"Human P2X7 receptors were expressed in Xenopus laevis oocytes and single channels were recorded using the patch-clamp technique in the outside-out configuration. ATP4- evoked two types of P2X7 receptor-mediated single channel currents characterized by short-lived and long-lived openings. ... The kinetics of the short channel openings at negative membrane potentials fitted well to a linear C-C-C-O model with two ATP4- binding steps at equal binding sites ...." and "Using the patch-clamp method, we studied the influence of external alkali and organic monovalent cations on the single-channel properties of the adenosine triphosphate (ATP)-activated recombinant human P2X(7) receptor." See the references for more.
Model Type: Channel/Receptor
Cell Type(s): Microglia; Macrophage; B lymphocyte
Model Concept(s): Ion Channel Kinetics; Nociception
Simulation Environment: QuB
Riedel T, Schmalzing G, Markwardt F. (2007). Influence of extracellular monovalent cations on pore and gating properties of P2X7 receptor-operated single-channel currents. Biophysical journal 93 [PubMed]
Riedel T, Lozinsky I, Schmalzing G, Markwardt F. (2007). Kinetics of P2X7 receptor-operated single channels currents. Biophysical journal 92 [PubMed]