The ATP-sensitive K(+) (K(ATP)) channels are composed of sulfonylurea receptor and inwardly rectifying K(+) channel (Kir6.2) subunit. These channels are regulated by intracellular ADP/ATP ratio and play a role in cellular metabolism. ... The objective of this study was to determine whether Diethyl pyrocarbonate (DEPC) modifies K(ATP)-channel activity in pituitary GH(3) cells. ... Simulation studies also demonstrated that the increased conductance of K(ATP)-channels used to mimic DEPC actions reduced the frequency of spontaneous action potentials and fluctuation of intracellular Ca(2+). The results indicate that chemical modification with DEPC enhances K(ATP)-channel activity and influences functional activities of pituitary GH(3) cells. See paper for more and details.
Model Type: Neuron or other electrically excitable cell
Cell Type(s): Pituitary cell
Currents: I K; I K,Ca; I Potassium; ATP-senstive potassium current
Receptors: IP3
Genes: Kir6.2 KCNJ11
Model Concept(s): Activity Patterns; Ion Channel Kinetics; Action Potentials
Simulation Environment: XPPAUT
Implementer(s): Wu, Sheng-Nan [snwu at mail.ncku.edu.tw]; Chang, Han-Dong
References:
Wu SN, Chang HD. (2006). Diethyl pyrocarbonate, a histidine-modifying agent, directly stimulates activity of ATP-sensitive potassium channels in pituitary GH(3) cells. Biochemical pharmacology. 71 [PubMed]