from pylab import *
import scipy.io
import pickle
from os.path import exists
from matplotlib.collections import PatchCollection
import scipy.stats
def mystr(x):
mystr = str(x)
if '000000' in mystr or '999999' in mystr:
for q in range(7,0,-1):
mystr = ('{:.'+str(q)+'f}').format(x)
ilast = len(mystr)-1
if 'e' in mystr:
ilast = mystr.find('e') - 1
if mystr[ilast] != '0':
return mystr
return mystr
def myscistr(x):
mystr = '{:e}'.format(x)
if '0000' in mystr or '9999' in mystr:
for q in range(7,0,-1):
mystr = ('{:.'+str(q)+'e}').format(x)
ilast = mystr.find('e') - 1
if mystr[ilast] != '0':
return mystr
return mystr
def discontlog(ax,x,y,width=18,height=1.05,col='#000000'):
ax.plot([x-width/2,x+width/2],[y/sqrt(height),y*sqrt(height)],'k-',lw=2.3,clip_on=False,color=col)
ax.plot([x-width,x+width],[y/height,y*height],'w-',lw=1.0,clip_on=False,zorder=100)
f,axarr = subplots(7,1)
for iax in range(0,7):
axarr[iax].set_position([0.1+0.07*iax,0.1,0.07,0.1])
for tick in axarr[iax].xaxis.get_major_ticks() + axarr[iax].yaxis.get_major_ticks():
tick.label.set_fontsize(3.5)
axarr[iax].spines['top'].set_visible(False)
axarr[iax].spines['right'].set_visible(False)
axarr[iax].get_xaxis().tick_bottom()
axarr[iax].get_yaxis().tick_left()
axarr[0].set_position([0.07,0.78,0.4,0.205])
for ix in range(0,4):
axarr[1+ix].set_position([0.07+0.11*ix,0.64,0.03,0.06])
axarr[1+ix].set_xticks([])
for iy in range(0,2):
axarr[1+4+iy].set_position([0.12,0.34-0.3*iy,0.28,0.28])
#axarr[9].set_position([0.31,0.2,0.2,0.18])
#axarr[10].set_position([0.55,0.2,0.2,0.18])
#axarr[11].set_position([0.79,0.2,0.2,0.18])
#for i in range(9,12):
# axarr[i].set_visible(False)
for icol in range(0,13):
mychr = hex(15-icol)[2]
mychr2 = hex(icol)[2]
#mycol = "#"+mychr+mychr+mychr2+mychr2+mychr2+mychr2
mycol = "#"+'ff'+mychr2+mychr2+'00'
mycol2 = "#00"+mychr+mychr+"ff"
dists = str(25*icol)+'-'+str(25*(icol+1))+' um'
dists2 = str(100*icol)+'-'+str(100*(icol+1))+' um'
if icol < 11:
polygon = Polygon(array([[-670,-620,-620,-670],[-660+71*icol+x for x in [0,0,71,71]]]).T, True)
p = PatchCollection([polygon], cmap=matplotlib.cm.jet)
p.set_facecolor(mycol)
p.set_edgecolor('none')
axarr[0].add_collection(p)
axarr[0].text(-610,-660+71*icol+35,dists,va='center',ha='left',fontsize=4)
polygon = Polygon(array([[1600,1550,1550,1600],[-660+71*icol+x for x in [0,0,71,71]]]).T, True)
p = PatchCollection([polygon], cmap=matplotlib.cm.jet)
p.set_facecolor(mycol2)
p.set_edgecolor('none')
axarr[0].add_collection(p)
axarr[0].text(1540,-660+71*icol+35,dists2,va='center',ha='right',fontsize=4)
axarr[0].plot([400,600],[200,200],'k-',lw=1.5)
axarr[0].text(500,210,'200 um',va='bottom',ha='center',fontsize=4)
unpicklefile = open('modulhcn_almog/morph_multicolor.sav','rb');morphdata = pickle.load(unpicklefile,encoding='bytes');unpicklefile.close()
for iplotted in range(len(morphdata)-1,-1,-1):
axarr[0].plot(morphdata[iplotted][1],morphdata[iplotted][0],morphdata[iplotted][2],linewidth=morphdata[iplotted][3],color=morphdata[iplotted][4])
unpicklefile = open('modulhcn_hay/morph_multicolor.sav','rb');morphdata = pickle.load(unpicklefile,encoding='bytes');unpicklefile.close()
for iplotted in range(len(morphdata)-1,-1,-1):
axarr[0].plot([0+x for x in morphdata[iplotted][1]], [-500+x for x in morphdata[iplotted][0]],morphdata[iplotted][2],linewidth=morphdata[iplotted][3],color=morphdata[iplotted][4])
axarr[0].axis("equal")
axarr[0].set_ylim([-670,265])
#axarr[0].set_xlabel('$\mu$m',fontsize=6)
#axarr[0].set_ylabel('$\mu$m',fontsize=6,rotation=0)
#axarr[0].spines['top'].set_visible(False)
#axarr[0].spines['right'].set_visible(False)
#axarr[0].get_xaxis().tick_bottom()
#axarr[0].get_yaxis().tick_left()
axarr[0].get_xaxis().set_visible(False)
axarr[0].get_yaxis().set_visible(False)
Ihcoeffs = [0.0,1.0]
#Basal ff, Almog:
cols = ['#0000FF','#000000']
for iIhcoeff in range(0,len(Ihcoeffs)):
Ihcoeff = Ihcoeffs[iIhcoeff]
Ithreshs_thisIh = []
for myseed in range(1,41):
filename = 'modulhcn_almog/ffthreshs/basalffthreshs_Ihcoeff'+str(Ihcoeff)+'_dend0-356_seed'+str(myseed)+'.sav'
if not exists(filename):
print(filename+" does not exist")
continue
unpicklefile = open(filename,'rb');unpickledlist = pickle.load(unpicklefile);unpicklefile.close()
#print(filename+" loaded")
Ithreshs_thisIh.append(unpickledlist[0][-1])
print(str(mean(Ithreshs_thisIh)))
axarr[1].bar(2-iIhcoeff,1e3*mean(Ithreshs_thisIh),facecolor=cols[iIhcoeff]) #convert from uS to nS
print('Basal almog Ihcoeff='+str(Ihcoeff)+' thresh = '+str(1e3*mean(Ithreshs_thisIh)))
#Apical ff, Almog:
cols = ['#0000FF','#000000']
for iIhcoeff in range(0,len(Ihcoeffs)):
Ihcoeff = Ihcoeffs[iIhcoeff]
Ithreshs_thisIh = []
for myseed in range(1,41):
filename = 'modulhcn_almog/ffthreshs/ffthreshs_Ihcoeff'+str(Ihcoeff)+'_apic0-1300_seed'+str(myseed)+'.sav'
if not exists(filename):
print(filename+" does not exist")
continue
unpicklefile = open(filename,'rb');unpickledlist = pickle.load(unpicklefile);unpicklefile.close()
#print(filename+" loaded")
Ithreshs_thisIh.append(unpickledlist[0][-1])
print(str(mean(Ithreshs_thisIh)))
axarr[2].bar(2-iIhcoeff,1e3*mean(Ithreshs_thisIh),facecolor=cols[iIhcoeff]) #convert from uS to nS
print('Apical almog Ihcoeff='+str(Ihcoeff)+' thresh = '+str(1e3*mean(Ithreshs_thisIh)))
#Basal ff, Hay:
cols = ['#0000FF','#000000']
for iIhcoeff in range(0,len(Ihcoeffs)):
Ihcoeff = Ihcoeffs[iIhcoeff]
Ithreshs_thisIh = []
for myseed in range(1,41):
filename = 'modulhcn_hay/ffthreshs/basalffthreshs_Ihcoeff'+str(Ihcoeff)+'_dend0-282_seed'+str(myseed)+'.sav'
if not exists(filename):
print(filename+" does not exist")
continue
unpicklefile = open(filename,'rb');unpickledlist = pickle.load(unpicklefile);unpicklefile.close()
#print(filename+" loaded")
Ithreshs_thisIh.append(unpickledlist[0][-1])
print(str(mean(Ithreshs_thisIh)))
axarr[3].bar(2-iIhcoeff,1e3*mean(Ithreshs_thisIh),facecolor=cols[iIhcoeff]) #convert from uS to nS
print('Basal Hay Ihcoeff='+str(Ihcoeff)+' thresh = '+str(1e3*mean(Ithreshs_thisIh)))
#Apical ff, Hay:
cols = ['#0000FF','#000000']
for iIhcoeff in range(0,len(Ihcoeffs)):
Ihcoeff = Ihcoeffs[iIhcoeff]
Ithreshs_thisIh = []
for myseed in range(1,41):
filename = 'modulhcn_hay/ffthreshs/ffthreshs_Ihcoeff'+str(Ihcoeff)+'_apic0-1300_seed'+str(myseed)+'.sav'
if not exists(filename):
print(filename+" does not exist")
continue
unpicklefile = open(filename,'rb');unpickledlist = pickle.load(unpicklefile);unpicklefile.close()
#print(filename+" loaded")
Ithreshs_thisIh.append(unpickledlist[0][-1])
print(str(mean(Ithreshs_thisIh)))
axarr[4].bar(2-iIhcoeff,1e3*mean(Ithreshs_thisIh),facecolor=cols[iIhcoeff]) #convert from uS to nS
print('Apical Hay Ihcoeff='+str(Ihcoeff)+' thresh = '+str(1e3*mean(Ithreshs_thisIh)))
titles_bars = ['Hay, basal','Hay, apical','Almog, basal','Almog,apical']
for ix in range(0,4):
my_xlim = axarr[1+ix].get_xlim()
my_ylim = axarr[1+ix].get_ylim()
axarr[1+ix].text(mean(my_xlim),my_ylim[1]*1.29,titles_bars[ix],fontsize=5,ha='center',va='center',clip_on=False)
axarr[1+ix].set_ylabel('$g$ (nS)',fontsize=5)
#Apical, givendists, Almog+Hay:
dist1s = [200,300,400,500,600,700,800,900,1000,1100,1200,1300]
treename = 'apic'
for imodel in range(0,2):
minmaxes = [inf,-inf]
coeffs_saved = [[],[]]
for idist1 in range(0,len(dist1s)):
dist1 = dist1s[idist1]
for idist2 in range(0,len(dist1s)):
dist2 = dist1s[idist2]
threshEcons_thisdist2 = []
for iIhcoeff in range(0,2):
Ihcoeff = Ihcoeffs[iIhcoeff]
foundOne = 0
threshEcons = []
for myseed in range(1,41):
filename = 'modulhcn_almog/ffthreshs/ffthreshs_Ihcoeff'+str(Ihcoeff)+'_'+treename+str(dist1)+'-'+str(dist2)+'_seed'+str(myseed)+'.sav'
if imodel == 1:
filename = 'modulhcn_hay/ffthreshs/ffthreshs_Ihcoeff'+str(Ihcoeff)+'_'+treename+str(dist1)+'-'+str(dist2)+'_seed'+str(myseed)+'.sav'
if exists(filename):
unpicklefile = open(filename,'rb')
unpickledlist = pickle.load(unpicklefile)
unpicklefile.close()
threshEcons.append(unpickledlist[0][2])
foundOne = 1
#if not foundOne:
# print(filename+' does not exist')
threshEcons_thisdist2.append(threshEcons[:])
if len(threshEcons) == 0:
continue
c = tanh(mean(threshEcons[:])/5e-5)
minmaxes = [min(minmaxes[0],mean(threshEcons[:])),max(minmaxes[1],(mean(threshEcons[:]) if mean(threshEcons[:]) < 0.0999 else -inf))]
myhex = hex(255-int(255*c))
if len(myhex) < 3:
mycol = '#000000'
elif len(myhex) < 4:
mycol = '#'+'0'+myhex[2]+'0'+myhex[2]+'0'+myhex[2]
elif len(myhex) > 4:
mycol = '#000000'
else:
mycol = '#'+myhex[2:]+myhex[2:]+myhex[2:]
if iIhcoeff == 0:
polygon = Polygon(array([[idist1,idist1+1,idist1],[idist2,idist2,idist2+1]]).T, True) #lower left triangle control
else:
polygon = Polygon(array([[idist1,idist1+1,idist1+1],[idist2+1,idist2,idist2+1]]).T, True) #upper right triangle Ih blocked
p = PatchCollection([polygon], cmap=matplotlib.cm.jet)
p.set_facecolor(mycol); p.set_edgecolor('none')
axarr[5+imodel].add_collection(p)
if len(threshEcons_thisdist2) > 1 and len(threshEcons_thisdist2[0]) > 1 and len(threshEcons_thisdist2[1]) > 1:
s1 = mean(threshEcons_thisdist2[0])
s2 = mean(threshEcons_thisdist2[1])
if s1 == s2:
mycol = '#808080'
elif s1 > s2: # more spiking with Ih than without
c = tanh((s1/s2 - 1)/2)
#print('c = '+str(c))
myhex = hex(200-int(200*c))
if len(myhex) < 3:
mycol = '#000000'
elif len(myhex) < 4:
mycol = '#ff'+'0'+myhex[2]+'0'+myhex[2]
elif len(myhex) > 4:
mycol = '#ff0000'
else:
mycol = '#ff'+myhex[2:]+myhex[2:]
coeffs_saved[0].append(s1/s2)
else: # more spiking without Ih than without
c = tanh((s2/s1 - 1)/2)
#print('c = '+str(c))
myhex = hex(200-int(200*c))
if len(myhex) < 4:
mycol = '#0'+myhex[2]+'0'+myhex[2]+'ff'
elif len(myhex) > 4:
mycol = '#0000ff'
else:
mycol = '#'+myhex[2:]+myhex[2:]+'ff'
coeffs_saved[1].append(s1/s2)
#print(mycol)
#print(myhex)
#print('s1 = '+str(s1)+', s2 = '+str(s2))
pval = scipy.stats.ranksums(threshEcons_thisdist2[0], threshEcons_thisdist2[1])[1]
polygon = Polygon(array([[idist2+1,idist2+1,idist2,idist2],[idist1,idist1+1,idist1+1,idist1]]).T, True)
p = PatchCollection([polygon], cmap=matplotlib.cm.jet)
p.set_facecolor(mycol); p.set_edgecolor('none')
axarr[5+imodel].add_collection(p)
if pval < 0.05/66:
axarr[5+imodel].plot(idist2+0.5,idist1+0.5,'k*',lw=0.5,mew=0.5,ms=2.0)
print('minmaxes = '+str(minmaxes))
print('coeffs_saved = '+str(unique(coeffs_saved[0]))+' and '+str(unique(coeffs_saved[1])))
axnew = f.add_axes([0.026,0.01,0.033,0.59])
axnew2 = f.add_axes([0.407,0.01,0.033,0.59])
zs = [5e-6*i for i in range(0,21)]
for iz in range(0,len(zs)):
polygon = Polygon(array([[1,2,2,1],[iz,iz,iz+1,iz+1]]).T, True)
c = tanh(zs[iz]/5e-5)
myhex = hex(int(255-255*c))
if len(myhex) < 4:
mycol = '#'+'0'+myhex[2]+'0'+myhex[2]+'0'+myhex[2]
elif len(myhex) > 4:
mycol = '#000000'
else:
mycol = '#'+myhex[2:]+myhex[2:]+myhex[2:]
print('myhex='+myhex)
p = PatchCollection([polygon], cmap=matplotlib.cm.jet)
p.set_facecolor(mycol); p.set_edgecolor('none')
axnew.add_collection(p)
if iz < len(zs)-1:
axnew.text(0.8,iz+0.5,myscistr(1e3*zs[iz]),fontsize=4,ha='right',va='center') # convert from uS to nS
else:
axnew.text(0.8,iz+0.5,'$\geq$0.1',fontsize=4,ha='right',va='center')
axnew.text(0.8,len(zs)+0.1,'(nS)',fontsize=4,ha='right',va='center')
axnew.set_ylim([0,21])
axnew.set_xlim([0,2])
axnew.get_xaxis().set_visible(False)
axnew.get_yaxis().set_visible(False)
for q in ['top','bottom','left','right']:
axnew.spines[q].set_visible(False)
zs = [1.1]+[1.0+0.5*i for i in range(1,10)]
for iz in range(0,len(zs)):
polygon = Polygon(array([[1,2,2,1],[iz+1,iz+1,iz+2,iz+2]]).T, True)
polygon2 = Polygon(array([[1,2,2,1],[-iz,-iz,-iz-1,-iz-1]]).T, True)
c = tanh((zs[iz]-1)/2)
myhex = hex(200-int(200*c))
print('myhex='+myhex)
if len(myhex) < 3:
mycol = '#000000'
elif len(myhex) < 4:
mycol = '#ff'+'0'+myhex[2]+'0'+myhex[2]
elif len(myhex) > 4:
mycol = '#ff0000'
else:
mycol = '#ff'+myhex[2:]+myhex[2:]
if len(myhex) < 4:
mycol2 = '#0'+myhex[2]+'0'+myhex[2]+'ff'
elif len(myhex) > 4:
mycol2 = '#0000ff'
else:
mycol2 = '#'+myhex[2:]+myhex[2:]+'ff'
p = PatchCollection([polygon], cmap=matplotlib.cm.jet)
p.set_facecolor(mycol); p.set_edgecolor('none')
axnew2.add_collection(p)
if iz < len(zs)-1:
axnew2.text(0.8,iz+1.5,mystr(zs[iz]),fontsize=4,ha='right',va='center')
else:
axnew2.text(0.8,iz+1.5,'>5',fontsize=4,ha='right',va='center')
p = PatchCollection([polygon2], cmap=matplotlib.cm.jet)
p.set_facecolor(mycol2); p.set_edgecolor('none')
axnew2.add_collection(p)
if iz < len(zs)-1:
axnew2.text(0.8,-iz-0.5,mystr(zs[iz]),fontsize=4,ha='right',va='center')
else:
axnew2.text(0.8,-iz-0.5,'>5',fontsize=4,ha='right',va='center')
polygon = Polygon(array([[1,2,2,1],[0,0,1,1]]).T, True)
p = PatchCollection([polygon], cmap=matplotlib.cm.jet)
p.set_facecolor('#808080'); p.set_edgecolor('none')
axnew2.add_collection(p)
axnew2.text(0.5,0.5,'n/a',fontsize=4,ha='center',va='center')
axnew2.set_ylim([-11,12])
axnew2.set_xlim([0,2])
axnew2.get_xaxis().set_visible(False)
axnew2.get_yaxis().set_visible(False)
for q in ['top','bottom','left','right']:
axnew2.spines[q].set_visible(False)
axnew2.text(2.2,2.5,'Threshold larger for $I_h$-blocked',fontsize=4.5,color='#FF0000',ha='left',va='bottom',rotation=90,clip_on=False)
axnew2.text(2.2,-1.0,'Threshold smaller for $I_h$-blocked',fontsize=4.5,color='#0000FF',ha='left',va='top',rotation=90,clip_on=False)
for iax in range(5,7):
axarr[iax].set_xticks([0.5+x for x in range(0,len(dist1s))])
axarr[iax].set_xticklabels([str(d) for d in dist1s],fontsize=4,rotation=20)
axarr[iax].set_yticks([0.5+x for x in range(0,len(dist1s))])
axarr[iax].set_yticklabels([str(d) for d in dist1s],fontsize=4,rotation=20)
#for ix in range(0,2):
# axarr[0,ix].set_xticks([0.5+x for x in range(0,len(dist1s))])
# axarr[0,ix].set_xticklabels([str(d) for d in dist1s],fontsize=4)
# axarr[0,ix].set_yticks([0.5+x for x in range(0,len(dist2s))])
# axarr[0,ix].set_yticklabels([str(d) for d in dist2s],fontsize=4)
# axarr[0,ix].set_xlim([0,len(dist1s)])
# axarr[0,ix].set_ylim([0,len(dist2s)])
for iax in range(0,7):
pos = axarr[iax].get_position()
f.text(pos.x0 - 0.05, pos.y1 - 0.01 + 0.015*(iax > 0 and iax < 5), chr(ord('A')+iax), fontsize=9)
f.savefig("fig4.eps")