############################# # SYSTEM DEFINING EQUATIONS # ############################# v' = 1000. * V_DOT(v,n,k_so,k_ex,h) n' = 1000. * N_DOT(v,n,k_so,k_ex) k_so' = 1000. * K_SO_DOT(v,n,k_so,k_ex) k_ex' = 1000. * K_EX_DOT(v,n,k_so,k_ex) h' = 1000. * H_DOT(v,h) ###################### # INITIAL CONDITIONS # ###################### # PHYSIOLOGICAL ############### # par KBATH=4 # init v=-68.019025 # init n=0.064944565 # init k_so=130.99026 # init k_ex=3.9992148 # TONIC FIRING ############## # par KBATH=7 # init v=-56.844971 # init n=0.14995722 # init k_so=136.81308 # init k_ex=6.9882756 # SEIZURE-LIKE ############## # kbath: #KBATH=4+20*heav(t-10)*heav(50-t) #par IAPP=0 #par MAX_PUMP=5.2471672 # iapp: #IAPP=50*heav(t-10)*heav(17-t) #par KBATH=4.01 #par MAX_PUMP=5.2471672 # pump: par t1=10 par t2=17.1 par t3=22.1 #par t1=10 #par t2=25 #par t3=30 stim(arg)=heav(t1-arg)*(1-((1-0.2)/t1)*arg)+ 0.2*heav(arg-(t1+0.000001))*heav(t2-arg) + heav(arg-(t2+0.000001))*heav(t3-arg)*(0.2+(1-0.2)/(t3-t2)*(arg-t2))+heav(arg-(t3+0.000001)) MAX_PUMP(t)=5.2471672*stim(t) par KBATH=4.0 par IAPP=0 init v=-67.971672 init n=0.065193631 init k_so=131.07088 init k_ex=4.0102115 init h=0.69483376 # TONIC FIRING ############## # par KBATH=12 # init v=-5.8720033538E+01 # init n=1.3065201430E-01 # init k_so=1.3504571762E+02 # init k_ex=1.1993252621E+01 # PERIODIC SD ############# # par KBATH=17 # init v=-93.984886 # init n=0.0063529861 # init k_so=117.70079 # init k_ex=5.4777171 par F_DIFF=0.05 par Vmax=-66.8091 ########## # GATING # ########## ALPHA_N(v) = 0.01 * (v + 34.0) / (1.0 - exp(-0.1 * (v + 34.0))) BETA_N(v) = 0.125 * exp(-(v + 44.0) / 80.0) ALPHA_M(v) = 0.1 * (v + 30.0) / (1.0 - exp(-0.1 * (v + 30.0))) BETA_M(v) = 4.0 * exp(-(v + 55.0) / 18.0) ALPHA_H(v) = 0.07* exp( -0.05* (v+44)) BETA_H(v) = 1.0 / (1 + exp(-0.1 * (v + 14))) TAU_H1(v) = (1.0/(ALPHA_H(v)+BETA_H(v)))/PHI TAU_H(x) = TAU_H1(x)*(1.335*tanh((x-Vmax)*0.1)+1.665) H_INF(v) = ALPHA_H(V) / (ALPHA_H(v) + BETA_H(v)) M(v) = ALPHA_M(v) / (ALPHA_M(v) + BETA_M(v)) #H(n) = 1 - 1. / (1 + exp(-6.5*(n-0.35))) ###################### # ION CONCENTRATIONS # ###################### par VOL_SO=2.16 par VOL_EX=0.72 par NA_SO_INI=27. par K_SO_INI=130.98993 par NA_EX_INI=120. #par V_INI=-68. NA_SO(k_so) = NA_SO_INI + K_SO_INI - k_so NA_EX(k_so) = (NA_SO_INI * VOL_SO + NA_EX_INI * VOL_EX - NA_SO(k_so) * VOL_SO) / VOL_EX ##################### # NERNST POTENTIALS # ##################### EK(k_so,k_ex) = 26.64 * log(k_ex/k_so) ENA(k_so) = 26.64 * log(NA_EX(k_so)/NA_SO(k_so)) ############ # CURRENTS # ############ par G_NA_L=0.0175 par G_NA_G=100. par G_K_L=0.05 par G_K_G=40. par NA_PUMP=25 par K_PUMP=5.5 I_NA_L(v,k_so) = G_NA_L * (v - ENA(k_so)) I_NA_G(v,n,k_so,h) = G_NA_G * M(v) * M(v) * M(v) * h * (v - ENA(k_so)) I_K_L(v,k_so,k_ex) = G_K_L * (v - EK(k_so,k_ex)) I_K_G(v,n,k_so,k_ex) = G_K_G * n * n * n * n * (v - EK(k_so,k_ex)) IPUMP(k_so,k_ex) = MAX_PUMP(t) / (1.0 + exp((NA_PUMP - NA_SO(k_so))/3.)) / (1. + exp(K_PUMP - k_ex)) I_NA(v,n,k_so,k_ex,h) = I_NA_L(v,k_so) + I_NA_G(v,n,k_so,h) + 3. * IPUMP(k_so,k_ex) I_K(v,n,k_so,k_ex) = I_K_L(v,k_so,k_ex) + I_K_G(v,n,k_so,k_ex) - 2. * IPUMP(k_so,k_ex) JDIFF(k_ex) = F_DIFF * 4. / 3. * (KBATH - k_ex) / 1000. ################## # RATE FUNCTIONS # ################## par PHI=3 par C=1 par CONV=9.55589e-05 V_DOT(v,n,k_so,k_ex,h) = -1. / C * (I_NA(v,n,k_so,k_ex,h) + I_K(v,n,k_so,k_ex)-IAPP) N_DOT(v,n,k_so,k_ex) = PHI * (ALPHA_N(v) * (1 - n) - BETA_N(v) * n) K_SO_DOT(v,n,k_so,k_ex) = -1. / VOL_SO * CONV * I_K(v,n,k_so,k_ex) K_EX_DOT(v,n,k_so,k_ex) = 1. / VOL_EX * CONV * I_K(v,n,k_so,k_ex) + JDIFF(k_ex) H_DOT(v,h) = (1.0 / TAU_H(v)) * (H_INF(v) - h) ############### # AUXILIARIES # ############### #aux _Ina_gl = I_NA_L(v,k_so) + I_NA_G(v,n,k_so,h) #aux _Ik_gl = I_K_L(v,k_so,k_ex) + I_K_G(v,n,k_so,k_ex) aux _ENA = ENA(k_so) aux _EK = EK(k_so,k_ex) aux _stim = stim(t) aux _Ip = IPUMP(k_so,k_ex) ############ # NUMERICS # ############ #@ meth=runge-kutta @ meth=cvode @ dt=1e-4,tol=1e-10,atol=1e-10 @ maxstor=1000000000, bounds=10000000 @ total=100 @ bell=0 ############ # GRAPHICS # ############ @ xhi=100 @ nplot=4, yp1=v, yp2=_EK, yp3=_ENA, yp4=_stim, ylo=-150, yhi=160 #@ nplot=4, yp1=k_so, yp2=k_ex, yp3=_NA_SO, yp4=_NA_EX, ylo=0, yhi=150 done