// proc MechList( List ) - adds String with the name of all membrane mechanisms in cas to List
// dependency: none
// proc getglist( List ) - get the names of all membrane mechanisms in cas with a 'g' variable
// dependency: MechList (local)
// func Rm( channel name(s), Rm/Gm flag ) - returns membrane resistance or conductance
// dependency: MakeStringList (local), getglist (local)
// func meanRm( sections, mechanism(s), Rm/Gm flag)
// dependency: Rm (local), MakeStringList (local), getglist (local)
// proc HCNDist( strdef gradient name ) - set the gradient of HCN distributions
// dependency: issplit, StopPar, StartPar (parinit); meanRm (local)
load_file("chanAnalysis.hoc")
proc MechList() { local i, cas localobj ml, mt, strobj
//MechList( string list, cas flag )
// creates a list of strings with the names of all membrane mechanisms in cas or all sections
if (object_id($o1,1) == -1) $o1 = new List() // if object is null make a new List
ml = $o1
mt = new MechanismType(0)
if (numarg()>1) cas = $2 else cas = 0
for i=0,mt.count()-1 {
strobj = new String()
mt.select(i)
mt.selected(strobj.s)
if (cas) {
if (MechChk(strobj.s) == 1) ml.append(strobj)
} else ml.append(strobj)
}
}
proc PPList() { local i localobj ml, mt, strobj
//PPList( string list, type )
// creates a list of strings with the names of point processes in cas
if (object_id($o1,1) == -1) $o1 = new List() // if object is null make a new List
ml = $o1
mt = new MechanismType(1)
// if (numarg()>1) cas = $2 else cas = 0
strobj = new String()
for i=0,mt.count()-1 {
mt.select(i)
mt.selected(strobj.s)
if (MechChk(strobj.s) == 1) ml.append(strobj)
}
}
func MechChk() { local i,val localobj ms, strobj
//bool = MechChk( string )
// checks whether membrane mechanism named "string" is in current section. Returns 1 if yes, 0 if not
ms = new MechanismStandard($s1,0)
val = -1.314e20 // assuming no parameter will be equal to this value
strobj = new String()
if (ms.count() > 0) {
ms.name(strobj.s,0)
ms.set(strobj.s,val)
ms.in()
i = (val != ms.get(strobj.s))
}
return i
}
proc getglist() { local i,j,flag, cas localobj glist, ms, SF
// getglist( List, cas flag )
// generate a list of conductance mechanisms in cas or all sections (all membrane mechs with a 'g' value)
if (numarg()>1) cas = $2 else cas = 0
if (object_id($o1,1) == -1) $o1 = new List() // if object is null make a new List
glist = $o1
MechList(glist, cas)
SF = new StringFunctions()
for (i=glist.count()-1; i>=0; i=i-1) {
ms = new MechanismStandard(glist.o(i).s, 0)
flag=0
for j=0,ms.count()-1 {
ms.name(strtmp,j)
if (SF.substr(strtmp,"g_") != -1) {
flag=1
}
}
if (flag == 0) glist.remove(i)
}
}
proc getgvec() { local i,j localobj gvec, ml, ms, SF
// getgvec( Vector, List )
// getgvec( Vector )
// generate vector of conductance values of all mechanisms in List. If no List given, calls
// MechList and uses all conductance mechanisms
gvec = $o1
if (numarg()>1) {
ml = $o2
} else {
ml = new List()
MechList(ml)
}
if (object_id(gvec,1) == -1) gvec=new Vector(ml.count(),0)
SF = new StringFunctions()
for i=0,ml.count()-1 {
ms = new MechanismStandard(ml.o(i).s, 0)
for j=0,ms.count()-1 {
ms.name(strtmp,j)
if (SF.substr(strtmp,"g_") != -1) {
gvec.x[i]=1
}
}
}
}
proc MakePassive() {local i,Gsum,argn,vi localobj sl, gls
argn = numarg()
vi = v_init
if (verbosity > 3) for i=1,argn print argtype(i)
if (argn > 1) {
vi = $2
} else if (argn > 0) {
if (argtype(1)==2) {
sl = new SectionList()
forall ifsec $s1 sl.append()
if (verbosity > 4) sl.printnames()
} else if (argtype(1)==1) sl = $o1
} else {
sl = new SectionList()
sl.append()
}
init(v_init)
forsec sl {
gls = new List()
getglist(gls,1)
for (x,0) {
RmGtot=0
for i=0,gls.count()-1 {
if (ismembrane(gls.o(i).s)) {
sprint( strtmp, "RmGtot += g_%s", gls.o(i).s)
execute(strtmp)
sprint( strtmp, "uninsert %s", gls.o(i).s)
execute(strtmp)
}
}
insert pas
g_pas = RmGtot
e_pas = vi
}
}
}
func Rm() { local i,flag localobj gls
// val = Rm( strdef channel name )
// val = Rm( List channel name(s) )
// val = Rm( channel name(s), Rm/Gm flag )
// val = Rm( 0, Rm/Gm flag )
// measure membrane resistivity megaohm*cm^2 (flag == 0) or conductivity S/cm^2 (flag == 1)
flag = 0
RmGtot=0
if (numarg() > 0) {
if (argtype(1) == 1) {
gls = $o1
} else if (argtype(1)==2) {
gls = new List()
MakeStringList(gls,$s1)
} else if (argtype(1)==0) {
gls = new List()
getglist(gls)
}
} else {
gls = new List()
getglist(gls)
}
if (numarg() > 1) flag = $2
for i=0,gls.count()-1 {
if (ismembrane(gls.o(i).s)) {
sprint( strtmp, "RmGtot += g_%s", gls.o(i).s)
//if (verbosity > 3) printf("%s", strtmp)
execute(strtmp)
}
}
if (flag ==1) {
return RmGtot
} else {
if (RmGtot == 0) return 1e30 else return 1e-3/RmGtot
}
}
func sum_state() { local err
// sum = sum_state( SectionList, strdef )
Ssum=0
err=-1
sprint( strtmp, "Ssum += %s*area(0.5)", $s2)
forsec $o1 {
if (err==-1) {
err = execute1(strtmp)
if (err==0) return -1
} else execute(strtmp)
}
return Ssum
}
func meanRm() { local argn,i,flag, Asum,Gsum localobj gls, sl
// val = meanRm( sections, mechanism(s), Rm/Gm flag)
// 'sections' can be a SectionList or string specifying sections to measure
// 'mechanisms' can be a List of membrane mechanisms, a string with name of mechanism, or
// else a list of all membrane mechanisms is generated
// if Rm/Gm flag == 1 returms mean membrane conductivity (S/cm^2).
// measure membrane resistivity megaohm*cm^2 (flag == 0) or conductivity S/cm^2 (flag == 1)
flag = 0
Gsum=0
Asum = 0
argn = numarg()
if (verbosity > 3) for i=1,argn print argtype(i)
if (argn >= 1) {
if (argtype(1)==2) {
sl = new SectionList()
forall ifsec $s1 sl.append()
if (verbosity > 4) sl.printnames()
} else if (argtype(1)==1) sl = $o1
} else {
if (verbosity > 1) printf("Must give at least 1 input arg: meanRm\n")
return -1
}
if (argn >= 2) {
if (argtype(2)==1) {
gls = $o2
} else if (argtype(2)==2) {
gls = new List()
MakeStringList(gls,$s2)
} else if (argtype(2)==0) {
gls = new List()
getglist(gls)
}
} else {
gls = new List()
getglist(gls)
}
if ((argn >= 3) && (argtype(3)==0) ) flag = $3
if ( SectionListCount(sl) == 0 ) return -1
if (object_id(sl) != 0) {
forsec sl for (x,0) {
Asum += area(x) // µm2
Gsum += Rm(gls,1)*area(x) // S/cm2*µm2 = S*10^-8
}
} else {
forall for (x,0) {
Asum += area(x) // µm2
Gsum += Rm(gls,1)*area(x) // S/cm2*µm2 = S*10^-8
}
}
if (flag ==1) {
return Gsum/Asum // S/cm2
} else {
if (Gsum == 0) return 1e30 else return Asum*1e-3/Gsum // MΩ•cm2
}
}
obfunc getchan() { local i,flag localobj glist, ms, SF
// mech = getchan( strdef )
// generate a list of conductance mechanisms in section (all membrane mechs with a 'g' value)
glist = new List() // if object is null make a new List
MechList(glist)
SF = new StringFunctions()
for (i=glist.count()-1; i>=0; i=i-1) {
if (SF.substr( glist.o(i).s, $s1) != -1) {
ms = new MechanismStandard( glist.o(i).s, 0)
flag=1
}
}
return ms
}
func meanCaI() { local argn,i, A,ci localobj sl
//meanCaI( sections )
ci= 0
A = 0
argn = numarg()
if (verbosity > 3) for i=1,argn print argtype(i)
if (argn >= 1) {
if (argtype(1)==2) {
sl = new SectionList()
forall ifsec $s1 sl.append()
if (verbosity > 2) sl.printnames()
} else if (argtype(1)==1) sl = $o1
}
if (object_id(sl) != 0) {
forsec sl for (x,0) {
if (ismembrane("ca_ion")) {
A += area(x)
ci += cai*area(x)
}
}
} else {
forall for (x,0) {
if (ismembrane("ca_ion")) {
A += area(x)
ci += cai*area(x)
}
}
}
return ci/A
}
func ri2() {local val,i localobj sref
// val = ri2( void )
// measures the sum axial resistance (MΩ) between cas and its adjacent section
val=0
sref = new SectionRef()
if (sref.has_parent()) val=ri(0.5)
for i= 0,sref.nchild-1 {
sref.child[i] val+=ri(0.5)
}
}
func lambda() { local val, rm
if (numarg() > 0) rm = $1 else rm = Rm()
val = 0.5*(diam*rm/Ra)^0.5
return val
}
proc swapchans() {local g
// swapchans( channel name1, channel name2 )
// swap chans replaces one conductance with another maintaining gmax in each section
forall {
strtmp = $s1
if (ismembrane(strtmp)) {
sprint(tmpstr, "g = gmax_%s", strtmp)
execute(tmpstr)
sprint(tmpstr, "uninsert %s", strtmp)
execute(tmpstr)
strtmp = $s2
sprint(tmpstr, "insert %s", strtmp)
execute(tmpstr)
sprint(tmpstr, "gmax_%s = g", strtmp)
execute(tmpstr)
}
}
}
proc ZDirect_g() { local argn,zf,grel,reps,pwr,g,zo,i,max,glim localobj z1, z2, chan, sl
// ZDirect_g( section list, ref section, chan, grel, frequency, reps,pwr,glim,max change)
zf=0
grel=1e-5
reps=0
pwr=1
max=1e2
glim=1
finitialize(v_init)
argn=numarg()
chan = new String()
if (argtype(1)==2) {
sl = new SectionList()
forall ifsec $s1 sl.append()
if (verbosity > 2) sl.printnames()
} else if (argtype(1)==1) sl = $o1
if (argn>1) {
if (argtype(2)==2) {
sprint(tmpstr, "%s sref = new SectionRef()", $s2)
if (verbosity > 2) printf("%s\n", tmpstr)
execute(tmpstr)
}
if (argtype(2)==1) sref = $o2
} else sref = new SectionRef()
if (argn>2) {
if (argtype(3)==2) chan.s = $s3
if (argtype(3)==1) chan = $o3
}
if (argn>3) grel =$4
if (argn>4) zf = $5
if (argn>5) reps =$6
if (argn>6) pwr = $7
if (argn>7) glim =$8
if (argn>8) max = $9
for i=0,reps {
z1 = new Impedance()
z2 = new Impedance()
sref.sec { z1.loc(0.5) }
z1.compute(zf)
forsec sl {
if (ismembrane(chan.s)) {
z2.loc(0.5)
z2.compute(zf)
sref.sec zo = z2.ratio(0.5)
if (grel == 0) {
sprint(tmpstr, "val = gmax_%s", chan.s)
execute(tmpstr)
g = val+max*val*(zo-z1.ratio(0.5)^pwr)
} else g = grel+max*grel*(zo-z1.ratio(0.5)^pwr)
if (verbosity > 3) printf("%g", g)
if (g<1e-9) g=0
if (g>glim) g=glim
sprint(tmpstr, "gmax_%s = %g", chan.s, g)
execute(tmpstr)
}
}
}
}
proc Zratio_g() { local zf,grel,up,pwr,max,g localobj zz, chan, sl
// Zratio_g( section list, init section, chan, grel, frequency, increase/decrease, pwr, max )
//
// sets conductance within sections based on the ratio of impedance between that section
// and a reference location
zf=100
grel=1e-5
up=1
pwr=1
max=1
finitialize(v_init)
chan = new String()
if (argtype(1)==2) {
sl = new SectionList()
forall ifsec $s1 sl.append()
if (verbosity > 3) sl.printnames()
} else if (argtype(1)==1) sl = $o1
if (numarg()>1) {
if (argtype(2)==2) {
sprint(tmpstr, "%s sref = new SectionRef()", $s2)
if (verbosity > 2) printf("%s\n", tmpstr)
execute(tmpstr)
}
if (argtype(2)==1) sref = $o2
} else sref = new SectionRef()
if (numarg()>2) {
if (argtype(3)==2) chan.s = $s3
if (argtype(3)==1) chan = $o3
if (verbosity > 2) printf("%s\n", chan.s)
}
if (numarg()>3) grel = $4
if (numarg()>4) zf = $5
if (numarg()>5) up = $6
if (numarg()>6) pwr = $7
if (numarg()>7) max = $8
//if (msplit) stopPar()
if (verbosity > 2) printf("grel = %g\tzf = %g\tup = %g\tpwr = %g\tmax = %g\n", grel,zf,up,pwr,max)
zz = new Impedance()
sref.sec { zz.loc(0.5) }
zz.compute(zf)
forsec sl {
if (ismembrane(chan.s)) {
if (grel == 0) {
if (strcmp(chan.s, "pas") ==0) { sprint(tmpstr, "val = g_%s", chan.s)
} else sprint(tmpstr, "val = gmax_%s", chan.s)
execute(tmpstr)
} else val = grel
if (up==0) {
g = val*zz.ratio(0.5)^pwr
if (g>max) g=max
if (strcmp(chan.s, "pas") ==0) { sprint(tmpstr, "g_pas = %g", g)
} else sprint(tmpstr, "gmax_%s = %g", chan.s, g)
}
if (up>0) {
g = val/zz.ratio(0.5)^pwr
if (g>max) g=max
if (strcmp(chan.s, "pas") ==0) { sprint(tmpstr, "g_pas = %g", g)
} else sprint(tmpstr, "gmax_%s = %g", chan.s, g)
}
execute(tmpstr)
}
}
}
proc handle_M_dist() { local D, Mhalf, MS, Mmax, Mmin, ms
Mmax = SIZM
Mmin = SIZM/10
Mhalf = 150
MS = -75
ms = issplit()
if (ms) stopPar()
soma[0] {distance()}
forsec "Handle" {
D = distance(0.5)
gmax_M = (Mmin + (Mmax-Mmin)/(1+exp((Mhalf-D)/MS)))
}
if (ms) startPar()
}
proc handle_Na_dist() { local D, d_half, Namax, S, Namin, ms
Namin = SIZNa/50
Namax = SIZNa
d_half = 135
S = -135
ms = issplit()
if (ms) stopPar()
soma[0] {distance()}
forsec "Handle" {
D = distance(0.5)
gmax_Na = (Namin + (Namax-Namin)/(1+exp((d_half-D)/S)))
}
if (ms) startPar()
}
proc gvecSetup() {local gi localobj objtmp, gvec
// gvecSetup(gvec list, conductance/current flag)
// 1st input is list object that the recorded vectors will be added to
// 2nd input specifies whether to record conductance (0) or current (1)
CleanStepEvent()
if (numarg()>1) gi=$2 else gi=0
idc_G_[1] = new IClamp()
Tines[1] idc_G_[1].loc(0.5)
idc_G_[1].amp=0
if (numarg() > 0) {
if (argtype(1)==1) gvec = $o1 else gvec = gvec_G_
} else gvec = gvec_G_
if (SectionListCount(HList)>0) {
if (strcmp(Hname,"hcn") == 0) {
Hchan = new hcnAnalysis()
} else if (strcmp(Hname,"h") == 0) {
Hchan = new hAnalysis()
}
objtmp = new Vector()
if (gi==0) {
objtmp.record(idc_G_[1], &Hchan.nh, RecDt)
objtmp.label("nh")
} else if (gi==1) {
objtmp.record(idc_G_[1], &Hchan.ih, RecDt)
objtmp.label("Ih")
}
gvec.append(objtmp)
}
if (SectionListCount(MList)>0) {
Mchan = new MAnalysis()
objtmp = new Vector()
if (gi==0) {
objtmp.record(idc_G_[1], &Mchan.nM, RecDt)
objtmp.label("nM")
} else if (gi==1) {
objtmp.record(idc_G_[1], &Mchan.iM, RecDt)
objtmp.label("IM")
}
gvec.append(objtmp)
}
if (SectionListCount(KDList)>0) {
if (strcmp(KDname,"KD") == 0) {
KDchan = new KDAnalysis()
} else if (strcmp(KDname,"KD_ca3") == 0) {
KDchan = new KD3Analysis()
}
objtmp = new Vector()
if (gi==0) {
objtmp.record(idc_G_[1], &KDchan.nKD, RecDt)
objtmp.label("nKD")
gvec.append(objtmp)
objtmp = new Vector()
objtmp.record(idc_G_[1], &KDchan.lKD, RecDt)
objtmp.label("lKD")
} else if (gi==1) {
objtmp.record(idc_G_[1], &KDchan.iKD, RecDt)
objtmp.label("IKD")
}
gvec.append(objtmp)
}
if (SectionListCount(KAList)>0) {
KAchan = new KAAnalysis()
objtmp = new Vector()
if (gi==0) {
objtmp.record(idc_G_[1], &KAchan.nKA, RecDt)
objtmp.label("nKA")
gvec.append(objtmp)
objtmp = new Vector()
objtmp.record(idc_G_[1], &KAchan.lKA, RecDt)
objtmp.label("lKA")
} else if (gi==1) {
objtmp.record(idc_G_[1], &KAchan.iKA, RecDt)
objtmp.label("IKA")
}
gvec.append(objtmp)
}
if (SectionListCount(CaTList)>0) {
CaTchan = new CaTAnalysis()
objtmp = new Vector()
if (gi==0) {
objtmp.record(idc_G_[1], &CaTchan.sCaT, RecDt)
objtmp.label("sCaT")
gvec.append(objtmp)
objtmp = new Vector()
objtmp.record(idc_G_[1], &CaTchan.hCaT, RecDt)
objtmp.label("hCaT")
} else if (gi==1) {
objtmp.record(idc_G_[1], &CaTchan.iCaT, RecDt)
objtmp.label("ICaT")
}
gvec.append(objtmp)
}
}
proc SaveSections() { local i, opt
// SaveSections( flag, sections )
opt=0
if ( (object_id(simsecs_G_,1) == -1) && (numarg()==0) ) {
opt = 1
seclist=1
}
if (numarg()>0) {
if (argtype(1)==0) {
opt = 1
seclist=$1
}
}
if ( numarg() > 1 ) opt = 2
if (object_id(simsecs_G_,1) == -1) simsecs_G_ = new SectionList()
if ( opt == 0 ) {
if (verbosity > 1) printf("SaveSections: simsecs_G_ unchanged\n")
return
} else if (opt == 1) {
simsecs_G_ = new SectionList()
if (seclist==1) {
MakeSecList(simsecs_G_,"soma[0]","Handle[25]","MainTrunk[0]","FieldC[15]","FieldB[15]","Tines[287]",\
"Tines[7]","Tines[621]","Tines[786]","Tines[1080]","Tines[895]")
} else if (seclist==2) {
MakeSecList(simsecs_G_,"Handle[40]","MainTrunk[0]","FieldC[25]","Tines[786]","Tines[0]")
} else if (seclist==3) {
MakeSecList( simsecs_G_, "MainTrunk[0]" )
} else if (seclist==4) {
MakeSecList(simsecs_G_,"MainTrunk[0]","Tines[287]","MainTrunk[40]",\
"Tines[7]","Tines[786]","Tines[1080]","Handle[20]")
} else if (seclist==5) {
if (AEC) {
MakeSecList(simsecs_G_,"soma[0]","Tine0[7]","Tine3[6]")//,"Tine5[5]")//,\
// "Tine8[8]","Tine17[4]")
} else MakeSecList(simsecs_G_,"soma[0]","Handle[20]","MainTrunk[0]","Tines[530]","Tines[17]","Tines[621]",\
"Tines[1101]","Tines[1200]","Tines[795]")
}
} else if (opt==2) {
if (argtype(2)==1) {
simsecs_G_ = $o2
} else if (argtype(2)==2) {
for i=2,numarg() {
forall ifsec $si simsecs_G_.append()
}
} else {
if (verbosity > 0) printf("SaveSections: Sections must be specified as string or SectionList\nsimsecs_G_ unchanged\n")
}
}
}