NEURON {
POINT_PROCESS r5ht3a
POINTER serotonin
RANGE Re, AR, A2R, A3R, Ob, Oi, Ol, C1, C2, AD, A2D, A3D, D
RANGE g, gmax, Ev
NONSPECIFIC_CURRENT i}
UNITS{
(pA) = (picoamp)
(molar) = (1/liter)
(uM) = (micromolar)
(mV) = (millivolt)
(pS) = (picosiemens)
}
PARAMETER {
k11 = 50000000 (/molar)
k12 = 150
k21 = 40000000 (/molar)
k22 = 300
k31 = 30000000 (/molar)
k32 = 450
k41 = 55
k42 = 300
k51 = 165
k52 = 150
o11 = 0.02
o12 = 330
o31 = 18000
o32 = 6500
o41 = 1500
o42 = 350
o51 = 11
o52 = 11500
o61 = 0.01
o62 = 1.2
o71 = 5100
o72 = 5400
d11 = 50000000 (/molar)
d12 = 0.27
d21 = 40000000 (/molar)
d22 = 0.54
d31 = 30000000 (/molar)
d32 = 0.81
d41 = 0.01
d42 = 1.5
gmax = 0.31 (pS) : conductivity
Ev = 28 (mV)
}
ASSIGNED {
v (mV) : voltage
i (pA) : current
g (pS) : conductance
serotonin (uM) : concentration
k1 (/s) : binding
k2 (/s) : binding
k3 (/s) : binding
d1 (/s)
d2 (/s)
d3 (/s)
}
STATE {
Re
AR
A2R
A3R
Ob
Oi
Ol
C1
C2
AD
A2D
A3D
D
}
INITIAL {
Re=1
}
BREAKPOINT {
SOLVE kstates METHOD sparse
g = gmax*(Ol+Oi+Ob)
i = (1e-3) * g * (v - Ev)
}
KINETIC kstates{
k1 = k11*serotonin
k2 = k21*serotonin
k3 = k31*serotonin
d1 = d11*serotonin
d2 = d21*serotonin
d3 = d31*serotonin
~ Re <-> AR (k1, k12)
~ Re <-> D (d41, d42)
~ AR <-> A2R (k2, k22)
~ A2R <-> A3R (k3, k32)
~ A3R <-> Ob (k41, k42)
~ A3R <-> Oi (k51, k52)
~ Ol <-> A3R (o11, o12)
~ Ol <-> C1 (o51, o52)
~ C1 <-> Oi (o41, o42)
~ C1 <-> Ob (o31, o32)
~ A3D <-> Ol (o61, o62)
~ C2 <-> C1 (o71, o72)
~ D <-> AD (d11, d12)
~ AD <-> A2D (d21, d22)
~ A2D <-> A3D (d31, d32)
CONSERVE Re+AR+A2R+A3R+Ob+Oi+Ol+C1+C2+AD+A2D+A3D+D=1
}