// ----------------------------------------------------------------------------
// membrane.hoc
// loads the full cell morphology, inserts passive
// membrane properties, corrects membrane resistance
// and capacitance for spines, and corrects (roughly)
// for temperature if needed
//
// 2007-06-17, Christoph Schmidt-Hieber, University of Freiburg
//
// accompanies the publication:
// Schmidt-Hieber C, Jonas P, Bischofberger J (2007)
// Subthreshold Dendritic Signal Processing and Coincidence Detection
// in Dentate Gyrus Granule Cells. J Neurosci 27:8430-8441
//
// send bug reports and suggestions to christoph.schmidt-hieber@uni-freiburg.de
//
// 2007-08-31: adheres to NetworkReadyCell policy
//
// ----------------------------------------------------------------------------
// load gui or stdrun:
load_file("stdrun.hoc")
load_file("./../share/genutils.hoc")
load_file("./../share/calcSpines.hoc")
load_file("./../share/fixnseg.hoc")
begintemplate cell_1
public is_art
public init, topol, basic_shape, subsets, geom, biophys, geom_nseg, biophys_inhomo
public synlist, x, y, z, position, connect2target
public somaLoc,distalDendLoc,proxDendLoc,synDendLoc,spineCount,n_sections,n_axon
public section
public all,den,axo
external verbose,debug_mode,accuracy,calc_spines
external q10_cm,q10_g_pas,q10_Ra,tempScale,geom_nseg_shared,lambda_f
objref somaLoc,distalDendLoc,proxDendLoc,synDendLoc,spineCount,this,synlist
proc init() {
topol()
if (debug_mode) print "Loaded cell, n_sections=",n_sections
subsets()
geom()
biophys()
geom_nseg()
synlist = new List()
synapses()
x = y = z = 0 // only change via position
}
// dummy compartments, will be updated later:
create section[1]
proc init_spines() {
forall insert spines
section[0] scale_spines = 1
section[1] scale_spines = 1
section[2] scale_spines = 1.411094934
section[3] scale_spines = 1.416883748
section[4] scale_spines = 1.729047321
section[5] scale_spines = 2.069020426
section[6] scale_spines = 2.326445289
section[7] scale_spines = 2.240566878
section[8] scale_spines = 2.247047065
section[9] scale_spines = 1.587543094
section[10] scale_spines = 2.176976844
section[11] scale_spines = 2.306580816
section[12] scale_spines = 2.465495602
section[13] scale_spines = 2.302641477
section[14] scale_spines = 1.578706329
section[15] scale_spines = 2.281395846
section[16] scale_spines = 2.723287111
section[17] scale_spines = 2.592732622
section[18] scale_spines = 2.057561119
section[19] scale_spines = 2.607937017
section[20] scale_spines = 2.290552402
section[21] scale_spines = 1.400969731
section[22] scale_spines = 1.838412712
section[23] scale_spines = 1.477322919
section[24] scale_spines = 1.601159698
section[25] scale_spines = 2.083591581
section[26] scale_spines = 2.141902991
section[27] scale_spines = 2.209160604
section[28] scale_spines = 2.066909863
section[29] scale_spines = 1.680521809
section[30] scale_spines = 2.387833318
section[31] scale_spines = 2.053749875
section[32] scale_spines = 2.754637153
section[33] scale_spines = 2.584118369
section[34] scale_spines = 1
}
proc init_pas() {
forall {
insert pas
e_pas=0
cm = 0.893279 * tempScale(q10_cm) * scale_spines
g_pas = 2.54178e-05 * tempScale(q10_g_pas) * scale_spines
Ra = 225.066 * tempScale(q10_Ra)
}
}
proc basic_shape() {localobj morphoFile
strdef sectionLabel
morphoFile = new File()
morphoFile.ropen("./cell_1/morpho.txt")
n_sections = morphoFile.scanvar()
create section[n_sections]
n_axon = morphoFile.scanvar()
forall pt3dclear()
while (!morphoFile.eof()) {
morphoFile.scanstr(sectionLabel)
if (strcmp(sectionLabel,"S")==0) {
section[morphoFile.scanvar()] pt3dadd(morphoFile.scanvar(),morphoFile.scanvar(),morphoFile.scanvar(),morphoFile.scanvar())
} else {
print "Unknown section label, aborting file import"
return
}
}
morphoFile.close()
// define soma:
section[0] somaLoc = new Location(0.5)
// define dendritic sites:
section[8] distalDendLoc = new Location(0.8)
section[3] proxDendLoc = new Location(0.05)
section[27] synDendLoc = new Location(0.8)
access somaLoc.secRef.sec
}
proc topol() {
basic_shape()
connect section[1](0), section[0](0)
connect section[2](0), section[1](1)
connect section[3](0), section[2](1)
connect section[4](0), section[3](1)
connect section[5](0), section[4](1)
connect section[6](0), section[5](1)
connect section[7](0), section[5](1)
connect section[8](0), section[4](1)
connect section[9](0), section[3](1)
connect section[10](0), section[9](1)
connect section[11](0), section[10](1)
connect section[12](0), section[10](1)
connect section[13](0), section[9](1)
connect section[14](0), section[2](1)
connect section[15](0), section[14](1)
connect section[16](0), section[15](1)
connect section[17](0), section[15](1)
connect section[18](0), section[14](1)
connect section[19](0), section[18](1)
connect section[20](0), section[18](1)
connect section[21](0), section[1](1)
connect section[22](0), section[21](1)
connect section[23](0), section[21](1)
connect section[24](0), section[23](1)
connect section[25](0), section[24](1)
connect section[26](0), section[25](1)
connect section[27](0), section[25](1)
connect section[28](0), section[24](1)
connect section[29](0), section[23](1)
connect section[30](0), section[29](1)
connect section[31](0), section[29](1)
connect section[32](0), section[31](1)
connect section[33](0), section[31](1)
connect section[34](0), section[0](1)
init_spines()
}
objref all,den,axo
proc subsets() { local i
objref all,den,axo
all = new SectionList()
for i=0, n_sections-1 section[i] all.append()
den = new SectionList()
for i=1, n_sections-1 if (i != 34) section[i] den.append()
axo = new SectionList()
section[34] axo.append()
}
proc geom() {
}
proc geom_nseg() {
geom_nseg_shared()
// increase nseg even further (tribute to Josef):
if (accuracy >= 1) {
forall nseg*=3
}
}
proc biophys() {
init_pas()
}
proc biophys_inhomo(){}
proc position() { local i
somaLoc.secRef.sec for i = 0, n3d()-1 {
pt3dchange(i, $1-x+x3d(i), $2-y+y3d(i), $3-z+z3d(i), diam3d(i))
}
x = $1 y = $2 z = $3
}
obfunc connect2target() { localobj nc //$o1 target point process, optional $o2 returned NetCon
section[34] nc = new NetCon(&v(0), $o1)
nc.threshold = 10
if (numarg() == 2) { $o2 = nc } // for backward compatibility
return nc
}
objref syn_
proc synapses() {
}
func is_art() { return 0 }
endtemplate cell_1