TITLE large-conductance calcium-activated potassium channel (BK)
:Mechanism according to Gong et al 2001 and Womack&Khodakakhah 2002,
:adapted for Layer V cells on the basis of Benhassine&Berger 2005.
:NB: concentrations in mM
NEURON {
SUFFIX kBK
USEION k READ ek WRITE ik
USEION ca READ cai
RANGE gpeak, gkact, caPh, caPk, caPmax, caPmin
RANGE caVhh, CaVhk, caVhmax, caVhmin, k, tau
}
UNITS {
(mA) = (milliamp)
(mV) = (millivolt)
(molar) = (1/liter)
(mM) = (millimolar)
}
PARAMETER {
:maximum conductance (Benhassine 05)
gpeak = 268e-4 (mho/cm2) <0, 1e9>
: Calcium dependence of opening probability (Gong 2001)
caPh = 2e-3 (mM) : conc. with half maximum open probaility
caPk = 1 : Steepness of calcium dependence curve
caPmax = 1 : max and
caPmin = 0 : min open probability
: Calcium dependence of Vh shift (Womack 2002)
caVhh = 2e-3 (mM) : Conc. for half of the Vh shift
caVhk = -0.94208 : Steepness of the Vh-calcium dependence curve
caVhmax = 155.67 (mV) : max and
caVhmin = -46.08 (mV) : min Vh
: Voltage dependence of open probability (Gong 2001)
: must not be zero
k = 17 (mV)
: Timeconstant of channel kinetics
: no data for a description of a calcium&voltage dependence
: some points (room temp) in Behassine 05 & Womack 02
tau = 1 (ms) <1e-12, 1e9>
scale = 100 : scaling to incorporate higher ca conc near ca channels
}
ASSIGNED {
v (mV)
ek (mV)
ik (mA/cm2)
cai (mM)
caiScaled (mM)
pinf (1)
}
STATE {
p
}
BREAKPOINT {
SOLVE states METHOD cnexp
ik = gpeak*p* (v - ek)
}
DERIVATIVE states {
rate(v, cai)
p' = (pinf - p)/tau
}
INITIAL {
rate(v, cai)
p = pinf
}
PROCEDURE rate(v(mV), ca(mM)) {
caiScaled = ca*scale
pinf = P0ca(caiScaled) / ( 1 + exp( (Vhca(caiScaled)-v)/k ) )
}
FUNCTION P0ca(ca(mM)) (1) {
if (ca < 1E-18) { :check for division by zero
P0ca = caPmin
} else {
P0ca = caPmin + ( (caPmax - caPmin) / ( 1 + (caPh/ca)^caPk ))
}
}
FUNCTION Vhca(ca(mM)) (mV) {
if (ca < 1E-18) { :check for division by zero
Vhca = caVhmax
} else {
Vhca = caVhmin + ( (caVhmax - caVhmin ) / ( 1 + ((caVhh/ca)^caVhk)) )
}
}