a kind modeler. Also the readme was made narrower for nicer display in modeldb. 20160915 This updated version from the Lytton lab allows their models which contain misc.mod and misc.h to compile on the mac. 20220523 Updated MOD files to contain valid C++ and be compatible with the upcoming versions 8.2 and 9.0 of NEURON. 20221216 Python3 migration via 2to3.