from pylab import *
import scipy.io
import pickle
from os.path import exists
from matplotlib.collections import PatchCollection
import scipy.stats
def mystr(x):
mystr = str(x)
if '000000' in mystr or '999999' in mystr:
for q in range(7,0,-1):
mystr = ('{:.'+str(q)+'f}').format(x)
ilast = len(mystr)-1
if 'e' in mystr:
ilast = mystr.find('e') - 1
if mystr[ilast] != '0':
return mystr
return mystr
def myscistr(x):
mystr = '{:e}'.format(x)
if '0000' in mystr or '9999' in mystr:
for q in range(7,0,-1):
mystr = ('{:.'+str(q)+'e}').format(x)
ilast = mystr.find('e') - 1
if mystr[ilast] != '0':
return mystr
return mystr
def discontlog(ax,x,y,width=18,height=1.05,col='#000000'):
ax.plot([x-width/2,x+width/2],[y/sqrt(height),y*sqrt(height)],'k-',lw=2.3,clip_on=False,color=col)
ax.plot([x-width,x+width],[y/height,y*height],'w-',lw=1.0,clip_on=False,zorder=100)
f,axarr = subplots(3,1)
axarr[0].set_position([0.3,0.53,0.4,0.4])
axarr[1].set_position([0.1,0.06,0.4,0.4])
axarr[2].set_position([0.52,0.06,0.4,0.4])
for iax in range(0,3):
for tick in axarr[iax].xaxis.get_major_ticks() + axarr[iax].yaxis.get_major_ticks():
tick.label.set_fontsize(3.5)
axarr[iax].spines['top'].set_visible(False)
axarr[iax].spines['right'].set_visible(False)
axarr[iax].get_xaxis().tick_bottom()
axarr[iax].get_yaxis().tick_left()
colslope = 2e-5
#Apical, givendists, Almog+Hay:
dist1s = [200,300,400,500,600,700,800,900,1000,1100,1200,1300]
treename = 'apic'
Ihcoeffs = [0.0,1.0]
for imodel in range(0,3):
minmaxes = [inf,-inf]
coeffs_saved = [[],[]]
for idist1 in range(0,len(dist1s)):
dist1 = dist1s[idist1]
for idist2 in range(0,len(dist1s)):
dist2 = dist1s[idist2]
threshEcons_thisdist2 = []
for iIhcoeff in range(0,2):
Ihcoeff = Ihcoeffs[iIhcoeff]
Ihmod = 0.0
if imodel > 0 and Ihcoeff == 0.0:
Ihmod = 10.0 if imodel == 1 else -10.0
foundOne = 0
threshEcons = []
for myseed in range(1,41):
if imodel == 0:
filename = 'ffthreshs/ffthreshs_Ihcoeff'+str(Ihcoeff)+'_apicalCaLVAHay_0.003_100.0_dists585-985_'+treename+str(dist1)+'-'+str(dist2)+'_seed'+str(myseed)+'.sav'
else:
if Ihcoeff == 0:
filename = '../modulhcn_hay/ffthreshs/ffthreshs_Ihmod'+str(Ihmod)+'_'+treename+str(dist1)+'-'+str(dist2)+'_seed'+str(myseed)+'.sav'
else:
filename = '../modulhcn_hay/ffthreshs/ffthreshs_Ihcoeff1.0_'+treename+str(dist1)+'-'+str(dist2)+'_seed'+str(myseed)+'.sav'
if exists(filename):
unpicklefile = open(filename,'rb')
unpickledlist = pickle.load(unpicklefile)
unpicklefile.close()
threshEcons.append(unpickledlist[0][2])
foundOne = 1
print('loaded '+filename)
#if not foundOne:
# print(filename+' does not exist')
threshEcons_thisdist2.append(threshEcons[:])
if len(threshEcons) == 0:
continue
c = tanh(mean(threshEcons[:])/colslope)
minmaxes = [min(minmaxes[0],mean(threshEcons[:])),max(minmaxes[1],(mean(threshEcons[:]) if mean(threshEcons[:]) < 0.0999 else -inf))]
myhex = hex(255-int(255*c))
if len(myhex) < 3:
mycol = '#000000'
elif len(myhex) < 4:
mycol = '#'+'0'+myhex[2]+'0'+myhex[2]+'0'+myhex[2]
elif len(myhex) > 4:
mycol = '#000000'
else:
mycol = '#'+myhex[2:]+myhex[2:]+myhex[2:]
if iIhcoeff == 0:
polygon = Polygon(array([[idist1,idist1+1,idist1],[idist2,idist2,idist2+1]]).T, True) #lower left triangle modulated
else:
polygon = Polygon(array([[idist1,idist1+1,idist1+1],[idist2+1,idist2,idist2+1]]).T, True) #upper right triangle control
p = PatchCollection([polygon], cmap=matplotlib.cm.jet)
p.set_facecolor(mycol); p.set_edgecolor('none')
axarr[imodel].add_collection(p)
if len(threshEcons_thisdist2) > 1 and len(threshEcons_thisdist2[0]) > 1 and len(threshEcons_thisdist2[1]) > 1:
s1 = mean(threshEcons_thisdist2[0])
s2 = mean(threshEcons_thisdist2[1])
if s1 == s2:
mycol = '#808080'
elif s1 > s2: # more spiking with Ih than without
c = tanh((s1/s2 - 1)/2)
#print('c = '+str(c))
myhex = hex(200-int(200*c))
if len(myhex) < 3:
mycol = '#000000'
elif len(myhex) < 4:
mycol = '#ff'+'0'+myhex[2]+'0'+myhex[2]
elif len(myhex) > 4:
mycol = '#ff0000'
else:
mycol = '#ff'+myhex[2:]+myhex[2:]
coeffs_saved[0].append(s1/s2)
else: # more spiking without Ih than without
c = tanh((s2/s1 - 1)/2)
#print('c = '+str(c))
myhex = hex(200-int(200*c))
if len(myhex) < 4:
mycol = '#0'+myhex[2]+'0'+myhex[2]+'ff'
elif len(myhex) > 4:
mycol = '#0000ff'
else:
mycol = '#'+myhex[2:]+myhex[2:]+'ff'
coeffs_saved[1].append(s1/s2)
#print(mycol)
#print(myhex)
#print('s1 = '+str(s1)+', s2 = '+str(s2))
pval = scipy.stats.ranksums(threshEcons_thisdist2[0], threshEcons_thisdist2[1])[1]
polygon = Polygon(array([[idist2+1,idist2+1,idist2,idist2],[idist1,idist1+1,idist1+1,idist1]]).T, True)
p = PatchCollection([polygon], cmap=matplotlib.cm.jet)
p.set_facecolor(mycol); p.set_edgecolor('none')
axarr[imodel].add_collection(p)
if pval < 0.05/66:
axarr[imodel].plot(idist2+0.5,idist1+0.5,'k*',lw=0.5,mew=0.5,ms=2.0)
print('minmaxes = '+str(minmaxes))
print('coeffs_saved = '+str(unique(coeffs_saved[0]))+' and '+str(unique(coeffs_saved[1])))
axnew = f.add_axes([0.16,0.49,0.05,0.49])
axnew2 = f.add_axes([0.7,0.49,0.05,0.49])
zs = [2.0e-6*i for i in range(0,21)]
for iz in range(0,len(zs)):
polygon = Polygon(array([[1,2,2,1],[iz,iz,iz+1,iz+1]]).T, True)
c = tanh(zs[iz]/colslope)
myhex = hex(int(255-255*c))
if len(myhex) < 4:
mycol = '#'+'0'+myhex[2]+'0'+myhex[2]+'0'+myhex[2]
elif len(myhex) > 4:
mycol = '#000000'
else:
mycol = '#'+myhex[2:]+myhex[2:]+myhex[2:]
print('myhex='+myhex)
p = PatchCollection([polygon], cmap=matplotlib.cm.jet)
p.set_facecolor(mycol); p.set_edgecolor('none')
axnew.add_collection(p)
if iz < len(zs)-1:
axnew.text(0.8,iz+0.5,myscistr(1e3*zs[iz]),fontsize=4,ha='right',va='center') # convert from uS to nS
else:
axnew.text(0.8,iz+0.5,'$\geq$0.04',fontsize=4,ha='right',va='center')
axnew.set_ylim([0,21])
axnew.set_xlim([0,2])
axnew.get_xaxis().set_visible(False)
axnew.get_yaxis().set_visible(False)
for q in ['top','bottom','left','right']:
axnew.spines[q].set_visible(False)
zs = [1.1]+[1.0+0.5*i for i in range(1,10)]
for iz in range(0,len(zs)):
polygon = Polygon(array([[1,2,2,1],[iz+1,iz+1,iz+2,iz+2]]).T, True)
polygon2 = Polygon(array([[1,2,2,1],[-iz,-iz,-iz-1,-iz-1]]).T, True)
c = tanh((zs[iz]-1)/2)
myhex = hex(200-int(200*c))
print('myhex='+myhex)
if len(myhex) < 3:
mycol = '#000000'
elif len(myhex) < 4:
mycol = '#ff'+'0'+myhex[2]+'0'+myhex[2]
elif len(myhex) > 4:
mycol = '#ff0000'
else:
mycol = '#ff'+myhex[2:]+myhex[2:]
if len(myhex) < 4:
mycol2 = '#0'+myhex[2]+'0'+myhex[2]+'ff'
elif len(myhex) > 4:
mycol2 = '#0000ff'
else:
mycol2 = '#'+myhex[2:]+myhex[2:]+'ff'
p = PatchCollection([polygon], cmap=matplotlib.cm.jet)
p.set_facecolor(mycol); p.set_edgecolor('none')
axnew2.add_collection(p)
if iz < len(zs)-1:
axnew2.text(0.8,iz+1.5,mystr(zs[iz]),fontsize=4,ha='right',va='center')
else:
axnew2.text(0.8,iz+1.5,'>5',fontsize=4,ha='right',va='center')
p = PatchCollection([polygon2], cmap=matplotlib.cm.jet)
p.set_facecolor(mycol2); p.set_edgecolor('none')
axnew2.add_collection(p)
if iz < len(zs)-1:
axnew2.text(0.8,-iz-0.5,mystr(zs[iz]),fontsize=4,ha='right',va='center')
else:
axnew2.text(0.8,-iz-0.5,'>5',fontsize=4,ha='right',va='center')
polygon = Polygon(array([[1,2,2,1],[0,0,1,1]]).T, True)
p = PatchCollection([polygon], cmap=matplotlib.cm.jet)
p.set_facecolor('#808080'); p.set_edgecolor('none')
axnew2.add_collection(p)
axnew2.text(0.5,0.5,'n/a',fontsize=4,ha='center',va='center')
axnew2.set_ylim([-11,12])
axnew2.set_xlim([0,2])
axnew2.get_xaxis().set_visible(False)
axnew2.get_yaxis().set_visible(False)
for q in ['top','bottom','left','right']:
axnew2.spines[q].set_visible(False)
axnew2.text(2.2,3.5,'Threshold larger for $I_h$-modulated',fontsize=4,color='#FF0000',ha='left',va='bottom',rotation=90,clip_on=False)
axnew2.text(2.2,-1.5,'Threshold smaller for $I_h$-modulated',fontsize=4,color='#0000FF',ha='left',va='top',rotation=90,clip_on=False)
for iax in range(0,3):
axarr[iax].set_xticks([0.5+x for x in range(0,len(dist1s))])
axarr[iax].set_xticklabels([str(d) for d in dist1s],fontsize=4,rotation=20)
axarr[iax].set_yticks([0.5+x for x in range(0,len(dist1s))])
if iax < 2:
axarr[iax].set_yticklabels([str(d) for d in dist1s],fontsize=4,rotation=20)
else:
axarr[iax].set_yticklabels([])
#for ix in range(0,2):
# axarr[0,ix].set_xticks([0.5+x for x in range(0,len(dist1s))])
# axarr[0,ix].set_xticklabels([str(d) for d in dist1s],fontsize=4)
# axarr[0,ix].set_yticks([0.5+x for x in range(0,len(dist2s))])
# axarr[0,ix].set_yticklabels([str(d) for d in dist2s],fontsize=4)
# axarr[0,ix].set_xlim([0,len(dist1s)])
# axarr[0,ix].set_ylim([0,len(dist2s)])
for iax in range(0,3):
pos = axarr[iax].get_position()
f.text(pos.x0 - 0.05, pos.y1 - 0.01, chr(ord('A')+iax), fontsize=9)
f.savefig("figS4.eps")