//genesis
/*************************** MS Model, Version 5.11 **********************
**************************** MS_cell.g ***************
Tom Sheehan tsheeha2@gmu.edu thsheeha@vt.edu 703-538-8361
*************************
MS_cell.g has only one externally called function: make_MS_cell. That primary
function uses the services of the following two local subroutines:
set_position
add_channels
******************************************************************************/
include MScell/globals // Defines & initializes cell specific parameters
include MScell/addchans // provides access to add_uniform_channel & add_CaShells
// as required by local subroutine add_channels
include MScell/proto // provides access to make_prototypes required by primary
// routine make_MS_cell
//************************ Begin Local Subroutines ****************************
//*****************************************************************************
//************************ Begin function set_position *********************
//**************************************************************************
function set_position (cellpath)
//********************* Begin Local Variables ************************
str compt, cellpath
float dist2soma,x,y,z
//********************* End Local Variables *****************************
if (!{exists {cellpath}})
echo The current input {cellpath} does not exist (set_position)
return
end
foreach compt ({el {cellpath}/##[TYPE=compartment]})
x={getfield {compt} x}
y={getfield {compt} y}
z={getfield {compt} z}
dist2soma={sqrt {({pow {x} 2 }) + ({pow {y} 2}) + ({pow {z} 2})} }
setfield {compt} position {dist2soma}
end
end
//************************ End function set_position ***********************
//**************************************************************************
//************************ Begin function add_channels *********************
//**************************************************************************
function add_channels (cellpath)
str cellpath
/************************************************************************
next, to add ion channels the function "add_uniform_channel" is
called to insert channels in to the cell with the distance to soma
between a(minimum) and b(max) more details can be found in the file
"adjust.g"
MAGIC_NUMBERS_1
However the question remains: where do the values of a, b, & conductance
density come from?
************************************************************************/
/* add_uniform_channel (from addchans.g)
channel_Name a b density */
// Naf in the soma
add_uniform_channel "NaF_channel_D1" 0 16.1e-6 {gNaFprox_D1} {cellpath}
// Naf in the dendrites
add_uniform_channel "NaF_channel_D1" 16.1e-6 90e-6 {gNaFmid_D1} {cellpath}
add_uniform_channel "NaF_channel_D1" 90.0e-6 500e-6 {gNaFdist_D1} {cellpath}
// KaF in the soma and proximal dendrites
add_uniform_channel "KAf_channel" 0 16.1e-6 {gKAfprox} {cellpath}
// KaF in the middel and distal dendrites
add_uniform_channel "KAf_channel" 16.1e-6 60.5e-6 {gKAfmid} {cellpath}
add_uniform_channel "KAf_channel" 60.5e-6 1000e-6 {gKAfdist} {cellpath}
// KAs in the soma and proximal dendrites
add_uniform_channel "KAs_channel" 0 16.1e-6 {gKAsprox_D1} {cellpath}
// KAs in the middle and distal dendrites
add_uniform_channel "KAs_channel" 16.1e-6 1000.0e-6 {gKAsdist_D1} {cellpath}
add_uniform_channel "KIR_channel" 0 16.1e-6 {gKIR_D1} {cellpath}
add_uniform_channel "KIR_channel" 16.1e-6 1000e-6 {gKIR_D1} {cellpath}
add_uniform_channel "K_DR" 0 16.1e-6 {gKDR_D1} {cellpath}
add_uniform_channel "K_DR" 16.1e-6 1000e-6 {gKDR_D1} {cellpath}
// function add_CaShells is defined in adjust.g
// to be coupled with N/Q/R Ca2+ channels
add_CaShells {CA_BUFF_1} 0 500e-6 {cellpath}
// to be coupled with T/L Ca2+ channels
add_CaShells {CA_BUFF_2} 0 500e-6 {cellpath}
// to be coupled with all Ca2+ channels
add_CaShells {CA_BUFF_3} 0 500e-6 {cellpath}
/************************************************************************
the parameters for Pbar of Calcium channels are adopted from Wolf's
2005 model. Please note in order to transfer the units into SI unites,
all parameters should be multiplied by 1e-2
************************************************************************/
// add_uniform_channel "CaQ_channel" 0 16e-6 {gCaQ} {cellpath}
add_uniform_channel "CaR_channel" 0 500e-6 {gCaR} {cellpath}
add_uniform_channel "CaN_channel" 0 16e-6 {gCaN_D1} {cellpath}
add_uniform_channel "CaL12_channel" 0 500e-6 {gCaL12_D1} {cellpath}
add_uniform_channel "CaL13_channel_D1" 0 500e-6 {gCaL13_D1} {cellpath}
add_uniform_channel "CaT_channel" 0 500e-6 {gCaT} {cellpath}
add_uniform_channel "BKK_channel" 0 500e-6 10 {cellpath}
add_uniform_channel "SK_channel" 0 500e-6 0.5 {cellpath}
end
//************************ End function add_channels ***********************
//**************************************************************************
//************************ End Local Subroutines ******************************
//*****************************************************************************
//************************ Begin Primary Routine ******************************
//*****************************************************************************
//************************ Begin function make_MS_cell *********************
//**************************************************************************
function make_MS_cell (cellpath,pfile)
str cellpath,pfile
echo {cellpath}
// function make_MS_cell is the first call from the primary file (MSsim.g).
// Note that the first thing it does is to call make_protypes in proto.g.
// These prototypes must be made before the call to add_channels. When the
// function add_channels is modified (as in msv4.0) to no longer add
// certain channels (such as K13, KRPI & KRPII), then the respective
// make_prototypes calls (i.e. make_KRPII_channel should be deleted as
// dead code. That is to say that only those channels shown in add_channels
// (above) should have a make prototype in function make_prototypes in
// proto.g
make_prototypes // proto.g
// readcell {pfile} {cellpath} -hsolve // see MScell.g
readcell {pfile} {cellpath}
set_position {cellpath} // local call
add_channels {cellpath} // local call
end
//************************ End function make_MS_cell ***********************
//**************************************************************************
//************************ End Primary Routine ********************************
//*****************************************************************************