• Open the folder "fieldCalc". • Open the file "fieldGUI.m" with MATLAB program. • type "fieldGUI" in the commend window and press enter. • A window named "fieldGUI" will appear. Enter the coil parameters into the three text boxes: o Coil radius in meters. o Number of coiling (loops). o Distance of the cell from the coil (Z) in meters. • Press the "show graph" bottom and two graphs will appear below, the induced electric field in x direction (Ex) on the left and the induced electric field in the y direction of the left (Ey). This will take 60-90 minutes. The matrixes of the two graph will also be saved in the folder "fieldCalc" as text files. • Copy the text files (ex.txt and ey.txt) into the folder named "Neuron". • Open the file " BACModel_mag.hoc" located in the "Neuron" folder. • The first figure that opens shows the soma membrane potential as a function of time. The second shows the pulse shape. • To run the program press the "Init & Run" bottom in the "RunControl" panel. • You can control the electrical circuit and stimulation parameters in the Magnetic Coil Current window. Changing the stimulation amplitude to a value higher than 1900 volt will present a superthreshold activity. • To manufacture a shape plot: o go to NEURON main menu o click on graph - shape plot. A window showing the cell morphology will appear. o Right click in the window and chose the option shape plot. • The cell in the window will be colored according to the membrane potential in every location. A scale bar will also appear next to it, presenting the voltage matching the different colors. To start the movie press the "Init & Run" bottom in the "RunControl" panel.