// Initialize GUI
objref p
p=new PWManager()
t_stop_temp = tstop
dt_temp = dt
xopen("$(NEURONHOME)/lib/hoc/noload.hoc")
tstop = t_stop_temp
dt = dt_temp
nrnmainmenu()
// Initialize Model
objref somatodendritic, dendritic
/* Global */
celsius = 37
v_init = -70
/* Passive membrane */
ra = 150 // decrease ad to decrease of soma vth variability, increase axon's vth variability
global_ra = ra
rm = 30000 // g_pas=1/rm
c_m = 0.5
cm_myelin = 0.02
g_pas_node = 0.02
/* Active channels */
// Nav
Ena = 60
gna12_soma = 80
gna12_dend = 80
gna12_ais_max = 3200 // Nav1.2
gna16_ais_max = 3200 // Nav1.6
gna16_nakeaxon= 300
gna12_myelin=20 // Nav1.2 at myelins
gna16_node = 3200 // Nav1.6 at node
// usetable
usetable=0
usetable_na=0
usetable_na12=0
usetable_na16=0
usetable_kca=0
usetable_km=0
usetable_kv=0
vhalf_na12 = -30
vhalf_na16 = -43
vhalf_na = -30
vhalf_na12_stoch = -30
vhalf_na16_stoch = -43
vhalf_na_stoch = -30
// Kv
Ek = -90
gkv_soma = 20
gkv_dend = 10
gkv_axon = 1000
// Km
gkm = .3
gkm_soma = gkm
// Kca
gkca = 3
gkca_soma = gkca
// Ca
Eca=140
gca = .3
gca_soma = gca
// gamma
gamma_na = gamma
gamma_na12 = gamma
gamma_na16 = gamma
gamma_na_stoch = gamma
gamma_na12_stoch = gamma
gamma_na16_stoch = gamma
// ------------------------------------------------
// Cell Geometry
// ------------------------------------------------
/* Clean up */
forall delete_section()
/* Soma and Dendrites */
load_file("morphology/soma_dendrites.hoc")
// build a sectionlist for soma and dendrites
somatodendritic = new SectionList()
forall {
if (L/nseg>40) {
nseg = L/40 + 1
} // make sure no segments exceed 40 uM length. Note, soma.nseg remains 10.
somatodendritic.append() // soma and dendrites are all included
}
// build a sectionlist for dendrites only
dendritic = new SectionList()
forsec somatodendritic dendritic.append()
soma dendritic.remove() // remove soma for pure dendritic sectionlist
/* Axon */
axonOnSoma=1
load_file ("morphology/axon.hoc")
create_axon()
/* Spines */
aspiny = 0 // 0 for spiny
if (!aspiny) {
load_file ("morphology/spines.hoc")
add_spines(dendritic,spine_dens)
}
distance(0,axonOnSoma) // set the point where axon seated on soma as the origin
// ----------------------------------------------------
// Insert Density Mechanisms
// ----------------------------------------------------
load_file ("density_mechs.hoc")
// Install passive membrane properties
install_passive()
printf("stoch,%d\n",stoch)
// Install active channels
if(stoch==0){
install_channels()
}
if(stoch==1){
install_channels_stoch()
}
// -----------------------------------------------------------
// Experiments: Insert Point Processes or Something Else
// -----------------------------------------------------------
xopen("recording.hoc")
// Call the Session "hu_all"
xopen ("sess.ses")
/* Where Ini */
xopen("inject_soma.hoc")