"Fluorescent protein voltage sensors are recombinant proteins that are designed as genetically encoded cellular probes of membrane potential using mechanisms of voltage-dependent modulation of fluorescence. Several such proteins, including VSFP2.3 and VSFP3.1, were recently reported with reliable function in mammalian cells. ... Expression of these proteins in cell membranes is accompanied by additional dynamic membrane capacitance, ... We used recordings of sensing currents and fluorescence responses of VSFP2.3 and of VSFP3.1 to derive kinetic models of the voltage-dependent signaling of these proteins. Using computational neuron simulations, we quantitatively investigated the perturbing effects of sensing capacitance on the input/output relationship in two central neuron models, a cerebellar Purkinje and a layer 5 pyramidal neuron. ... ". The Purkinje cell model is included in ModelDB.
Model Type: Neuron or other electrically excitable cell
Region(s) or Organism(s): Cerebellum
Cell Type(s): Cerebellum Purkinje GABA cell
Currents: I Na,t; I A; I K; I h; I K,Ca; I Calcium
Genes: Kv1.1 KCNA1; Kv4.3 KCND3; Kv3.3 KCNC3; Kv3.4 KCNC4; HCN1
Simulation Environment: NEURON
Implementer(s): Akemann, Walther [akemann at brain.riken.jp]
References:
Akemann W, Lundby A, Mutoh H, Knöpfel T. (2009). Effect of voltage sensitive fluorescent proteins on neuronal excitability. Biophysical journal. 96 [PubMed]