# Overview NEURON Mod Files from the Paper Miceli et al, Genotype-phenotype correlations in neonatal epilepsies caused by mutations in the voltage sensor of Kv7.2 potassium channel subunits, *PNAS* 2013 Feb 25. [Epub ahead of print] In this paper, functional studies revealed that in homomeric or heteromeric configuration with KV7.2 and/or KV7.3 subunits, R213W and R213Q mutations markedly destabilized the open state, causing a dramatic decrease in channel voltage sensitivity. Modeling these channels in CA1 hippocampal pyramidal cells revealed that both mutations increased cell firing frequency, with the R213Q mutation prompting more dramatic functional changes compared with the R213W mutation. The `fig4a.hoc` reproduces the traces shown in Fig. 4a of the paper. ## Instructions for Running the Model ### Under Linux/Unix Systems: 1. To compile the mod files, use the command: ```bash nrnivmodl ``` 2. To run the simulation hoc file, use the command: ```bash nrngui fig6a.hoc ``` ### Under Windows Systems: 1. To compile the mod files, use the `mknrndll` command. 2. A double click on the simulation file `fig6a.hoc` will open the simulation window. ### Under MAC OS X: 1. To compile the mod files, drag and drop the extracted folder onto the mknrndll icon. 2. Drag and drop the mosinit.hoc file onto the nrngui icon. Once the simulation is started you can select the desired simulation. Questions on how to use this model should be directed to michele.migliore@cnr.it # Changelog | Date | Update Details | |------------------------|--------------------------------------------------------------------------------------------------------------------------------------------------------------| | 20150524 | Update from Ted Carnevale: In `cad.mod`, fixed `ca` initialization by inserting `cai = ca` into the `INITIAL` block. Changed integration method from `euler` to `derivimplicit`, which is appropriate for simple ion accumulation mechanisms. See [Integration methods for SOLVE statements](http://www.neuron.yale.edu/phpBB/viewtopic.php?f=28&t=592). | | 20250525 | Readme reformatted as markdown.|