//L23 V1 pyramidal neuron, Based on Branco 2010, Accession number 140828
//Passive Properties
celsius = 37
v_init =-79
Rm=11000
Ri =100
Cm =1
for i=0, all.count()-1 {
access all.o(i).sec
insert pas
g_pas = 1/Rm
Ra=Ri
}
for i=0, somatic.count()-1 {
access somatic.o(i).sec
cm=Cm
}
for i=0, basal.count()-1 {
access basal.o(i).sec
cm=Cm*2
}
if (!ablated) {
for i=0, apical.count()-1 {
access apical.o(i).sec
cm=Cm*2
}
}
//-------------------------------------------------------
//add active properties
//-------------------------------------------------------
proc init_active_params(){
Ek = -80
Ena = 60
Eca = 140
vshift_na = -5//-5
vshift_ca = 0
ca_factor=0.2
gna_soma =1000*1.1*0.459 //Smith Hausser
gkv_soma = 100 *0.5 //Smith Hausser
gkm_soma = 2.2 *1.27 //Smith,Hausser
gka_soma = 0.003 *1.8 //5% of dendrites-Burkhalter et al. 2006
gkca_soma =3 *0.1 *7 //Smith Hausser
gca_soma = 0.5 *0.5*ca_factor //Cho,2006
git_soma = 0.0003 *0.5 *ca_factor //Cho,2006
gna_dend = 600 *1.1*0.459
gkv_dend = 3 *0.5
gkm_dend = 1 *1.27
gka_dend = 0.06 *1.8
gkca_dend = 3 *0.1 *7
gca_dend = 0.5 *0.5*ca_factor
git_dend = 0.0003 *0.5*ca_factor
}
proc init_active(){
access soma
distance()
for i=0, somatic.count()-1 {
access somatic.o(i).sec
insert na gbar_na = gna_soma
insert kv gbar_kv = gkv_soma
insert km gbar_km = gkm_soma
insert kap gbar_kap= gka_soma
insert kca gbar_kca = gkca_soma
insert ca gbar_ca = gca_soma
insert it gbar_it = git_soma
insert cad
}
for i=0, basal.count()-1 {
access basal.o(i).sec
insert na gbar_na = gna_dend
insert kv gbar_kv = gkv_dend
insert km gbar_km = gkm_dend
insert kca gbar_kca = gkca_dend
insert kap gbar_kap= gka_dend
if (diam>0.8) { //Tuft-Burkhalter et al. 2006
gbar_kap= gka_dend*0.1
} else {
gbar_kap= gka_dend
}
insert ca
insert it
for (x) {
xdist = distance(x)
gbar_ca = gca_dend+(0.002*xdist*gca_dend)
gbar_it = git_dend+(0.002*xdist*git_dend)
}
insert cad
}
if (!ablated) {
for i=0, apical.count()-1 {
access apical.o(i).sec
insert na gbar_na = gna_dend
insert kv gbar_kv = gkv_dend
insert km gbar_km = gkm_dend
insert kca gbar_kca = gkca_dend
insert kap
if (diam>0.8) { //Tuft-Burkhalter et al. 2006
gbar_kap= gka_dend*0.1
} else {
gbar_kap= gka_dend
}
insert ca
insert it
for (x) {
xdist = distance(x)
if (xdist>260) {
gbar_ca = gca_dend*0.4 //Cho et al. 2006
gbar_it = git_dend*0.4
} else {
gbar_ca = gca_dend*(0.9846*sin((0.008758*xdist)+0.8656))
gbar_it = git_dend*(0.9846*sin((0.008758*xdist)+0.8656))
}
}
insert cad
}
}
for i=0, all.count()-1 {all.o(i).sec if(ismembrane("k_ion")) {ek = Ek}}
for i=0, all.count()-1 {all.o(i).sec if(ismembrane("na_ion")) {ena = Ena}}
for i=0, all.count()-1 {
access all.o(i).sec
if(ismembrane("ca_ion")) {
ion_style("ca_ion",0,1,0,0,0)
vshift_ca = 0
}
}
}
init_active_params()
init_active()
xopen("current-balance.hoc")
current_balance(v_init)