load_file("nrngui.hoc")
cvode_active(1)
numaxon=1
numsoma=1
numbasal=52
numapical=70
numtrunk=49
xopen("geo5038804.hoc") // geometry file
xopen("fixnseg.hoc")
Rm = 28000
RmDend = Rm
RmSoma = Rm
RmAx = Rm
Cm = 1
CmSoma= Cm
CmAx = Cm
CmDend = Cm
RaAll= 150
RaSoma=150
RaAx = 50
Vrest = -65
dt = 0.1
gna = .025
AXONM = 5
gkdr = 0.01
celsius = 35.0
KMULT = 0.03
KMULTP = 0.03
ghd=0.00005
objref g, b,c, stim, distrx, distry, cdistry, p
forsec "axon" {insert pas e_pas=Vrest g_pas = 1/RmAx Ra=RaAx cm=CmAx}
forsec "soma" {insert pas e_pas=Vrest g_pas = 1/RmSoma Ra=RaSoma cm=CmSoma}
forsec "dendrite"{insert pas e_pas=Vrest g_pas = 1/RmDend Ra=RaAll cm=CmDend}
forsec "user5" {insert pas e_pas=Vrest g_pas = 1/RmDend Ra=RaAll cm=CmDend}
access soma
freq=50
geom_nseg()
tot=0
forall {tot=tot+nseg}
distance()
tstop=20
b = new VBox()
b.intercept(1)
g = new Graph()
g.size(0,tstop,-70,30)
g.addvar("soma.v(0.5)",1,1,2*tstop,0,2)
g.xaxis(1)
c = new Graph()
c.size(0,740,0,100)
c.xaxis(1)
c.exec_menu("10% Zoom out")
c.color(1)
c.label(0.4,0.8," peak AP")
xpanel("")
xbutton("runm ", "runm()")
xpanel()
b.intercept(0)
b.map()
p = new PlotShape()
p.exec_menu("Shape Plot")
p.size(-194.658,304.758,-223.667,609.667)
p.variable("v")
p.show(0)
rel=0.5
soma {
stim= new IClamp(rel)
stim.amp=2.5
stim.dur=1.5
stim.del=1
}
forsec "axon" {
insert nax gbar_nax=gna * AXONM
insert kdr gkdrbar_kdr=gkdr
insert kap gkabar_kap = KMULTP
}
forsec "soma" {
insert hd ghdbar_hd=ghd vhalfl_hd=-73
insert na3 gbar_na3=gna
insert kdr gkdrbar_kdr=gkdr
insert kap gkabar_kap = KMULTP
}
for i=0, numbasal-1 dendrite[i] {
insert hd ghdbar_hd=ghd vhalfl_hd=-73
insert na3 gbar_na3=gna
insert kdr gkdrbar_kdr=gkdr
insert kap gkabar_kap=0
insert kad gkabar_kad=0
for (x) if (x>0 && x<1) { xdist = distance(x)
ghdbar_hd(x) = ghd*(1+3*xdist/100)
if (xdist > 100){
vhalfl_hd=-81
gkabar_kad(x) = KMULT*(1+xdist/100)
} else {
vhalfl_hd=-73
gkabar_kap(x) = KMULTP*(1+xdist/100)
}
}
}
forsec "apical_dendrite" {
insert ds
insert hd ghdbar_hd=ghd
insert na3 gbar_na3=gna
insert kdr gkdrbar_kdr=gkdr
insert kap gkabar_kap=0
insert kad gkabar_kad=0
for (x) if (x>0 && x<1) { xdist = distance(x)
ghdbar_hd(x) = ghd*(1+3*xdist/100)
if (xdist > 100){
vhalfl_hd=-81
gkabar_kad(x) = KMULT*(1+xdist/100)
} else {
vhalfl_hd=-73
gkabar_kap(x) = KMULTP*(1+xdist/100)
}
}
}
forsec "user5" {
insert ds
insert hd ghdbar_hd=ghd
insert na3 gbar_na3=gna
insert kdr gkdrbar_kdr=gkdr
insert kap gkabar_kap=0
insert kad gkabar_kad=0
for (x) if (x>0 && x<1) { xdist = distance(x)
ghdbar_hd(x) = ghd*(1+3*xdist/100)
if (xdist > 100){
vhalfl_hd=-81
gkabar_kad(x) = KMULT*(1+xdist/100)
} else {
vhalfl_hd=-73
gkabar_kap(x) = KMULTP*(1+xdist/100)
}
}
}
proc init() {
t=0
forall {
v=Vrest
if (ismembrane("nax") || ismembrane("na3")) {ena=55}
if (ismembrane("kdr") || ismembrane("kap") || ismembrane("kad")) {ek=-90}
if (ismembrane("hd") ) {ehd_hd=-30}
}
finitialize(Vrest)
fcurrent()
forall {
for (x) {
if (ismembrane("na3")||ismembrane("nax")){e_pas(x)=v(x)+(ina(x)+ik(x))/g_pas(x)}
if (ismembrane("hd")) {e_pas(x)=e_pas(x)+i_hd(x)/g_pas(x)}
}
}
cvode.re_init()
cvode.event(tstop)
access soma
g.begin()
}
proc advance() {
fadvance()
g.plot(t)
g.flush()
p.flush()
doNotify()
}
proc runp() {
stdinit()
continuerun(tstop)
distrx=new Vector()
distry=new Vector()
forsec "apical_dendrite" {
for (x) if (x>0 && x<1) {
if (diam>=0.) {
distrx.append(distance(x))
distry.append(vmax_ds(x)-Vrest)
}
}
}
distry.mark(c,distrx,"O",3,3,2)
distrx=new Vector()
distry=new Vector()
forsec "user5" {
for (x) if (x>0 && x<1) {
if (diam>=0) {
distrx.append(distance(x))
distry.append(vmax_ds(x)-Vrest)
}
}
}
distry.mark(c,distrx,"t",5,$1,1)
c.flush()
doNotify()
}
proc runm() {
runp(1)
}