/*******************************************************************
Load a cell's morphology file and then load Typc C biophysical model
2/18/2005
*******************************************************************/
/*--------------------playing random seed----------------*/
use_mcell_ran4(1)
lowindex = 1001
mcell_ran4_init(lowindex)
highindex = 1
print "------------------------------Type C Model-----------------------"
//flag for wether or not to use NMDA-R: 0 for without NMDA-R, 1 for with NMDA-R
//this flag will be used in subsequent hoc files: NregNsyn.hoc, Nregsyn.hoc etc
NMDAFLAG = 0
if (NMDAFLAG == 0) { //only AMPA-R
wtAmpa = 1
wtNmda = 0
} else { //NMDAFLAG = 1, with both AMPA-R, NMDA-R
wtAmpa = 0.8
wtNmda = 0.2
}
xopen("./c20466.hoc") // geometry file
soma area(0.5) //make sure diam reflects 3d points
//USER5MAX, APIDENDMAX, BASDENDMAX are set in cell's hoc file
TOTALDEND = USER5MAX + APIDENDMAX + BASDENDMAX + 3 //"3": counting start at 0
objref dend[TOTALDEND]
/****************************************************************************
Grouping dendritic sections together in an array dend, later for being used to
randomly put synapses on the proximal apical, distal apical and basal dendrite
*****************************************************************************/
index = 0
forsec "dendrite" { //this will includes both dendrites and apical-dendrites
dend[index] = new SectionRef()
index += 1
}
forsec "user5" {
dend[index] = new SectionRef()
index += 1
} //now index = USER5MAX + 1
//quadratic function fitting result A and B are set in cell's hoc file
A = A1
B = B1
//synapse rise and decay time constants
TAU1=0.2 //ms
TAU2=10 //ms
//cvode_active(1)
dist=1
rel=0.1
Rm = 28000 //unit: ohm-cm^2
RmDend = Rm/1
RmSoma = Rm
RmAx = Rm
Cm = 1
CmSoma= Cm
CmAx = Cm
CmDend = Cm*1
RaAll= 50
RaSoma=50
RaAx = 50
Vrest = -65
gna = .02
AXONM = 2
gkdr = 0.01
celsius = 35.0
KMULT = 0.025
KMULTP = 0.025
gcan=0.0//005
gcal=0.0//005
gcat=0.0//005
ghd=0.00005
nash=0
forsec "axon" {insert pas e_pas=Vrest g_pas = 1/RmAx Ra=RaAx cm=CmAx}
forsec "soma" {insert pas e_pas=Vrest g_pas = 1/RmSoma Ra=RaSoma cm=CmSoma}
forsec "dendrite"{insert pas e_pas=Vrest g_pas = 1/RmDend Ra=RaAll cm=CmDend}
forsec "user5" {insert pas e_pas=Vrest g_pas = 1/RmDend Ra=RaAll cm=CmDend}
access soma
freq=50
load_file("fixnseg.hoc")
geom_nseg()
tot=0
forall {tot=tot+nseg}
distance()
maxdist=0
forsec "user5" for(x) {if (distance(x)>maxdist) {maxdist=distance(x)}}
print "total # of segments (50Hz): ",tot, " max path distance: ", maxdist
objref sref, blist[USER5MAX+1], aplist
strdef dend2, trunk
for i=0, USER5MAX {
blist[i] = new Vector()
}
aplist = new Vector(APIDENDMAX+1)
//*********mapping bifurcations******************
for i=0, APIDENDMAX apical_dendrite[i] {
while (!issection("user5.*")) {
// print "before ", i, secname()
sref = new SectionRef()
access sref.parent
sprint(dend2, secname())
}
// print "apical ",i," ",dend2
for k=0, USER5MAX user5[k] {
sprint(trunk,secname())
x=strcmp(dend2, trunk)
if (x==0) {blist[k].append(i) aplist.x[i]=k}
}
}
//************************************************
forsec "axon" {
insert nax gbar_nax=gna * AXONM sh_nax=nash
insert kdr gkdrbar_kdr=gkdr
insert kap gkabar_kap = KMULTP*0.2
}
forsec "soma" {
insert hd ghdbar_hd=ghd vhalfl_hd=-73
insert na3 ar_na3=1 sh_na3=nash gbar_na3=gna
insert kdr gkdrbar_kdr=gkdr
insert kap gkabar_kap = KMULTP
}
forsec "dendrite" {
if(!issection("apical_dendrite.*")) {
insert hd ghdbar_hd=ghd vhalfl_hd=-73
insert na3 ar_na3=1 sh_na3=nash gbar_na3=gna
insert kdr gkdrbar_kdr=gkdr
insert kap gkabar_kap = KMULTP
}
}
forsec "user5" {
insert ds
insert hd ghdbar_hd=ghd
insert na3 ar_na3=1 gbar_na3=gna sh_na3=nash
insert kdr gkdrbar_kdr=gkdr
insert kap gkabar_kap=0
insert kad gkabar_kad=0
for (x) if (x>0 && x<1) {
xdist = distance(x)
if (xdist>350) {xdist=350}
ghdbar_hd(x) = ghd*(1+3*xdist/100)
if (xdist > 100){
vhalfl_hd=-81
gkabar_kad(x) = KMULT*(1+xdist/100)
} else {
vhalfl_hd=-73
gkabar_kap(x) = KMULTP*(1+xdist/100)
}
// print secname()," ",x, gkabar_kap(x), gkabar_kad(x)
}
}
for i=0, APIDENDMAX apical_dendrite[i] {
insert ds
insert hd
insert na3 ar_na3=1 gbar_na3=gna sh_na3=nash
insert kdr gkdrbar_kdr=gkdr
insert kap
insert kad
gkabar_kad = user5[aplist.x[i]].gkabar_kad(1)
gkabar_kap = user5[aplist.x[i]].gkabar_kap(1)
vhalfl_hd = user5[aplist.x[i]].vhalfl_hd
ghdbar_hd = user5[aplist.x[i]].ghdbar_hd(1)
// print secname()," ", gkabar_kap, gkabar_kad, ghdbar_hd
}
//correct the default section name
access soma
proc init() {
t=0
forall {
v=Vrest
if (ismembrane("nax") || ismembrane("na3")) {ena=55}
if (ismembrane("kdr") || ismembrane("kap") || ismembrane("kad")) {ek=-90}
if (ismembrane("hd") ) {ehd_hd=-30}
}
finitialize(Vrest)
fcurrent()
forall {
for (x) {
if (ismembrane("na3")||ismembrane("nax")){e_pas(x)=v(x)+(ina(x)+ik(x))/g_pas(x)}
if (ismembrane("hd")) {e_pas(x)=e_pas(x)+i_hd(x)/g_pas(x)}
}
}
cvode.re_init()
cvode.event(tstop)
access soma
// g.begin()
}
proc advance() {
fadvance()
// g.plot(t)
// g.flush()
// p.flush()
// doNotify()
}
init()