NEURON {
POINT_PROCESS p2x3
POINTER patp
RANGE K1, L1, K2, L2, K3, L3, K4, L4, R4, D1, R1, D4, R3, D3, R5, D5, R2, D2, M4, M4, M3, N3, M2, N2, M1, N1
RANGE Re, AR, A2R, A3R, Ro, AD, A2D, A3D, A3Df, D
RANGE g, gmax, Ev, i
NONSPECIFIC_CURRENT i}
UNITS{
(molar) = (1/liter)
(uM) = (micromolar)
(mV) = (millivolt)
(mM) = (millimolar)
(pS) = (picosiemens)
(pA) = (picoamp)
}
PARAMETER {
K1 = 120000 (/mM /s)
L1 = 20 (/s)
K2 = 80000 (/mM /s)
L2 = 40 (/s)
K3 = 40000 (/mM /s)
L3 = 60 (/s)
K4 = 70 (/s)
L4 = 1 (/s)
R4 = 0.00001 (/s)
D4 = 0.00001 (/s)
R3 = 0.00001 (/s)
D3 = 0.00001 (/s)
R2 = 0.00001 (/s)
D2 = 0.2 (/s)
R5 = 0.0001 (/s)
R1 = 0.0002 (/s)
D1 = 0.001 (/s)
D5 = 23 (/s)
N4 = 1 (/s)
M4 = 0.0001 (/mM /s)
N3 = 0.0255 (/s)
M3 = 8000 (/mM /s)
N2 = 0.017 (/s)
M2 = 16000 (/mM /s)
N1 = 0.0085 (/s)
M1 = 24000 (/mM /s)
gmax = 32.4 (mho/cm2) : conductance
Ev = 0 (mV)
}
ASSIGNED {
v (mV) : voltage
i (pA) : current
g (pS) : conductance
patp (uM) : concentration
k1 (/s) : binding
k2 (/s) : binding
k3 (/s) : binding
m1 (/s)
m2 (/s)
m3 (/s)
m4 (/s)
}
STATE {
Re
AR
A2R
A3R
Ro
AD
A2D
A3D
A3Df
D
}
INITIAL {
Re=1
}
BREAKPOINT {
SOLVE kstates METHOD sparse
g = gmax*Ro
i = g * (v - Ev)
}
KINETIC kstates{
k1 = K1*patp
k2 = K2*patp
k3 = K3*patp
m1 = M1*patp
m2 = M2*patp
m3 = M3*patp
m4 = M4*patp
~ Re <-> AR (k1, L1)
~ Re <-> D (D1, R1)
~ AR <-> A2R (k2, L2)
~ AR <-> AD (D2, R2)
~ A2R <-> A3R (k3, L3)
~ A2R <-> A2D (D3, R3)
~ A3R <-> Ro (K4, L4)
~ A3R <-> A3D (D4, R4)
~ Ro <-> A3Df (D5, R5)
~ A3Df <-> A3D (N4, M4)
~ A3D <-> A2D (N2, m3)
~ A2D <-> AD (N2, m2)
~ AD <-> D (N1, m1)
CONSERVE Re+AR+A2R+A3R+Ro+AD+A2D+A3D+A3Df+D=1
}