load_file("nrngui.hoc")
cvode.active(1)
mid=5
dist=27
Rm = 28000
RmDend = Rm/2
RmSoma = Rm
RmAx = Rm
Cm = 1
CmSoma= Cm
CmAx = Cm
CmDend = Cm*2
RaAll= 150
RaSoma=150
RaAx = 50
Vrest = -65
gna = .032
AXONM = 2
gkdr = 0.01
celsius = 34.0
KMULT = 0.048
KMULTP = 0.048
ghd=0.0005
tstop=70
objref nconp, ncond, net, netp, netd, g, b, c, synp, synd, apc
strdef marker
xopen("n160_mod.nrn") // geometry file
b = new VBox()
b.intercept(1)
g = new Graph()
g.size(0,tstop,-70,-50)
g.xaxis(1)
g.label(0.45,0.9,"time (ms)")
g.label(0.45,0.22,"S1")
g.mark(33,-66,"O",6,1,1)
g.exec_menu("10% Zoom out")
c = new Graph()
c.size(-35,30,-2,5)
c.yaxis(3)
c.exec_menu("10% Zoom out")
c.beginline(1,2)
c.line(0,-0.5)
c.line(0,3)
c.label(0.18,0.22,"-30")
c.label(0.52,0.22,"0")
c.label(0.7,0.22,"18")
c.label(0.42, 0.1,"tS2 - tS1 (ms)")
xpanel("",1)
xbutton("full model (black)", "runf()")
xbutton("uniform KA (red)", "runa()")
xbutton("uniform KA and I-h (blue)", "runh()")
xpanel()
b.intercept(0)
b.map()
access soma[5]
distance()
netp = new NetStim(0.5)
netp.number=1
netd = new NetStim(0.5)
netd.number=1
apc = new APCount(0.5)
apc.thresh = 0
apical[mid] {
print distance(0.25)
synp = new Exp2Syn(0.25)
synp.e=0
synp.tau1=3
synp.tau2=3
}
apical[dist] {
print distance(0.5)
synd = new Exp2Syn(0.5)
synd.e=0
synd.tau1=3
synd.tau2=3
}
axon[1] {
insert nax gbar_nax=gna * AXONM
insert kdr gkdrbar_kdr=gkdr
insert pas g_pas = 1/RmAx Ra=RaAx cm=CmAx
insert kap gkabar_kap = KMULTP*0.2
}
axon[0] {
insert nax gbar_nax=gna * AXONM
insert kdr gkdrbar_kdr=gkdr
insert pas g_pas = 1/RmAx Ra=RaAx cm=CmAx
insert kap gkabar_kap = KMULTP*0.2
}
for i=0,NumSoma-1 soma[i] {
insert hd ghdbar=ghd
vhalfl_hd=-82
insert na3 gbar_na3=gna
insert kdr gkdrbar_kdr=gkdr
ar2_na3=0.8
insert kap gkabar_kap = KMULTP
insert pas g_pas = 1/RmSoma Ra=RaSoma cm=CmSoma
}
for i=0,NumBasal-1 basal[i] {
insert na3 gbar_na3=gna
insert kdr gkdrbar_kdr=gkdr
ar2_na3=1
insert kap gkabar_kap=KMULTP
insert pas g_pas = 1/RmDend Ra=RaAll cm=CmDend
}
for i=0,NumApical-1 apical[i] {
insert pas Ra=RaAll g_pas = 1/RmDend cm=CmDend
if (diam>0.5 && distance(0.5)<500) {
insert hd ghdbar_hd=ghd
insert na3
ar2_na3=0.7
gbar_na3=gna
insert kdr
gkdrbar_kdr=gkdr
insert kap
insert kad
gkabar_kap=0
gkabar_kad=0
}
}
nconp= new NetCon(netp,synp,0.5,0,3.6e-3)
ncond= new NetCon(netd,synd,0.5,0,3.5e-3)
proc init() {
t=0
membs = Vrest
for i=0,NumApical-1 apical[i] {
if (diam>0.5 && distance(0.5)<500) {
for (x){ xdist = distance(x)
if (xdist>500) {xdist=500}
if (hflag==0) {ghdbar_hd(x) = ghd*(1+3*xdist/100)} else {ghdbar_hd(x) = ghd}
if (xdist > 100){
vhalfl_hd(x)=-90
if (aflag==0) {gkabar_kad(x) = KMULT*(1+xdist/100)} else {gkabar_kad(x) = KMULT}
} else {
vhalfl_hd(x)=-82
if (aflag==0) {gkabar_kap(x) = KMULTP*(1+xdist/100)} else {gkabar_kap(x) = KMULTP}
}
}
}
}
forall {
v=Vrest
if (ismembrane("nax") || ismembrane("na3")) {ena=55}
if (ismembrane("kdr") || ismembrane("kap") || ismembrane("kad")) {ek=-90}
if (ismembrane("hd") ) {ehd_hd=-30}
}
finitialize(v)
fcurrent()
forall {
for (x) {
e_pas(x)=Vrest
if (ismembrane("hd")) {e_pas(x)=e_pas(x)+i_hd(x)/g_pas(x)}
if (ismembrane("kad")||ismembrane("kap")){e_pas(x)=e_pas(x)+(ina(x)+ik(x))/g_pas(x)}
}
}
cvode.re_init()
access soma[5]
g.begin()
g.plot(t)
}
proc step() {
fadvance()
g.plot(t)
}
proc run() {
init()
while(t<tstop) { step()}
g.flush()
doNotify()
}
proc runf() {
c.begin()
c.exec_menu("Keep Lines")
aflag=0
hflag=0
color=1
loop(3,54, 1)
}
proc runa() {
c.begin()
c.exec_menu("Keep Lines")
aflag=1
hflag=0
color=2
loop(3,54, 1.5)
}
proc runh() {
c.begin()
c.exec_menu("Keep Lines")
aflag=1
hflag=1
color=3
loop(3,54, 2)
}
proc loop() {
c.color(color)
g.addvar("soma[5].v(0)",color,1, 2*tstop,0,2)
netd.start=$1
netp.start=33
while (netd.start<$2) {
run()
if (apc.n==0) {sprint(marker,"o")} else {sprint(marker,"O")}
c.mark(netd.start-netp.start,$3,marker,10,color,1)
doNotify()
netd.start=netd.start+3
}
}