NEURON {
POINT_PROCESS p2x2
POINTER patp
RANGE C1, C2, C3, C4, F5, O6, O7, F8
RANGE g, gmax, Ev
NONSPECIFIC_CURRENT i}
UNITS{
(nA) = (nanoamp)
(molar) = (1/liter)
(uM) = (micromolar)
(mV) = (millivolt)
(pS) = (picosiemens)
}
PARAMETER {
kon1 = 15.98 (/uM /s)
kon2 = 16.3 (/uM /s)
kon3 = 11.6 (/uM /s)
koff1 = 0.019 (/s)
koff2 = 380 (/s)
koff3 = 6822 (/s)
delta = 3718 (/s)
gamma = 43.54 (/s)
alfa1 = 1088 (/s)
beta1 = 540 (/s)
alfa2 = 0.246 (/s)
beta2 = 0.033 (/s)
sigma1 = 31.16 (/s)
eps1 = 79 (/s)
sigma2 = 4.53 (/s)
eps2 = 3.20 (/s)
gmax = 32.4 (pS) : conductance
Ev = -40 (mV)
}
ASSIGNED {
v (mV) : voltage
i (nA) : current
g (pS) : conductance
patp (uM) : concentration
k1 (/s) : binding
k2 (/s) : binding
k3 (/s) : binding
}
STATE {
C1
C2
C3
C4
F5
O6
O7
F8
}
INITIAL {
C1=1
}
BREAKPOINT {
SOLVE kstates METHOD sparse
g = gmax*(O6 + O7)*(1e+6)
i = g * (v - Ev)
}
KINETIC kstates{
k1 = kon1*patp
k2 = kon2*patp
k3 = kon3*patp
~ C1 <-> C2 (k1, koff1)
~ C2 <-> C3 (k2, koff2)
~ C3 <-> C4 (k3, koff3)
~ C4 <-> F5 (delta, gamma)
~ F5 <-> O6 (beta1, alfa1)
~ F5 <-> O7 (beta2, alfa2)
~ F8 <-> O6 (eps1, sigma1)
~ F8 <-> O7 (eps2, sigma2)
CONSERVE C1+C2+C3+C4+F5+O6+O7+F8=1
}