Phosphoinositide-Dependent Signaling in Sympathetic Neurons (SCG) (Kruse et al. 2016)

Phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2] is a minor phospholipid in the cytoplasmic leaflet of the plasma membrane. Depletion of PI(4,5)P2 via phospholipase C-mediated hydrolysis leads to a decrease in exocytosis and alters electrical excitability in neurons. Restoration of PI(4,5)P2 is essential for a return to basal neuronal activity. However, the dynamics of phosphoinositide metabolism have not been analyzed in neurons. We studied the dynamics of phosphoinositide metabolism in sympathetic neu- rons upon muscarinic stimulation and used the kinetic information to develop a quantitative description of neuronal phospho- inositide metabolism. The measurements and analysis show a several-fold faster synthesis of PI(4,5)P2 in sympathetic neurons than in an electrically nonexcitable cell line, and provide a framework for future studies of PI(4,5)P2-dependent processes in neurons.

Currents: I M

Receptors: M1

Transmitters: Acetylcholine

Simulation Environment: Virtual Cell (web link to model)

Implementer(s): Kruse, Martin [mkruse at]


Kruse M, Vivas O, Traynor-Kaplan A, Hille B. (2016). Dynamics of Phosphoinositide-Dependent Signaling in Sympathetic Neurons. The Journal of neuroscience : the official journal of the Society for Neuroscience. 36 [PubMed]

This website requires cookies and limited processing of your personal data in order to function. By continuing to browse or otherwise use this site, you are agreeing to this use. See our Privacy policy and how to cite and terms of use.