// Starts a NEURON session for testing synaptic interactions between cells.
//
// Network topology:
// cone
// + - / \ +
// Hz -- Hz
// +
//
// All 3 cells are stimulated equally with the intracellular stimulus
load_file("HzCell.hoc")
load_file("Cone.hoc")
// construct cells
objref preCell, postCell, latCell
preCell = new Cone()
postCell = new HzCell()
latCell = new HzCell()
// postCell = new Cone()
access postCell.soma
// excitatory synapse
objref synEx, pcEx
pcEx = new ParallelContext()
postCell.soma synEx = new Synapse(0.5)
preCell.soma pcEx.source_var(&v(0.5), 1)
postCell.soma pcEx.target_var(synEx, &synEx.V_pre, 1)
synEx.v_th = -47
synEx.v_slope = 4.0
synEx.g_max = 0.050
{ pcEx.setup_transfer() }
// inhibitory synapse
objref synIn, pcIn
pcIn = new ParallelContext()
preCell.soma synIn = new Synapse(0.5)
postCell.soma pcIn.source_var(&v(0.5), 2)
preCell.soma pcIn.target_var(synIn, &synIn.V_pre, 2)
synIn.v_th = -47
synIn.v_slope = 4.0
synIn.g_max = -0.050
{ pcIn.setup_transfer() }
// lateral excitatory synapse ->
objref synLat1, pcLat1
pcLat1 = new ParallelContext()
latCell.soma synLat1 = new Synapse(0.5)
postCell.soma pcLat1.source_var(&v(0.5), 3)
latCell.soma pcLat1.target_var(synLat1, &synLat1.V_pre, 3)
synLat1.v_th = -47
synLat1.v_slope = 6
synLat1.g_max = 0.0050
{ pcLat1.setup_transfer() }
// lateral excitatory synapse <-
objref synLat2, pcLat2
pcLat2 = new ParallelContext()
postCell.soma synLat2 = new Synapse(0.5)
latCell.soma pcLat2.source_var(&v(0.5), 4)
postCell.soma pcLat2.target_var(synLat2, &synLat2.V_pre, 4)
synLat2.v_th = -47
synLat2.v_slope = 6
synLat2.g_max = 0.0050
{ pcLat2.setup_transfer() }
// use preCell to +ve feed into lateral cell
objref synDiag, pcDiag
pcDiag = new ParallelContext()
latCell.soma synDiag = new Synapse(0.5)
preCell.soma pcDiag.source_var(&v(0.5), 5)
postCell.soma pcDiag.target_var(synDiag, &synDiag.V_pre, 5)
synDiag.v_th = -47
synDiag.v_slope = 6
synDiag.g_max = 0.050
{ pcDiag.setup_transfer() }
/////////////////////////////////////////////////////////////////////
// stimulus
objref stim[3]
preCell.soma stim[0] = new IClamp(0.5)
postCell.soma stim[1] = new IClamp(0.5)
latCell.soma stim[2] = new IClamp(0.5)
for i=0,2 {
stim[i].del = 1200
stim[i].dur = 1
stim[i].amp = 0.2
}
/////////////////////////////////////////////////////////////////////
// graphing
objref g1, g2, g3
g1 = new Graph(0)
addplot(g1, 0) // GUI to update this graph
g1.view(0, -55, 2000, 10, 255, 77, 450, 300) //xmin ymin xlen ylen ...
g1.addvar("preCell.soma.v(0.5)", 1, 1)
g1.addvar("postCell.soma.v(0.5)", 2, 1)
g2 = new Graph(0)
addplot(g2, 0) // GUI to update this graph
g2.view(0, -0.02, 2000, 0.04, 255, 405, 450, 300) //xmin ymin xlen ylen ...
g2.addvar("synEx.i", 1, 1)
g2.addvar("synIn.i", 2, 1)
g2.addvar("synLat1.i", 3, 1)
g2.addvar("synLat2.i", 4, 1)
g3 = new Graph(0)
addplot(g3, 0) // GUI to update this graph
g3.view(1200, -51, 800, 4, 255, 700, 450, 300) //xmin ymin xlen ylen ...
g3.addvar("postCell.soma.v(0.5)", 2, 1)
// execution
load_file("exp-2cellsSyn.ses")
{ load_file("parcom.hoc") }
ParallelComputeTool[0].nthread(1)
// recording
load_file("util.hoc")
uRecord(&postCell.soma.v(0.5))
proc save() {
uSave("../netConeHz-results/tmp.txt")
printf("INFO: postCell.soma.v(0.5) -> ../netConeHz-results/tmp.txt\n")
}