// The ball and stick model as a template with inputs length and v_init.
begintemplate Cell
public init, biophys
public soma, iseg, axon, dend, spine_head, spine_neck
proc init() {
// $1 is branch length in microns
// $2 is v_init
bLength = $1
voltInput = $2
biophys(bLength,voltInput)
}
create iseg[40]
create axon
create soma
create dend[1]
create spine_head[1]
create spine_neck[1]
create bigdend[3]
proc biophys() {
length = $1 // length of dendrite in microns
v_init = $2 // resting membrane potential
celsius=37 // nominal temperature of simulation
Ri=100 // internal resistivity in ohm-cm
Cm=1.0 // specific membrane capacitance in uF/cm^2
Rm=15000 // specific membrane resistivity in ohm/cm^2
//na_dend=30 // default Na channel density in pS/um^2
//kv_dend=30 // default K channel density in pS/um^2
na_soma=100 // somatic Na channel density in pS/um^2
kv_soma=100 // somatic k channel density in pS/um^2
access soma
distance()
create dend[length]
create spine_head[length]
create spine_neck[length]
soma {
nseg = 3
diam = 10
L = 20
insert na
gbar_na=na_soma
insert kv
gbar_kv=kv_soma
connect iseg[0](0), 0
nao = 150
ek = -96
for i = 0,2 {
connect bigdend[i] (0), 0.5
}
}
for i = 0,2 {
bigdend[i] {
nseg = 21
diam (0:1) = 5:1
L = 600
}
}
for i = 0, 39 {
iseg[i] {
nseg = 1
diam = 2 - (1.5*(i/40))
L = 1
insert na
insert kv
if (i < 5) {
gbar_na = na_soma
gbar_kv = kv_soma
} else {
gbar_na = 8000
gbar_kv = 2000
}
nao = 150
ek = -96
if (i<39) connect iseg[i+1](0), 1
if (i==39) connect axon(0),1
}
}
axon {
nseg = 201
L = 2000
diam = .5
insert na
gbar_na = 300
insert kv
gbar_kv = 60
nao = 150
ek = -96
}
for i = 0, length-1 {
access soma
distance()
access dend[i]
nseg = 1
L = 1
diam = 5 - ((4*i)/(length-1))
if (i == 0) {
connect soma(1), 0
} else {
connect dend[i-1](1), 0
}
//Adding spines for every micron
dend[i] connect spine_neck[i](0),.5
spine_neck[i] connect spine_head[i](0),1
spine_neck[i] {nseg=1 L=1 diam=0.0504573}
spine_head[i] {nseg=1 L=0.5 diam=0.5}
}
forall {
insert pas
g_pas = 1/Rm
cm = Cm
Ra = Ri
e_pas = v_init
}
access axon
}
endtemplate Cell